Pituitary FSHβ and GH and Ovarian GH-r, IGF-1 and IGF-2 Gene Expression Levels in Cycling Female Rats Immunized against Steroid-Free Bovine Follicular Fluid Antisera
Keywords:
Pituitary, Follicular fluid, FSHβ gene, GH, IGFs, inhibin, activinAbstract
To examine the comparative effects of inhibin-free and/or steroid-free bovine follicular fluid antisera; IS-FBFF or S-FBFF, at proestrus or metestrus, on pituitary FSHβ and GH as well as ovarian GH-r, IGF-1 and IGF-2 gene expression levels, fifty cycling virgin female rats were randomly assigned equally to control and four treated group. Control females were intraperitonealy injected with 100 µl of normal saline at proestrus, whereas treated were intraperitonealy injected with 100 µl of S-FBFF at proestrus (T1), IS-FBFF at metestrus (T2), S-FBFF at metestrus (T3), and IS-FBFF at metestrus (T4). At the end of proestrus, females were euthanized; pituitaries and ovaries were dissected for assessment pituitary FSHβ and GH, as well as ovarian GH-r, IGF-1 and IGF-2 gene expression levels. The results illustrated significant increase of the expression levels of all studied genes in T3 group than others, whereas T4 group recorded the lowest levels among experimental groups. In T1 group, significant decrease has been shown for the expression levels of pituitary FSHβ and ovarian IGF-2 genes, and significant decrease of pituitary GH and ovarian IGF-1 genes, whereas T2 group recorded significant decrease of pituitary FSHβ and GH and ovarian IGF-2 genes and significant increase of IGF-1 gene expression in comparison with control. It can be concluded that immunization against S-FBFF could increase the expression levels that related to ovarian growth and proliferation.
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