Effect of Gonadotrophic Hormones and Insulin-like Growth Factor I (IGF-i) on Maturation of Mammalian Oocytes in vitro

Authors

  • Abd El Wahab El Ghareeb Zoology Department, Faculty of science, Cairo University, Cairo
  • Amr El Ahwany Andrology Department, Faculty of Medicine, Cairo University
  • Mohamed Khamis IVF Centre, Nile Badrawi Hospital, Cairo University
  • Tawfik Al Aref Department of Zoology, Faculty of Science, Cairo University

Abstract

Mammalian oocyte in vitro maturation (IVM) is a fundamental technique in human-assisted reproduction clinic and reproductive biology research, which can provide high quality oocytes for gamete and embryo engineering. However, IVM remains a challenge in mammalian species, and has only been achieved with limited degrees of success. A number of hormones and growth factors have been reported to affect the in vitro maturationof oocytes. In the present study, we have investigated the effects of gonadotrophic hormones and insulin-like growth factor 1(IGF-I) on the in vitro maturation of rabbit oocytes.

Our study was carried out to investigate the role of adding follicle-stimulating hormone (FSH), luteinizing hormone (LH) or the combination of both hormones with Insulin-like growth factor 1 (IGF-I) on the IVM of rabbit oocytes, as rabbit is considered as an important model system for human reproduction. Unstimulated ovaries of female rabbits were encountered. Oocytes were collected using slicing technique. The cumulus oocyte complexes (COCs) containing immature oocytes at germinal vesicle (G.V) stage were counted, washed, and then cultured in different concentrations of FSH, LH, combination of both FSH and LH with Insulin-like growth factor 1 (IGF-I) .The oocyte maturation was examined and our study revealed that the addition of certain concentrations of hormone combinations (FSH + LH) with Insulin-like growth factor 1 (IGF-I) could significantly improve the IVM and increase the percentage of oocytes reaching MII phase.

 

References

• Adashi, EY. andHsueh, AJW. (1982): Estrogens augment the stimulation of ovarian aromatase activity by follicle-stimulating hormone in culture rat granulosa cells. J. Biol. Chem., 257: 6077-6083.

• Arunakumari, G.; Vagdevi, R.; Rao, B.S.; Naik, B.R.; Naidu, K.S.; Kumar, R.V.S. and Rao, V.H.(2007): Effect of hormones and growth factors on in vitro development of sheep preantral follicles. Small Rumin. Res., 70: 93-100.

• Bavister, B.D.; Rose-Hellekant, T.A. and Pinyopummintr, T. (1992): Development of in vitro matured/in vitro fertilized bovine embryos into morulae and blastocysts in defined culture media. Theriogenology, 37: 127-146.

• Bilodeau-Goeseels, S. and Panich, P. (2002): Effects of oocyte quality on development and transcriptional activity in early bovine embryos. Anim. Reprod. Sci., 71: 143-155.

• Brackett, B.G.; Younis, A.I. and Fayrer-Hosken, R.A. (1989): Enhanced viability after in vitro fertilization of bovine oocytes matured in vitro with high concentrations of luteinizing hormone. Fertil.Steril., 52: 319-324.

• Byskov, A.G.; Andersen, C.Y.; Hossaini, A. and Guoliang, X. (1997): Cumulus cells of oocyte-cumulus complexes secrete a meiosis-activating substance when stimulated with FSH. Mol. Reprod. Dev., 46: 296-305.

• Cha, K.Y. and Chian, R.C. (1998): Maturation in vitro of immature human oocytes for clinical use. Hum. Reprod. Update, 4:103–120.

• Chauhan, M.S.; Katiyar, P.K. and Madan, M.L. (1997): in vitro production of blastocyst in goats, sheep and buffaloes. Indian J. Anim. Sci., 67: 394-396.

• Chen, L.; Wert, S.E.; Hendrix, E.M.; Russell, P.T.; Cannon, M. and Larsen, W.J. (1990): Hyaluronic acid synthesis and gap junction endocytosis are necessary for normal expansion of the cumulus mass. Mol. Reprod. Dev., 26:236-247.

• Chian, R.C.; Niwa, K. and Sirard, M.A. (1994): Effect of cumulus cells on male pronuclear formation and subsequent early development of bovine oocytes in vitro. Theriogenology, 41: 1499-1508.

