Assessment of the Zygote and Cleaved Stages Frozen-Thawed Human Embryos by Vitrification and their Survival to Blastocysts

Authors

  • Abd El Wahab El Ghareeb Zoology Department, Faculty of science, Cairo University, Cairo
  • Hamida Hamdi Zoology Department, Faculty of science, Cairo University, Cairo
  • Abdel-Latif Sweilam Department of Gynecology, Faculty of Medicine, Cairo University, Cairo
  • Niveen Ahmed Farah IVF Center, Cairo

Keywords:

Human zygote, cleaved stages, vitrification.

Abstract

Cryopreservation of human gametes and embryos is now considered as an important tool in assisted reproduction treatments as it increases the cumulative pregnancy rates while decreasing cost.

Cryopreservation procedures are such unphysiological circumstances mainly on account of severe temperature and osmotic alterations. Since the early 1990s, two common methods of cryopreservation have been used. Both of these methods have finally depended on the freezing and solidification of cell or tissue. Recently, the one known as vitrification has been claimed as the future of cryopreservation because of increased survival and success rates. However, this method is a non-equilibrium technique of cryopreservation that shows critical requirements of much higher concentration of permeable cryoprotectants and rate of cooling. Thus, it is a more vigorous mean of all possible cell damage except the formation of intracellular ice crystals that is totally prevented by vitrification. Nevertheless, there is no adequate cumulative data on the outcomes of vitrification performed at different stages of human embryos.

The aim of this study a) is to assess whether vitrification at different early stages (zygotes and cleavage) of human embryos alters the outcomes of vitrification. b) if so, to predict the preferable stage between the two stages of vitrification of human embryos to attain the highest yields. It has been well demonstrated that vitrification of human gametes and embryos have resulted in different success rates according to the developmental stage of the cell. Then, the most preferable stage of vitrification will be identified on the basis of blastocyst formation obtaining from extended culture conditions of the thawed embryos till day 5, using non-transferable embryos.

References

• Al-Hasani S, Ludwig M, Gagsteiger F et al. 1996 Comparison of cryopreservation of supernumerary pronuclear human oocytes obtained after intracytoplasmic sperm injection (ICSI) and after conventional in-vitro fertilization. Human Reproduction 11, 604–607.

• Arav A, Zeron Y.Vitrification of bovine oocytes using modified minimum drop size technique (MDS) is effected by the composition and the concentration of the vitrification solution and by the cooling condition. Theriogenology 1997;46:1.

• Boldt J, Cline D, McLaughlin D 2003 Human oocyte cryopreservation as an adjunct to IVF-embryo transfer cycles. Human Reproduction18, 1250–1255.

• El-Danasouri I, Selman H 2001 Successful pregnancies and deliveries after a simple vitrification protocol for day 3 human embryos. Fertility and Sterility 76, 400–402.

• Fabbri R, Porcu E, Marsella T et al. 2001 Human oocyte cryopreservation: new perspectives regarding oocyte survival. Human Reproduction 16, 411–416.

• Fosas N, Marina F, Torres PJ et al. 2003 The births of five Spanish babies from cryopreserved donated oocytes. Human Reproduction18, 1417–1421.

• Graham J, Han T, Porter R, Levy M,Stillman R, et al. Day 3 morphology is a poor predictor of blastocyst quality in extended culture. FertilSteril 2000;74:495–7.

• Hiraoka K, Kinutani M, Kinutani K. Blastocoele collapse by micropipetting prior to vitrification gives excellent survival and pregnancy outcomes for human day 5 and 6 expanded blastocysts. Hum Reprod 2004;19(12):2884–8.

• Horne G, Critchlow JD, Newman MC et al. 1997 A prospective evaluation of cryopreservation strategies in a two-embryo transfer programme. Human Reproduction 12, 542–547.

• Huang CC, Lee TH, Chen HH et al. 2005 Successful pregnancy following blastocyst cryopreservation using super-cooling ultrarapidvitrification. Human Reproduction 20, 122–128.

• Isachenko V, Selman H, Isachenko E, et al. Modified vitrification of human pronuclear oocytes: efficacy and effect on ultrastructure. Reprod Biomed Online 2003;7:211–6.

• Kattera S, Shrivastav P, Craft I 1999 Comparison of pregnancy outcome of pronuclear- and multicellular-stage frozen–thawed embryo transfers. Journal of Assisted Reproduction and Genetics16, 358–362.

• Koutlaki N, Schoepper B, Maroulis G et al. 2006 Human oocyte cryopreservation: past, present and future. Reproductive BioMedicine Online 13, 427–436

• Kuleshova L, Lopata A 2002 Vitrification can be more favorable than slow cooling. Fertility and Sterility 78, 449–454.

• KuwayamaM, Vajta G, Ieda S, Kato O. Comparison of open and closed methods for vitrification of human embryos and the elimination of potential contamination. Reprod Biomed Online 2005;11:608–14.

