Nucleotide and Amino Acid Sequencing of a Coat Protein of an Egyptian Isolate of Potato Virus Y (PVY)

Authors

  • Hatem El Mahdy Botany Department, Faculty of science, Cairo University, Cairo
  • Sayed Abd El Salam Botany Department, Faculty of science, Cairo University, Cairo

Keywords:

PVY, Detection, Polymerase chain reaction, cp gene, sequence

Abstract

We are reporting here a molecular tool for detection and identification of a PVY isolate by Nucleotide and amino acid sequencing of coat protein gene. The total RNA was isolated from PVY infected Solanum tuberosum L. leaves and then used as a template for RT-PCR to amplify the cDNA followed by amplification of cp gene (801bp). This followed by sequencing of PVY cp gene and comparing it with (29) PVY overseas strains or isolates. The similarity between our PVY Egyptian isolate and the (29) overseas strains or isolates ranged from 24.4 to 99.4% and from  98.1 to 99.3% based on the level of DNA and deduced amino acids sequence, respectively. In conclusion, our Egyptian virus isolate is PVY strain N.

References

• Abdel-Salam, A. M.; El-Attar, A. K. and Gambley, C. F. (2014). Production of polyclonal antisera to a recombinant coat protein of Potato virus Y expressed in Escherichia coli and its application for immunodiagnosis. International Journal of Virology 10(1):1-16.

• Al-Saikhan, M. S.; Alhudaib, K. A. and Soliman, A. M. (2014). Detection of three potato viruses isolated from Saudi Arabia. International Journal of Virology 10 (3):224-234.

• Budzanivska, I. G.; Ovcharenko, L. P.; Kharina, A. V.; Boubriak, I. I. and Polischuk, V. P. (2014). Nucleotide and amino acid sequences of a coat protein of an Ukrainian isolate of Potato virus Y: comparison with homologous sequences of other isolates and phylogenetic analysis. Biopolymers and Cell 30(2): 141-148.

• Food and Agriculture Organitation of the united nations. (2012).FAOSTAT.http://faostat.fao.org/site/339/default.aspx. Accessed 6 February 2013.

• Gao FangLuan; Chang Fei; Shen JianGuo; Shi FengYang; Xie LianHui and Zhan JiaSui (2014). Complete genome analysis of a novel recombinant isolate of potato virus Y from China. Archives of Virology 159(12):3439-3442.

• Gray, S.M., S. De Boer, J. Lorenzen, A. Karasev, J. Whitworth, P. Nolte, R. Singh, A. Boucher and H. Xu. (2010). Potato virus Y: An evolving concern for potato crops in the United States and Canada. Plant Disease 94(2): 1384-1397.

• Hu, X., Meacham, T., Ewing, L., Gray, S. M. and Karasev, A. V. (2009). A novel recombinant strain of Potato virus Y suggests a new viral genetic determinant of vein necrosis in tobacco. Virus Research 143, 68-76.

• Kamangar, S. B.; Smagghe, G.; Maes, M. and Jonghe, K. de (2014). Potato virus Y (PVY) strains in Belgian seed potatoes and first molecular detection of the N-Wi strain. Journal of Plant Diseases and Protection 121 (1): 10-19.

• Le Romancer, M., C. Kerlan and M. Nedellec. (1994). Biological characterisation of various geographical isolates of potato virus Y inducing superficial necrosis on potato tubers. Plant Pathology 43: 138-144.

• Margaritopoulos, J. T., Dovas, C. I., Gounaris, J,. Skouras, P. J., Kanavaki, O. M., Katis, N. I. and Tsitsipis, J. A. (2009). Molecular analysis of the coat protein of Potato virus Y isolates in Greece suggests multiple introductions from different genetic pools. Journal of Phytopathology Published Online: Apr 30 2009 2:27AM. DOI: 10.1111/j.1439-0434.2009.01579.x.

• Massumi, H., Shaabanian, M., Pour, A. H,. Heydarnejad, J. and Rahimian, H. (2009). Incidence of viruses infecting tomato and their natural hosts in the Southeast and central regions of Iran. Plant Disease 93, 67-72.

• Moodley, V.; Ibaba, J. D.; Naidoo, R. and Gubba, A. (2014). Full-genome analyses of a Potato Virus Y (PVY) isolate infecting pepper (Capsicum annuum L.) in the Republic of South Africa. Virus Genes 49(3):466-476. 66 ref.