• Choi, Y.H.; Carnevale, E.M.; Seidel, G.E. Jr. and Squires, E.L. (2001): Effects of gonadotropins on bovine oocytes matured in TCM-199 Theriogenology, 56: 661-670.

• David, K. Gardner (2006):In vitro Fertilization Book. Informa Healthcare, NY, 127-140

• De Felici, M. and Siracusa, G. (1982): Spontaneous hardening of the zonapellucida of mouse oocytes during in vitro culture. Gamete Res., 6: 107-113.

• Dekel and Scherizly I (1985) Epidermal growth factor induces maturation of rat follicle-enclosed oocytes Endocrinology 116: 512—516

• Downs SM (1989) Specificity of epidermal growth factor action on maturation of the murine oocyte and the cumulus oophorus in vitro Biology of Reproduction 41: 371-379

• Edwards RG (1965) Maturation in vitro of mouse, sheep, cow, pig, rhesus monkey and human ovarian oocytes Nature 208: 349—351

• Elvin, J.A.; Clark, A.T.; Wang, P.; Wolfman, N.M. and Matzuk, M.M. (1999): Paracrine actions of growth differentiation factor-9 in the mammalian ovary. Mol. Endocrinol., 13: 10351048.

• Engmann, L.; Maconochie, N.; Bekir, J.S.; Jacobs, H.S. and Tan, S.L. (1999): Cumulative probability of clinical pregnancy and live birth after a multiple cycle IVF package: a more realistic assessment of overall and age-specific success rates. Br. J. Obstet. Gynecol., 106: 165–170.

• Eppig, J.J.; Schroeder, A.C. and O’Brien, M.J. (1992). Developmental capacity of mouse oocytes matured in vitro: effects of gonadotrophic stimulation, follicular origin and oocyte size. J. Reprod. Fertil., 95:119-127.

• Fukushima, M. and Fukui, Y. (1985): Effects of gonadotropins and steroids on the subsequent fertilizability of extrafollicular bovine oocytes cultured in vitro. Anim. Reprod.Sci., 9: 323- 332.

• Ge, L.; Han, D.; Lan, G.C.; Zhou, P.; Liu, Y.; Zhang, X.; Sui, H.S. and Tan, J.H. (2008): Factors affecting the in vitro action of cumulus cells on the maturing mouse oocytes. Mol. Reprod. Dev., 75:136-142.

• Gilchrist, R.B. and Thompson, J. G. (2007): Oocyte maturation: Emerging concepts and technologies to improve developmental potential in vitro. Theriogenology, 67: 6-15.

• Goldenberg RL, Vaitukaitis JL and Ross GT. (1972): Estrogen and follicle stimulating hormone interactions on follicle growth in rats. Endocrinology, 90:1492-1498.

• Gospodarowicz D and Bialecki H (1979) Fibroblast and epidermal growth factors are mitogenic agents for cultured granulosa cells of rodent, porcine and human origin Endocrinology 104: 757—764

• Hardy, K.; Wright, C.S.; Franks, S. and Winston, R.M. (2000):In vitro maturation of oocytes. Br. Med. Bull., 56:588–602.

• Henderson, K.M.; McNeilly, A.S. and Swanston, I.A. (1982): Gonadotropin and steroid concentrations in bovine follicular fluid and their relationship to follicle size. J. Reprod. Fertil., 65: 467-473.

• Hernandez ER, Resnick CE, Svoboda ME, Van Wyk JJ, Payne DW and Adashi EY (1988) Somatomedin-C/insulin-like growth factor I as an enhancer of androgen biosynthesis by cultured rat ovarian cells Endocrinology 122:1603-1612

• Hillier, S.G.; Wickings, E.J.; Saunders, P.T., et al. (1989): Control of inhibin production by primate granulose cells. J. Endocrinol., 123: 65-73.

