Production of Antibodies Specific to an Egyptian Isolate of Potato Virus Y (PVY)

Authors

  • Hatem El Mahdy Botany Department, Faculty of science, Cairo University, Cairo
  • Sayed Abd El Salam

Keywords:

PVY, Detection, ELISA

Abstract

We are reporting here serological tool for detection and identification of a PVY isolate. For the virus isolate detection a number of 3 potato plants (Solanun tuberosum L.) exhibiting rolling, necrotic local lesions and faint mosaic symptoms were collected from three different locations from open fields near Giza city, Egypt in order to detect the presence of PVY, PVX and PLRV for indirect DAS-ELISA test. IgG specific to the viral isolate labeled with alkaline phosphatase was raised. The prepared IgG showed specificity for the virus isolate and there is no serological relationship corresponding to PLRV and PVX.

References

• Adams, M.J., F.M. Zerbini, R. French, F. Raberstein, D.C. Stenger and J.P.T. Valkonen. (2011). Potyvirus. In: Virus taxonomy: Ninth report of the international committee on taxonomy of viruses, ed. A.M.Q. King, M.J. Adams, E.B. Carstens, and E.J. Lefkowitz, 1072-1078. Oxford: Elsevier.

• Abdel-Halim, M.M.; A.S. Sadik, M.I. Salama and A.M. Salem (2000). Isolation and characterization of an Egyptian strain of potato virus Y. Arab J. Biotech. 3: 115-132.

• Albrechtsen, S. E. (2006). Testing methods for seed-transmitted viruses: Principles and protocols. CABI Publishing. Oxfordshire, UK. pp 47-81; 84-147; 153-219.

• Al-Saikhan, M. S.; Alhudaib, K. A. and Soliman, A. M. (2014). Detection of three potato viruses isolated from Saudi Arabia. International Journal of Virology 10 (3):224-234.

• Chiunga, E. and Valkonen, J. P. T. (2013). First report of five viruses infecting potatoes in Tanzania. Plant Disease 97(9):1260-1261.

• Clark, M.F. and Adams, N.E. (1977). Characteristics of the microtiter plate method of enzyme-linked immunosorbent assay (ELISA) for the detection of plant viruses. J. Gen. Virol. 37: 475-483.

• Food and Agriculture Organitation of the united nations. (2012). FAOSTAT. http://faostat.fao.org/site/339/default.aspx. Accessed 6 February 2013.

• Garcia-Lampasona, S.C. and Gracia, O. (2000). Spread of potato Y potyvirus (PVY) in potato seed crops. Horticultura Argentina 18(44/45): 16-19.

• Gugerli, P. And Fries, P. (1983). Characterization of monoclonal antibodies to potato virus Y and their use for virus detection. J. Gen. Virol. 64: 2471-2477.

• Harrison, B.D. (1985). Usefulness and limitations of the species concept for plant viruses. Intervirology 24: 71-78.

• Hollings, M.L. and Brunt, A.A. (1981). Potyviruses. In: Kurstak E., ed. Handbook of plant virus infection comparative diagnosis. Elsevier North Halland, Amsterdam, PP. 731-807.

• Hutton, F.; Kildea, S.; Griffin, D.; Spink, J.; Doherty, G. and Hunter, A. (2013). First report of potato tuber necrotic ringspot disease associated with PVY recombinant strains in Ireland. New Disease Reports 12:28.

• Jordan, R. and Hammond, J. (1991). Comparison and differentiation of potyvirus isolates and identification of strain-, virus-, subgroup-, specific and potyvirus group-common epitopes using monoclonal antibodies. J. Gen. Virol. 72: 25-36.

• Kamangar, S. B.; Smagghe, G.; Maes, M. and Jonghe, K. de (2014). Potato virus Y (PVY) strains in Belgian seed potatoes and first molecular detection of the N-Wi strain. Journal of Plant Diseases and Protection 121 (1): 10-19.

• Koenig, R.C. and H.L. Paul (1982). Detection and differentiation of plant viruses by various ELISA procedures. Acta. Horticulture 127: 147-158.

• Margaritopoulos, J. T., Dovas, C. I., Gounaris, J., Skouras, P. J., Kanavaki, O. M., Katis, N. I. and Tsitsipis, J. A. (2009). Molecular analysis of the coat protein of Potato virus Y isolates in Greece suggests multiple introductions from different genetic pools. Journal of Phytopathology Published Online: Apr 30 2009 2:27AM. DOI: 10.1111/j.1439-0434.2009.01579.x.

• Murphy, F.A.; Fauquet, C.M.; Bishop, D.H.L.; Ghabrial, S.A.; Jarvis, A.W.; Martelli, G.P.; Mayo, M.A. and Summers, M.D. (1995). Classification and Nomenclature of Viruses, Sixth Report of the International Committee on Taxonomy of viruses. Springer Verlag Wien New York, pp. 348-354.

• Nie XianZhou and Mathuresh Singh (2013). Response of potato, tobacco and Physalis floridana plants to mixed infection with PVX, PVYNTN/ and PVY degrees strains. Canadian Journal of Plant Pathology 35(3):390-401.

• Pooja Sharma; Sahu, A. K.; Verma, R. K.; Ritesh Mishra and Gaur, R. K. (2013). Biological and molecular characterization of Potato virus Y infecting potato (Solanum tuberosum) in India. Asian Journal of Biological Sciences 6(5):257-264.

• Shukla, D. D., Ward, C. W. and Brunt, A. A. (1994). The potyviridae. CAB International. Oxon, UK. pp. 1-2; 322-328.

• Syller, J. and Grupa, A. (2014). The effects of co-infection by different Potato virus Y (PVY) isolates on virus concentration in solanaceous hosts and efficiency of transmission. Plant Pathology 63(2):466-475.

• Takacs, A. (1999). Morphology, genetics and strains of the potato Y potyvirus. Novenytermeles 48(2): 199-208.

• Tomczynska, I.; Jupe, F.; Hein, I.; Marczewski, W. and Sliwka, J. (2014). Hypersensitive response to Potato virus Y in potato cultivar Sarpo Mira is conferred by the Ny-Smira gene located on the long arm of chromosome IX. Molecular Breeding 34(2):471-480.

• United States Department of Agriculture. (2012). Potatoes. United States Department of Agriculture, Economic Research Services. Accessed 10 February 2013.

• Liave, C.; Martinez, B.; Diaz Ruiz, J.R. and Lopez-Abella, D. (1999). Serological analysis and coat protein sequence determination of potato virus Y (PVY) pepper pathotypes and differentiation from other PVY strains. Eur. J. Plant Pathol. 105: 847-857.

• Liu, F.B.; Sukhacheva, E.; Erokhina, T. and Schubert, J. (1999). Detection of potyviral nuclear inclusion b proteins by monoclonal antibodies raised to synthetic peptides. Eur. J. Plant Pathol. 105: 389-395.

• Varveri, C. (2000). Potato Y Potyvirus detection by immunological and molecular techniques in plants and aphides. Phytoparasitica 28: 141-148

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Published

2016-04-16

How to Cite

El Mahdy, H., & Abd El Salam, S. (2016). Production of Antibodies Specific to an Egyptian Isolate of Potato Virus Y (PVY). Asian Journal of Applied Sciences, 4(2). Retrieved from https://ajouronline.com/index.php/AJAS/article/view/3658

Issue

Vol. 4 No. 2 (2016): April 2016

Section

Articles

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