Electromagnetic Therapy Control the Effect of Bacteria on Liver Tissue: Histopathological, Histochemical and Immunohistochemical Studies


  • Safia Mohammed Hassan Department of Histochemistry and Cell Biology, Medical Research Institute, Alexandria University


electromagnetic waves, bacteria, apoptosis, collagen, caspase3, liver


Staphylococcus aureus (St. au.) is responsible for many human disorders including food poisoning, soft tissue infection, pneumonia and osteomylitis. The primary objective of this study is to reveal the role of electromagnetic waves (EMW) in the amelioration of the changes induced by (St. au.) infection either in rat liver tissue or its peripheral blood smears. 25 male rats were divided into 3 groups (G). G1: 5 control rats, G2: 10 experimental rats that were orally infected with St. au. by 108 cfu/ml, while G3: 10 experimental rats exposed after the incubation period (5days) to 0.8 Hz square magnetic pulses for 75 minutes. Our results revealed that some alterations included dilation of bile ducts, congestion of blood vessels; area of steatosis, increased infiltrated inflammatory cells, Kupffer cells inflammation and increased number of different apoptotic forms. Also abnormal shapes of RBCs, irregular shapes of monocytes, increased number of the pathogen and engulfed pathogen inside neutrophils were noticed. The intensity of collagen fibers was increased particularly at area of inflammation, around large vessels as well as sinusoids. The positivity of caspase 3 increased to be strong as compared with G1. Respecting G3, rats treated with EMW showed regression which was illustrated as reduced congestion of blood vessels, inflammation and steatosis, as well as reduction in apoptotic figures. The reappearance of normal biconcave RBCs and different types of WBCs was noticed except that there were no neutrophils. In addition, monocytes have delicate chromatin and there was no pathogen in the films, and the distribution of collagen fibers was similar to that of G1. Also the positivity of caspase 3 was more or less like that of G1. In conclusion: The noticed fast recovery of infected rats after their exposure to EMW in the present study opens a new avenue in the treatment of bacterial infection with dangerous strains like St.au. We expect that in the following years more approaches based on control of the different forms of cell death via EMW will enter the clinical practice.



Balmori A. Electromagnetic pollution from phone masts. Effects on wildlife. Pathophysiology 2009; 16: 191-9.

Wain J, Kidgell C. The emergence of multidrug resistance to antimicrobial agents for the treatment of thyroid fever. Transactions of the Royal Society of Tropical Medicine and Hygiene 2004; 98: 423-30.

Brusick D, Albertini R, McRee d, Peterson D. Genotoxicity of radiofrequency radiation. Epert Panel. Environ Med Mutagen 1998; 32 (1): 1-16.

Wiakman G, Julian L, Olson MF. How apoptotic cells aid in the removal of their own cold dead bodies. Cell Death differ 2012; 19: 735-42.

Al-Nakeeb NKM. The pathogenesis of experimental infection by Staphylococcu aureus in rabbits. Kufa J Vet Med Sci 2011; 2(2): 127-40.

Ali FM, Gawish AM, Osman MBS, Abdelbacki AM, El-Sharkawy AH. Control of salmonella activity in rats by pulsed ELF magnetic field (in vivo study). J Inter Dent Med Res 2012; 5(2): 129-35.

WHO. Blood Safety and Clinical Technology: Guidelines on Standard Operating Procedures for Haematology, Preparation and Staining of Blood Films (Chapter 11), (Last update: 27 April 2006)

- Suvik A, Effendy AW. The use of modified Masson’s Trichrome Staining in collagen evaluation in wound healing study. Malaysian J Vet Res 2012; 3: 39-47.

- Barrett KL, Willingham J, Garvin AJ, Willingham MC. Advances in cytochemical methods for detection of apoptosis. J Histochem Cytochem 2001; 49: 821–32.

- Vihayalami, Leal BZ, Szilagyi M, Prihoda TJ, Meltz ML. Primary DNA damage in human blood lymphocytes exposed in vitro to 2450 MHz radiofrequency radiation. Radiat Res 2009; 153 (4): 479-86.

- Tice RR, Hook GG, Donnor M, McReee DI, Guy AW. Genotoxicity of radiofrequency signals. I. Investigation of GNA damage and micronuclei in cultured human blood cells. Bioelectromag 2002, 23 (2): 113-26.

- Tian F, Nakahara T, Yoshida H, Honda N, Hirose H, Miyakoshi J. Exposure to power frequency magnetic fields suppression X-ray-induced apoptosis transiently in Ku80-defieient xrs5 cells. Biochem Biophys Res Comm 2002; 292 (2): 355-61.

- Giladi M, Pori Y, Blott A, Wasserman Y, Kirson E, Dekel E, Palti Y. Microbial growth inhibition by alternating electrical fields. Antimicrobial Agent Chemotherapy 2008; 52 (10): 3517-22.

- Ruiz-Gomez , Sendra-Portero F, Martinez-Marillo M. Effect of 2.45 mt sinusoidal 50Hz magnetic field on saccharomyces cervisiae strain deficient in DNA strand breaks repair. Int J Rad Biol 2010; 86: 602-11.

- Ayse I, Burake A, Zafer A, Dilek A, Nilufer A, Tangul S. Effect of extremely low frequency electromagnetic fields on growth rate and morphology of bacteria. Int J Rad Biol 2011; 88: 1-8.

- Esen M, Schreiner B, Jendrossek V, Lang F, Fassbender K, Grassne H, Gulbins E. Mechanisms of Staphylococcus aureus induced apoptosis in human endothelial cells. Apoptosis 2001; 6: 431-9.

- Atasoy A, Sevim Y, Kayor I, Yilmaz M, Durmus A, Sonmwz M, Ozdemir F, Ovali E. The effect of electromagnetic fields on peripheral blood mononuclear cell in vitro. Bratisl Lek Listy 2009; 110 (9): 526-9.

- Akan Z, Aksu B, Tulunay A, Bilsel S, Inhan-Garip A. Extremely low-frequency electromagnetic fields affect the immune response of monocyte-derived macrophages to pathogens. Biolectromagnetics 2010; 31: 603-12.

- Coskun S, Balabanli B, Canseven A, Seyhan N. Effects of continuous and intermittent magnetic fields on oxidative parameters. In vivo. Neurodermical Res 2009; 34: 238-43.

- Yin H, Li X, Hu S, Liu T, Yuan B, Ni Q, Lan F, Luo X, Gu H, Zheng F. IL-33 promotes Staphylococcus aureus infected wound healing in mice. Int Immunopharmacol 2013; 17 (2): 432-8.

- Olsson M, Zhivotovsky B. Caspases and cancer. Cell Death Differ 2011; 18: 1441-9.

- Ai X, Butts B, Vora K, Li W, Tache-Talmadge C, Fridman A, Mehmet H. Generation and characterization of antibodies specific for caspase-cleaved neo epitopes: a novel approach. Cell Death Differ 2011; 2: 205.




How to Cite

Hassan, S. M. (2014). Electromagnetic Therapy Control the Effect of Bacteria on Liver Tissue: Histopathological, Histochemical and Immunohistochemical Studies. Asian Journal of Applied Sciences, 2(1). Retrieved from https://ajouronline.com/index.php/AJAS/article/view/818