• Mousavi, L.; Mozafari, J.; Rakhshandehroo, F.; Amir Modarresi Chahardehi and Mousavi, M. (2014). Distribution and prevalence Potato virus Y isolates obtained from potatoes grown in the Iran by RT-PCR. Journal of Biology, Agriculture and Healthcare 4(4):45-53.

• Pooja Sharma; Sahu, A. K.; Verma, R. K.; Ritesh Mishra and Gaur, R. K. (2013). Biological and molecular characterization of Potato virus Y infecting potato (Solanum tuberosum) in India. Asian Journal of Biological Sciences 6(5):257-264.

• Sadik, A. S.; Hussein, A. H.; Abdel-Hamid, I.A.; Gabr, G.A.; Allah, S.O. (2003). A serological and molecular studies on a potato virus Y potyvirus isolate. Annals of Agricultural science, Cairo 48 (2): 505-532.

• Sambrook, J.L.; Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual and Edn. New York: Cold Spring Harbor Laboratory Press, pp.160.

• Sun-Qi and Zhang-ChunQing. (2005). Studies on the methods of detecting PVYN and PVYO by RT-PCR. Scientia Agricultura Sinica 38(1): 213-216.

• Takacs, A. (1999). Morphology, genetics and strains of the potato Y potyvirus. Novenytermeles 48(2): 199-208.

• Tomczynska, I.; Jupe, F.; Hein, I.; Marczewski, W. and Sliwka, J. (2014). Hypersensitive response to Potato virus Y in potato cultivar Sarpo Mira is conferred by the Ny-Smira gene located on the long arm of chromosome IX. Molecular Breeding 34(2):471-480.

• United States Department of Agriculture. (2012). Potatoes. United States Department of Agriculture, Economic Research Services. Accessed 10 February 2013.

• Urcuqui-Inchima, S., Haenni, A.-L. and Bernardi, F. (2001). Potyvirus proteins: a wealth of functions. Virus Research 74, 157-175.

• Van Regenmortel-M.H.V. (1986). Tobacco mosaic virus: antigenic structure. In: M.H.V. Van Regenmortel and H. Frankel-Contrat Editors, The plant viruses, Plenum Press, New York and London pp. 79-104.

• Van Regenomrotel-M.H.V.; Fauguet C.M. and Bishopi, D.H.L. (Eds.) (2000). Virus Taxonomy. Clarification and Nomenclature of viruses. Seventh Report an taxonomy of viruses. Academic Press.

• Were, H. K.; Kabira, J. N.; Kinyua, Z. M.; Olubayo, F. M.; Karinga, J. K.; Aura, J.; Lees, A. K.; Cowan, G. H. and Torrance, L. (2013). Occurrence and distribution of potato pests and diseases in Kenya. Potato Research 56(4):325-342.

• Yardmci, N.; Klc, H. C. and Ozdemir, T. (2014). Detection of (PVY) potato Y potyvirus, on potato cultivars using biological and molecular methods growing in South-West Turkey. JAPS, Journal of Animal and Plant Sciences 24(5):1525-1530. 30 ref.

Downloads

Published

2016-04-16

How to Cite

El Mahdy, H., & Abd El Salam, S. (2016). Nucleotide and Amino Acid Sequencing of a Coat Protein of an Egyptian Isolate of Potato Virus Y (PVY). Asian Journal of Applied Sciences, 4(2). Retrieved from https://ajouronline.com/index.php/AJAS/article/view/3660

Issue

Vol. 4 No. 2 (2016): April 2016

Section

Articles

License

  • Papers must be submitted on the understanding that they have not been published elsewhere (except in the form of an abstract or as part of a published lecture, review, or thesis) and are not currently under consideration by another journal published by any other publisher.
  • It is also the authors responsibility to ensure that the articles emanating from a particular source are submitted with the necessary approval.
  • The authors warrant that the paper is original and that he/she is the author of the paper, except for material that is clearly identified as to its original source, with permission notices from the copyright owners where required.
  • The authors ensure that all the references carefully and they are accurate in the text as well as in the list of references (and vice versa).
  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Attribution-NonCommercial 4.0 International that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
  • The journal/publisher is not responsible for subsequent uses of the work. It is the author's responsibility to bring an infringement action if so desired by the author.