In vitro comparsion between adipogenic differentiation of mesenchymal stem cells derived from human adipose tissue and amniotic fluid

Authors

  • Abd El Wahab El Ghareeb Zoology Department, Faculty of science, Cairo University, Cairo
  • Ahmed El Wahy Department of Chemistry, Faculty of Science, Cairo University, Egypt.
  • Mohamed Sobh Urology & Nephrology Center, Research Center, Mansoura University, Egypt.
  • Nada El Fatairy Department of Chemistry, Faculty of Science, Cairo University, Egypt.

Keywords:

Human adipose tissue, Amniotic fluid cells, Mesenchymal stem cells, Adipocyte stem cells, adipocyte gene, adiponectin gene

Abstract

Mesenchymal stem cells (MSCs) represent an archetype of multipotent somatic stem cells that hold promise for application in regenerative medicine. The present study aimed to isolate MSCs from adipose tissue and amniotic fluid (hAFSCs) and re-differentiation into either adipose. Adult human adipose tissue (hATSCs) contains a population of mesenchymal stem cells, which can be harvested readily, safely, and in relative abundance by modern liposuction techniques. MSCs were separated from adipose tissue liposuction and amniotic fluid, cultured in dullbecus modified egal media (DMEM) for two weeks for proliferation of MSCs, which recollected and regrowing in specific media for differentiation of adipocyte cells (ACs). ACs were determined by staining with oil-red O and RT PCR assessments of adipocyte and adiponectin genes.Our findings revealed that the MSCs derived from amniotic fluid cells showed high capacity of differentiation into adipocytes comparing with that derived from adipose tissue. The ACs derived from hAFSCs were more prominent and characterized by reddish brown-droplets following staining with oil red O. Both types of adipocyte stem cells derived from either hATSCs or hAFSCs showed similar expression of molecular bands of adponectin and adipocyte gene. The authors concluded that adipocytes derived from MSCs of hAFSCs were markedly growing and expanded comparing with that hATSCs for application in regenerative medicine.

 

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Published

2016-10-18

How to Cite

El Ghareeb, A. E. W., El Wahy, A., Sobh, M., & El Fatairy, N. (2016). In vitro comparsion between adipogenic differentiation of mesenchymal stem cells derived from human adipose tissue and amniotic fluid. Asian Journal of Applied Sciences, 4(5). Retrieved from https://ajouronline.com/index.php/AJAS/article/view/4113

Issue

Vol. 4 No. 5 (2016): October 2016

Section

Articles

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