Antibacteria Activity of Shewanella and Pseudomonas as Endophytic Bacteria from the Root of Ageratum conyzoides L.
Keywords:
Ageratum conyzoides, Antibacteria, Endophytic bacteriaAbstract
Most of plants have endophytic bacteria that are able to synthesize some compounds that have  biological activities as antibacteria. Four isolates of endophytic bacteria from root of Ageratum conyzoides was isolated and identified. The result of the identification of their morphology and biochemical activity shows that those bacteria are from Shewanella and Pseudomonas. The purpose of the research is to obtain crude extract of supernatant of endophytic bacteria in root A. conyzoides that performs antibacteria activity and to discover the identity of potential antibacteria compounds from the crude extract. The extraction of supernatant was done by using ethyl acetate. Then, antibacteria of each crude extract was tested on three pathogenic bacteria; i.e. Eschericia coli, Pseudomonas aeruginosa, and Staphylococus aureus. The result of the test indicates that those crude extracts perform antibacteria activity. The crude extract of supernatant of Shewanella presents the highest level with zone of inhibition of 11.92 ± 1.24 mm (E. coli), 12.85 ± 0.32 mm (P. aeruginosa), and 15.13 ± 0.88 mm (S. aureus). The result of the test on crude-extract compound by using GC-MS discovers the existence of two active compounds; those are 2-amino-3-quinoline carbonitrile and boric acid. The result of the research asserts that endophytic bacteria from root A. conyzoides demonstrate the potential to be a promising source of antibacteria compound.
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