In vitro comparsion between adipogenic differentiation of mesenchymal stem cells derived from human adipose tissue and amniotic fluid

Authors

  • Abd El Wahab El Ghareeb Zoology Department, Faculty of science, Cairo University, Cairo
  • Ahmed El Wahy Department of Chemistry, Faculty of Science, Cairo University, Egypt.
  • Mohamed Sobh Urology & Nephrology Center, Research Center, Mansoura University, Egypt.
  • Nada El Fatairy Department of Chemistry, Faculty of Science, Cairo University, Egypt.

Keywords:

Human adipose tissue, Amniotic fluid cells, Mesenchymal stem cells, Adipocyte stem cells, adipocyte gene, adiponectin gene

Abstract

Mesenchymal stem cells (MSCs) represent an archetype of multipotent somatic stem cells that hold promise for application in regenerative medicine. The present study aimed to isolate MSCs from adipose tissue and amniotic fluid (hAFSCs) and re-differentiation into either adipose. Adult human adipose tissue (hATSCs) contains a population of mesenchymal stem cells, which can be harvested readily, safely, and in relative abundance by modern liposuction techniques. MSCs were separated from adipose tissue liposuction and amniotic fluid, cultured in dullbecus modified egal media (DMEM) for two weeks for proliferation of MSCs, which recollected and regrowing in specific media for differentiation of adipocyte cells (ACs). ACs were determined by staining with oil-red O and RT PCR assessments of adipocyte and adiponectin genes.Our findings revealed that the MSCs derived from amniotic fluid cells showed high capacity of differentiation into adipocytes comparing with that derived from adipose tissue. The ACs derived from hAFSCs were more prominent and characterized by reddish brown-droplets following staining with oil red O. Both types of adipocyte stem cells derived from either hATSCs or hAFSCs showed similar expression of molecular bands of adponectin and adipocyte gene. The authors concluded that adipocytes derived from MSCs of hAFSCs were markedly growing and expanded comparing with that hATSCs for application in regenerative medicine.

 

References

• Caplan AI, Bruder SP. Mesenchymal stem cells: building blocks for molecular medicine in the 21st century. Trends Mol Med 2001;7:259-64.

• Leo AJ, Grande DA. Mesenchymal stem cells in tissue engineering. Cells Tissues Organs 2006;183:112-22.

• Caplan AI, Dennis JE. Mesenchymal stem cells as trophic mediators. J Cell Biochem 2006;98:1076-84

• .4- Meirelles L.S., Nardi N.B., 2009. Methodology, biology and clinical applications of mesenchymal stem cells. Front Biosci., 1;14:4281-98.

• Prusa AR, Marton E, Rosner M, Bernaschek G, Hengstschläger M: Oct-4- expressing cells in human amniotic fluid: a new source for stem cell research? Hum Reprod 2003, 18:1489-1493.

• Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH. Multilineage cells from human adipose tissue: Implications for cell-based therapies. Tissue Eng, 7, 2001, 211–226.

• Friedenstein AJ, Petrakova KV, Kurolesova AI, Frolova GP. Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. Transplantation 1968;6: 230-47.

• Stockl S, Bauer RJ, Bosserhoff AK, Gottl C, Grifka J, Grassel S. Sox9 modulates cell survival and adipogenic differentiation of multipotent adult rat mesenchymal stem cells. J Cell Sci 2013;126:2890-902.

• Urs S, Smith C, Campbell B, Saxton AM, Taylor J, Zhang B, Snoddy J, Jones Voy B and Moustaid-Moussa N. Gene expression profiling in human preadipocytes and adipocytes by microarray analysis. J Nutr, 134(4), 2004, 762-770.

• Yu S, Matsusue K, Kashireddy P, Cao WQ, Yeldandi V, Yeldandi AV, Rao MS, Gonzalez FJ and Reddy JK. Adipocyte-specific gene expression and adipogenic steatosis in the mouse liver due to peroxisome proliferator-activated receptor gamma1 (PPARgamma1) overexpression. J Biol Chem, 278(1), 2003, 498-505.

• Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop D, Horwitz E. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 2006;8:315-7.

• Maclimans WF, Dacis EV, Glover FL, Rake GW. The submerged culture of mammalian cells. The spinner culture. J. Immunol, 79, 1957, 428-430.

• 13.Bryder D, Rossi DJ, Weissman IL. Hematopoietic stem cells: The paradigmatic tissue-specific stem cell. Am J Pathol 2006; 169: 338-346.

• 14.Gimble JM, Katz AJ and Bunnell BA. Adipose-derived stem cells for regenerative medicine.Circulation Res., 100, 2007, 1249-1260.

• 15. Katz AJ and Bunnell BA. Adipose-derived stem cells for regenerative medicine. Circulation Res, 100, 2007, 1249-1260.

• 16. Phermthai T1, Odglun Y, Julavijitphong S, Titapant V, Chuenwattana P, Vantanasiri C, Pattanapanyasat K. A novel method to derive amniotic fluid stem cells for therapeutic purposes, BMC Cell Biology 2010, 11:79.

• 17.Schaffler A, Buchler C: Concise review: adipose tissue-derived stromal cells–basic and clinical implications for novel cell-based therapies. Stem Cells 2007, 25:818-827.

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Published

2016-10-18

Issue

Vol. 4 No. 5 (2016): October 2016

Section

Articles

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How to Cite

In vitro comparsion between adipogenic differentiation of mesenchymal stem cells derived from human adipose tissue and amniotic fluid. (2016). Asian Journal of Applied Sciences, 4(5). https://ajouronline.com/index.php/AJAS/article/view/4113

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