Copper Tolerance and Copper Accumulation of Culturable Endophytic Yeasts of Phragmites Australis Cav. (Trin) ex Steud. From the Mine Tailings Pond in Mankayan, Benguet, Philippines
Keywords:
Endophytic yeasts, copper tolerance, Phragmites australis, bioconcentration factor, ascorbate peroxidase, catalaseAbstract
Three endophytic yeasts of Phragmites australis Cav. (Trin) ex Steud. from the tailings pond 5 of Lepanto Consolidated Mining Co. in Mankayan, Benguet, Philippines were investigated for their capacity to tolerate and accumulate copper. Two of the isolates were successfully identified through sequence homology of their ITS genes. All isolates were able to tolerate 500ppm Cu concentrations. The isolate that sequestered the most copper was D. hansenii Yph 4 at 9.38 µg/mg. The isolate that had the best bioconcentration factor was Candida parapsilosis Yph5 at 74.09%. Ascorbate peroxidase and catalase assays of yeasts grown on 50 ppm copper were done. The highest producer of APX was C. parapsilosis Yph5. On the other hand, the best catalase producer was the unidentified yeast Yph3.
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