Comparison of Particle Bombardment and Silicon Carbide Whisker Vortex Mixing Methods As Mediators For NMU Based Mutagenesis Agent on Aglaonema
Keywords:
Aglaonema, NMU, mutagenesis, variegated, particle bombardment, silicon carbideAbstract
Mutagenesis of cultured Aglaonema axillary buds on Murashige-Skoog medium was performed using mutagen Nitrosomethylurea (NMU). The process was performed with 2 different mediation methods: vacuum-gunpowder powered particle gun with tungsten microprojectile, and silicon carbide whisker added into direct mutagen chemical under utilization of vortex shaker mixing (vortexing) method. Particle bombardment approach was used under 3 different levels of diluted concentrations: 1, 10, 100, and 1000 times of dilution, while for vortexing only used 1000 times of dilution (10, 25, and 50 μM) under 1, 3, and 5 minutes Results of these 2 methods were production of green, pale green, white, and dead shoots. Applying of vortexing with silicone carbide provides optimum mutagenesis only in lower concentration as dead samples occurred in higher level of NMU concentration beyond 25 μM with lethal dosage of 75% (LD75) is 25 μM with 1 minute of duration. Using particle bombardment mediation for mutagenesis, wider range and diverged mutants were produced. Particle bombardment is concluded to be safer mediation method even in least concentration of NMU dilution. Vortexing mediation offered optimization for lower maximum range of concentration, 25 μM in less than 3 minutes of mixing duration.
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