Ameliorative Effect of Propolis Extract on Hepatotoxicity Induced by Methotrexate in Mice
Keywords:
Propolis, Methotrexate, liver, antioxidants, cytokinesAbstract
Propolis, rich in natural antioxidant flavonoids. It is produced by honey bee (Apis melifera ligutica). Our study aimed to evaluate the ameliorative effects of Propolis water extract on liver toxicity induced by methotrexate. The study used thirty-five male albino mice randomly divided into five groups. The first is control (C); the second, Methotrexate-induced hepatotoxicity (MTX, single dose 20 mg/kg IP); Third, Propolis water extract (PWE, 100 mg/kg PO for 7 days) and both fourth and fifth groups are Proplis Before/After Methotrexate, PBM and PAM; respectively). Serum aspartate aminotransferase (ASTA), alanine aminotransferase (ALTA), alkaline phosphatase (ALP), γ-glutamyle transferase (γ-GT), C-reactive protein (CRP) and hepatic histopathology estimated the protective effect of Propolis against the liver injury induced by MTX. Catalase (CAT) and superoxide dismutase (SOD) evaluated the antioxidant activities in liver tissue. In additions, the immune efficacy of Propolis were determined by liver interleukins levels (IL4, IL10, IL1 and IL6). The results revealed that Propolis treatment before injection of Methotrexate have significant hepatoprotective, antioxidant and enhancing immune liver response compared to PAM groups. The study, assumes in line with the fact that Propolis is a natural, low cost and nontoxic product possesses ameliorative activities against hepatic alterations induced by MTX.
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