• Hsueh AJW, Welsh TH and Jones BPC (1981) Inhibition of ovarian and testicular steroidogenesis by epidermal growth factor Endocrinology 108:2002-2004

• Hsu C and Hammond JM (1987) Gonadotropins and estradiol stimulate immunoreactive insulin-like growth factor-I production by porcine cells in vitro Endocrinology 120: 198-207

• Jin-Hua, L.; Jun-Zuo, W.; Hui-Li, W.; Hong-Shu, S.; Yong, L.; Jing-Jing, L.; Bo, L. and Jing-He, T. (2010): Damaging effect of cumulus denudation on rabbit oocytes. Fertil.Steril., 93:1567–73.

• Kharche, S.D.; Goel, A.K.; Jindal, S.K. and Sinha, N.K. (2006): Technical note: In vitro maturation of caprine oocytes in different concentration of estrous goat serum. Small Rumin. Res., 64: 186-189.

• Kito, S. and Bavister, B.D. (1997): Male pronuclear formation and early embryonic development of hamster oocytes matured in vitrowith gonadotrophins, amino acids and cysteamine. J. Reprod. Fertil., 110: 35-46.

• Lonergan, P.; Rizos, D.; Gutirez, A.; Fair, T. and Boland, M.P. (2003): Oocyte and embryo quality: effect of origin, culture conditions and gene expression patterns. Reprod. Domest.Anim., 38: 259-267.

• Lorenzo, P.L.; Rebollar, P.G.; Illera, M.J.; Illera, J.C.; Illera, M. and Alvarino, J.M.R. (1996).Stimulatory effect of insulin-like growth factor I and epidermal growth factor on the maturation of rabbit oocytes in vitro. J. Reprod. Fertil., 107: 109-l 17.

• Madan, M.L.; Chauhan, M.S.; Singla, S.K. and Manik, R.S. (1994): Pregnancies established from water buffalo (Bubalusbubalis) blastocysts derived from in vitro matured, in vitro fertilized and co-cultured with cumulus and oviductal cells. Theriogenology, 42: 591-600.

• Mattioli, M.; Bacci, M.L.; Galeati, G. and Seren, E. (1991): Effect of LH and FSH on the maturation of pig oocytes in vitro. Theriogenology, 36: 95- 105.

• May JV, Buck PA and Shomberg DW (1987)Epidermal growth factor enhances I25iodo-follicle stimulating hormone binding by cultured porcine granulosa cells Endocrinology 120: 2413-2420

• Moor, R.M. and Trounson, A.O. (1977): Hormonal and follicular factors affecting maturation of sheep oocytes in vitro and their subsequent developmental capacity. J. Reprod. Fertil., 49: 101-109.

• Mori, T.; Amano, T. and Shimizu, H. (2000): Roles of gap junctional communication of cumulus cells in cytoplasmic maturation of porcine oocytescultured in vitro. Biol. Reprod., 62: 913-919.

• Pawshe, C.H.; Palanisamy, A.; Taneja, M.; Jain, S.K. and Totey, S.M. (1996): Comparison of various maturation treatments on in vitro maturation of goat oocytes and their early embryonic development and cell numbers. Theriogenology, 46: 971-982.

• Peng, X.R.; Hsueh, A.J.W.; Lapolt, P.S.; Bjersing, L. and Ny, T. (1991): Localization of luteinizing hormone receptor messenger ribonucleic acid expression in ovarian cell types during follicle development and ovulation. Endocrinology, 129: 3200-3207.

• Pincus, G. and Enzmann, E.V. (1935): The comparative behavior of mammalian eggs in vivo and in vitro. I. The activation of ovarian eggs. J. Exp. Med., 62: 655–675.

• Richards, J.S. (1975): Estradiol receptor content in rat granulosa cells during follicular development: modification by estradiol and gonadotropins. Endocrinology, 97: 1174-1184.

• Romero-Arredondo, A. and Seidel, G. Jr. (1996): Effects of follicular fluid during in vitro maturation of bovine oocytes on in vitro fertilization and early embryonic development. Biol. Reprod., 55:1012-1016.

• Saeki, K.; Hoshi, M.; Leibfried-Rutledge, M.L. and First, N.L. (1991):In vitro fertilization and development of bovine oocytes matured in serum-free medium. Biol. Reprod., 44:256-260.

• Shirazi, A.; Shams-Esfandabadi, N.; Hosseini, S.M. and Karimi, I. (2007): The presence of cumulus cells on nuclear maturation of sheep oocytes during in vitro maturation. Small Rumin. Res., 68: 291-295.

