Electromagnetic Therapy Control the Effect of Bacteria on Liver Tissue: Histopathological, Histochemical and Immunohistochemical Studies
Keywords:
electromagnetic waves, bacteria, apoptosis, collagen, caspase3, liverAbstract
Staphylococcus aureus (St. au.) is responsible for many human disorders including food poisoning, soft tissue infection, pneumonia and osteomylitis. The primary objective of this study is to reveal the role of electromagnetic waves (EMW) in the amelioration of the changes induced by (St. au.) infection either in rat liver tissue or its peripheral blood smears. 25 male rats were divided into 3 groups (G). G1: 5 control rats, G2: 10 experimental rats that were orally infected with St. au. by 108 cfu/ml, while G3: 10 experimental rats exposed after the incubation period (5days) to 0.8 Hz square magnetic pulses for 75 minutes. Our results revealed that some alterations included dilation of bile ducts, congestion of blood vessels; area of steatosis, increased infiltrated inflammatory cells, Kupffer cells inflammation and increased number of different apoptotic forms. Also abnormal shapes of RBCs, irregular shapes of monocytes, increased number of the pathogen and engulfed pathogen inside neutrophils were noticed. The intensity of collagen fibers was increased particularly at area of inflammation, around large vessels as well as sinusoids. The positivity of caspase 3 increased to be strong as compared with G1. Respecting G3, rats treated with EMW showed regression which was illustrated as reduced congestion of blood vessels, inflammation and steatosis, as well as reduction in apoptotic figures. The reappearance of normal biconcave RBCs and different types of WBCs was noticed except that there were no neutrophils. In addition, monocytes have delicate chromatin and there was no pathogen in the films, and the distribution of collagen fibers was similar to that of G1. Also the positivity of caspase 3 was more or less like that of G1. In conclusion: The noticed fast recovery of infected rats after their exposure to EMW in the present study opens a new avenue in the treatment of bacterial infection with dangerous strains like St.au. We expect that in the following years more approaches based on control of the different forms of cell death via EMW will enter the clinical practice.
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