Cell Development during Somatic Embryogenesis in Lithospermum erythrorhizon Observed by Scanning Electron Microscopy
Keywords:
Lithospermum erythrorhizon, somatic embryogenesis, morphology, scanning electron microscopyAbstract
Scanning electron microscopy observation could be used to describe cell development during somatic embryogenesis in Lithospermum erythrorhizon. The purpose of this study is to provide numbers of important morphological information in order to understand the embryogenesis of this plant. Proembryo was composed of small round cells. Proliferating embryogenic cell (PEC) was composed of small round cells in the center, surrounded by slightly elongated cells at the periphery. Globular somatic embryo composed of small round cells. Its surface was covered by extracellular material. Â Heart somatic embryo was composed of small round cells. Slightly elongated cells in pre-root apex and round cells in pre-shoot apex of early torpedo somatic embryo showing polarization of somatic embryo. In late torpedo somatic embryos, pre-root apex became firm and pre-shoot apex still consisted of round cells.
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