• Kuwayama, M.; Vajta, G.; Kato, O,; and Leibo, S. P. (2005): "Highly efficient vitrification method for cryopreservation of human oocytes," Reproductive BioMedicine Online, 11 (3): 300-308.

• Kuwayama, M. (2007): Highly efficient vitrification for cryopreservation of human oocytes and embryos: the cryotop method. Theriogenology, 67:73-80.

• Kuwayama, M. (2009): The human oocyte: Vitrification. In Gardner, D.K.; weissman, A.; Howles, C.M. and Shoham, Z. (3rd Eds.), textbook of assisted reproductive technologies: Laboratory and clinical perspectives. Informa UK Ltd,; 3(19): 267-247.

• Liebermann J, Tucker MJ 2002 Effect of carrier system on the yield of human oocytes and embryos as assessed by survival and developmental potential after vitrification. Reproduction 124, 483–489.

• Liebermann and Tucker (2004). Vitrifying and warming of human oocytes, embryos and blastocysts: vitrification procedures as an alternative to conventional cryopreservation methods MolBiol 254:345-364.

• Mazur P 1990 Equilibrium, quasi-equilibrium, and nonequilibrium freezing of mammalian embryos. Cell Biophysics 17, 53–92.

• McWillams RB, Gibbons WE, Leibo SP. Osmotic and physiological responses of mouse zygotes and human oocytes to mono- and disaccharides. Hum Reprod 1995;10:1163–71.

• Milki AA, Hinckley MD, Fisch JD, et al. Comparison of blastocyst transfer with day 3 embryo transfer in similar patient populations.FertilSteril 2000;73:126–9.

• Mukaida T, Wada S, Takahashi K et al. 1998 Vitrification of human embryos based on assessment of suitable conditions for 8-cell mouse embryos. Human Reproduction 13, 2874–2879.

• Ozmen B, Schultze-Mosgau A, Youssry M, Diedrich K, Al-Hasani S, Unlu C 2007. Current data on vitrification of human embryos: which one is the best; Zygote, cleavage or blastocyst stage? J Turkish-German Gynecol Assoc. 8, 218-226.

• Queenan JTJ, Ramey JW, Seltman HJ et al. 1997 Transfer of cryopreserved–thawed pre-embryos in a cycle using exogenous steroids without prior gonadotrophin-releasing hormone agonist suppression yields favourable pregnancy results. Human Reproduction 12, 1176–1180.

• Rall WF, Fahy GM 1985 Ice-free cryopreservation of mouse embryos at –196°C by vitrification. Nature 313, 573–575.

• Saito H, Ishida GM, Kaneko T, Kawachiya S, Ohta N, Takahashi T, et al. Application of vitrification to human embryo freezing. GynecolObstet Invest 2000;49:145–9.

• Senn A, Vozzi C, Chanson A et al. 2000 Prospective randomized study of two cryopreservation policies avoiding embryo selection: the pronucleate stage leads to a higher cumulative delivery rate than the early cleavage stage. Fertility and Sterility 74, 946–952.

• Stehlik E, Stehlik J, Katayama KP et al. 2005 Vitrification demonstrates signifi cant improvement versus slow freezing of human blastocysts. Reproductive BioMedicine Online 11, 53–57.

• Tucker et al. (2003).Cryopreservation protocols. In Color Atlas of Human Assisted Reproduction: Laboratory and Clinical Insights, eds. P Patrizio, V Guelman and MJ Tucker, pp 257-276, Lippincott, Williams and Wilkins, Philadelphia.

• Vajta G, Lewis IM, Kuwayama M et al. 1998 Sterile application of open pulled straw (OPS) vitrification method. Cryo-Letters 19, 389–392.

• Vajta G, Nagy ZP 2006 Are programmable freezers still needed in the embryo laboratory? Review on vitrification. Reproductive BioMedicine Online 12, 779–796.

• Veeck L, Admundson CH, Brothman LJ et al. 1993 Significantly enhanced pregnancy rates per cycle through cryopreservation and thaw of pronuclear stage oocytes. Fertility and Sterility 59, 1202–1207.

• Yokota Y, Sato S, Yokota M, Ishikawa Y, Makita M, Asada T, et al. Successful pregnancy following blastocyst vitrification: case report. Hum Reprod 2000;15:1802–3.

• Zheng WT, Zhuang GL, Zhou CQ et al. 2005 Comparison of the survival of human biopsied embryos after cryopreservation with four different methods using non-transferable embryos. Human Reproduction 20, 1615–1618.

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Published

2016-08-31

How to Cite

El Ghareeb, A. E. W., Hamdi, H., Sweilam, A.-L., & Ahmed, N. (2016). Assessment of the Zygote and Cleaved Stages Frozen-Thawed Human Embryos by Vitrification and their Survival to Blastocysts. Asian Journal of Applied Sciences, 4(4). Retrieved from https://ajouronline.com/index.php/AJAS/article/view/4101

Issue

Vol. 4 No. 4 (2016): August 2016

Section

Articles

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