GUS Gene Expression in Somatic Embryo of Oil Palm (Elaeis guineensis Jacq.)

Authors

  • T. S. Mariani School of Life Sciences and Technology, Bandung Institute of Technology, Ganesha 10, Bandung 40132, Indonesia
  • D. Ermavitalini
  • T. Mitsutaka
  • T. F. Chia
  • H. Miyake

Abstract

Oil palm is an oil producing plant, which produce 5 to 7 tons of oil/ acre/ year. In this study, we carried out transformation of oil palm by GUS gene. The expression of GUS gene was obtained in 25 minutes soaking and 5 minutes vacuum infiltration method. The colour of GUS gene expression is blue.

Author Biography

  • T. S. Mariani, School of Life Sciences and Technology, Bandung Institute of Technology, Ganesha 10, Bandung 40132, Indonesia
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References

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o Mariani, T.S., Sjafrullatif, Wardjo, N.G., Miyake, H. 2014a. Initiation and proliferation of globular somatic embryos of oil palm (Elais guineensis Jacq.) observed by inverted and transmission electron microscopy. International Journal of Basic and Applied Sciences. Vol 14 (01):1-7

o Mariani, T.S., Sasmitamihardja, D.,Mienanti,D., Sjafrullatif, Ginting, G., Miyake, H. 2014b. Somatic embryogenesis of oil palm (Elaeis guineensis Jacq.) for synthetic seed production. Asian Journal of Applied Sciences. Vol 02(03): 358-367

o Muad, M.R. (2000). Genetic transformation of oil palm by chitinase gene mediated by Agrobacterium tumefaciens. School of Bioscience and Biotechnology, Faculty of Science and Technology, UKM

o Parveez, G.K.A., Chowdhury, M.K.U, and Saleh, N.M. (1998). Biological parameters affecting transient GUS gene expression in oil palm (Elaeis guineensis Jacq.) embryogenic calli via microprojectile bombardment, An International Journal Industrial Crops and Products. 8:17-27

o Steeves, T.A. and Sussex, I.M. 1994. Patterns in Plant Development. Second Edition. Cambridge University Press.

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Published

2015-10-26

How to Cite

GUS Gene Expression in Somatic Embryo of Oil Palm (Elaeis guineensis Jacq.). (2015). Asian Journal of Applied Sciences, 3(5). https://ajouronline.com/index.php/AJAS/article/view/3109