Using 16S rRNA Identification of an Endo-β-1,4-Glucanase Producing Endophyte from <i>Brachytrupes membranaceus</i> Gut
Keywords:
Brachytrupes membranaceus, gut, cellulase, Enterobacter asburiae, 16S rDNAAbstract
This work was focusing on bioprospecting for cellulase producing endophytes from the gut of Brachytrupes membranaceus. Identification of bacterial strains that have the capability of efficiently and effectively hydrolyzing the cellulosic macromolecular structure has realized a microbe that is a mesophilic. The microbe was characterized using 16S rDNA gene amplification and sequencing for phylogenetic relatedness. The 16SrRNA gene amplimer was sequenced using nucleotide sequencing on ABI 3100 DNA Analyser capillary electrophoresis sequencing system. The sequence was used for BLASTN search bioinformatics tools and noted that the sequence had 97 % homology with the Enterobacter asburiae. Such phylogenetic relatedness has subspecies differences that can be identified at the genomic level. The organism can grow optimally at pH 6.0 and temperature 40 ºC. Crude cellulase activity showed an optimum enzymatic activity conditions which were exactly the same to growth conditions. The enzymes will be characterized and evaluated for their potential application.References
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