</i>Toll-Like Receptor-2 </i>Gene <i>[TLR2]</i> Polymorphism and Susceptibility to <i>Mycobacteria Tuberculosis </i>Infections in Sudanese Patients

Authors

  • S. M. H. Mohammed
  • E. A. G. Khalil Department of Clinical Pathology & Immunology, Institute of Endemic Diseases, University of Khartoum
  • F. M. Idris

Keywords:

Background & Aim, Tuberculosis [TB] is a major public health threat and a leading cause of death worldwide. Innate immunity plays an important role in the host defense against M. tuberculosis through recognition Toll-Like Receptors [TLRs]. Polymorphism

Abstract

Tuberculosis [TB] is a major public health threat and a leading cause of death worldwide. Innate immunity plays an important role in the host defense against M. tuberculosis through recognition Toll-Like Receptors [TLRs]. Polymorphisms in TLR2 [Arg753Gln] gene have been reported to be associated with increased susceptibility to TB. This study aimed to determine possible roles for TLR2 [Arg753Gln] gene polymorphism in susceptibility to M. tuberculosis infections in a cohort of Sudanese individuals. Following informed consent, 141 individuals with smear positive tuberculosis and latent TB infections [mean age 19±38.3 years; male: female ratio  2:1]  and 197   apparently healthy individuals [mean age 30±14; male female ration of 1:5] were enrolled. DNA was extracted from EDTA-blood using the guanidine chloride method. PCR was carried out using TLR2 gene specific primers, Forward: [5'CATTCCCCAGCGCTTCTGCAAGCTCC-3'] and Reverse: [5'GGAACCTAGGACTTTATCGCAGCTC-3')]. Genotyping was done by RFLP using MSP1restriction enzyme. The TLR2 Arg753Gln mutant allele was not detected in any of the study groups. TLR2 Arg753Gln gene  polymorphisms plays little or no role in host susceptibility to Mycobacterium tuberculosis infections  in Sudanese population.

 

References

Cole ST, Brosch R, Parkhill J, Garnier T, Churcher C, Harris D. et al. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature; 393, 537-544 1998. | doi:10.1038/31159.

WHO Global Tuberculosis Control 2011. http://www.WHO.int/tb/publications/global_report/en [cited 2012 Jul 1]

Newport MJ, Huxley CM, Huston S, Hawrylowicz CM, Oostra BA, Williamson R, Levin M. A mutation in the interferon- gamma-receptor gene and susceptibility to mycobacterial infection. N Engl J Med 1996 ; 335: 1941-1949. 1996.

Dupuis S, Dargemont C, Fieschi C, Thomassin N, Rosenzweig S, Harris J, Holland SM, Schreiber RD, Casanova JL. 2001. Impairment of mycobacterial but not viral immunity by a germline human STAT1 mutation. Science; 293: 300-303.2001.

Means TK, Jones BW, Schromm AB, et al. Differential effects of a Toll-like receptor antagonist on Mycobacterium tuberculosis-induced macrophage responses. J Immunol;166:4074–4082.2001.

Supajatura V, Ushio H, Nakao A, et al. Differential responses of mast cell Toll-like receptors 2 and 4 in allergy and innate immunity. J Clin Invest; 109:1351–1359. 2002.

Thoma-Uszynski S, Stenger S, Takeuchi O, et al. Induction of direct antimicrobial activity through mammalian Toll-like receptors. Science; 291:1544–1547. 2001.

Lorenz E, Mira JP, Cornish KL, Arbour NC, Schwartz DA. A novel polymorphism in the Toll-like receptor 2 gene and its potential association with staphylococcal infection. Infect Immun; 68:6398–6401. 2000.

Ogus, AC, Yoldas B, Ozdemir T, Uguz A, Olcen S, Keserz I, Coskun M, Cilli A, Yegin O .The Arg753Gln polymorphism of the human Toll-like receptor 2 gene in tuberculosis disease. Eur Respir J; 23:219–223. 2004.

Xue Y, Zhao ZQ, Wang HJ, Jin L, Liu CP, Wang Y, Li JC. Toll-like receptors 2 and 4 gene polymorphisms in a southeastern Chinese population with tuberculosis. Int J Immunogenet;37:135-8. 2010.

Ryu YJ, Kim EJ, Koh WJ, Kim H, Kwon OJ, Chang JH . Toll-like receptor 2 polymorphisms and nontuberculous mycobacterial lung diseases. Clin Vaccine Immunol; 13:818-819. 2006.

Biswas D, Gupta SK, Sindhwani G, Patras A. TLR2 polymorphisms, Arg753Gln and Arg677Trp, are not associated with increased burden of tuberculosis in Indian patients. BMC Res Notes; 18:162. 2009.

Velez DR, Wejse C, Stryjewski ME, Abbate E, Hulme WF, Myers JL, Estevan R, Patillo SG, Olesen R, Tacconelli A, Sirugo G, Gilbert JR, Hamilton CD, Scott WK. Variants in toll-like receptors 2 and 9 influence susceptibility to pulmonary tuberculosis in Caucasians, African-Americans, and West Africans. Hum Genet;127:65-73. 2010 doi: 10.1007/s00439-009-0741-7. Epub 2009 Sep 22.

Zhang Y, Jiang T, Yang X, Xue Y, Wang C, Liu J, Zhang X, Chen Z, Zhao M, Li JC. Toll-like receptor -1, -2, and -6 polymorphisms and pulmonary tuberculosis susceptibility: a systematic review and meta-analysis. PLoS One. 2013 May 14;8(5):e63357. doi: 10.1371/journal.pone.0063357.

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Published

2014-10-31

How to Cite

Mohammed, S. M. H., Khalil, E. A. G., & Idris, F. M. (2014). </i>Toll-Like Receptor-2 </i>Gene <i>[TLR2]</i> Polymorphism and Susceptibility to <i>Mycobacteria Tuberculosis </i>Infections in Sudanese Patients. Asian Journal of Applied Sciences, 2(5). Retrieved from https://ajouronline.com/index.php/AJAS/article/view/1571

Issue

Vol. 2 No. 5 (2014): October 2014

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