• Tajik, P. and Shams-Esfandabadi, N. (2003):In vitro maturation of caprine oocytes in different culture media. Small Rumin.Res., 47: 155- 158.

• Tamilmani, G.; Rao, B.S.; Vegdevi, R.; Amarnath, D.; Naik, B.R.; Mutharao, M. and Rao, V.H. (2005): Nuclear maturation of bovine oocytes in cultured preantral follicles. Small Rumin. Res., 60: 295-305.

• Tan, S.L.; Child, T.J. and Gulekli, B. (2002):In vitro maturation and fertilization of oocytes from unstimulated ovaries: predicting the number of immature oocytes retrieved by early follicular phase ultrasonography. Am. J. Obstet. Gynecol., 186: 684–689.

• Tao, Y.; Xie, H. R.; Hong, H. Y.; Chen, X. F.; Jang, J.; Xia, G. L., (2005): Effects of nitric oxide synthase inhibitors on porcine oocyte meiotic maturation. Zygote, 13: 1–9.

• Tapanainen, J.; McCamant, S.; Orava, M.; Ronnberg, L.; Markianen, H. and Jaffe, R.B. (1991).Regulation of steroid and steroid sulfate production and aromatase activity in cultured human granulose-luteal cells.J. Steroid Biochem. Mol. Biol., 39: 19-25.

• Totey, S.M.; Pawshe, C.H .and Singh, G.P. (1993):In vitro maturation and fertilization of buffalo oocytes (Bubalusbubalus): effects of media, hormones and sera. Theriogenology, 9: 153-171.

• Totey, S.M.; Singh, G.; Taneja, M.; Pawshe, C.H. and Talwar, G.P. (1992):In vitro maturation, fertilization and development of follicular oocytes from buffalo (Bubalusbubalus). J. Reprod. Fertil., 95: 595- 607.

• Tsafriri, A.; Cao. X.; Ashkenazi, H.; Mottola, S.; Popliker M. and Pomerantz, S.H. (2005): Resumption of oocyte meiosis in mammals: on models, meiosis activating sterols, steroids and EGFlike factors. Mol. Cell. Endocrinol., 234(1-2): 37- 45.

• Vanderhyden, B.C. and Armstrong, D.T. (1989).Role of cumulus cells and serum on the in vitro maturation, fertilization, and subsequent development of rat oocytes.Mol. Reprod. Dev., 40:720-728.

• Veldhuis JD, Rodgers RJ and Furlanetto RW (1986) Synergistic actions of estradiol and the insulin-like growth factor somatomedin-C on swine ovarian (granulosa) cells Endocrinology 119: 530—538

• Webb, R.J.; Bains. H.; Cruttwell, C. and Caroll, J. (2002): Gap-junctional communication in mouse cumulus-oocyte complexe: implications for the mechanism of meiotic maturation. Reproduction, 123: 41-52.

• Westergaard LG, Andersen CY and Byskov AG (1990) Epidermal growth factor (EGF) in small antral ovarian follicles of pregnant women Journal of Endocrinology 127: 363-367

• Yadave, B.R.; Katiyar, P.K.; Chauhen; M.S. and Maden, M.L. (1997): Chromosome configuration during in vitro maturation of goat, sheep and buffalo oocytes. Theriogenology, 47: 943- 951.

• Yamauchi, N. and Nagai, T. (1999): Male pronuclear formation in denuded porcine oocytes after in vitro maturation in the presence of cysteamine. Biol. Reprod., 61: 828-833.

• Yoshida, M.; Ishizaki, Y.; Kawagishi, H.; Bamba, K. and Kojima, Y. (1992): Effects of pig follicular fluid on maturation of pig oocytes in vitro and on their subsequent fertilizing and developmental capacity in vitro. J. Reprod. Fertil., 95:481-488.

Published

2016-06-16

Issue

Vol. 4 No. 3 (2016): June 2016

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How to Cite

Effect of Gonadotrophic Hormones and Insulin-like Growth Factor I (IGF-i) on Maturation of Mammalian Oocytes in vitro. (2016). Asian Journal of Applied Sciences, 4(3). https://ajouronline.com/index.php/AJAS/article/view/3777

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