Simple Micropropagation by Indirect Somatic Embryogenesis in Sugarcane (Saccharum officinarum L.)

Authors

  • Totik Sri Mariani School of Life Sciences and Technology, Bandung Institute of Technology,
  • Sony Suhandono
  • Dini Ermavitalini
  • Mr. Ihsan
  • Tita Puspita

Keywords:

Sugarcane, 2, 4-D, somatic embryo, plantlet

Abstract

The simple method for producing sugarcane somatic embryos was developed by reducing concentration of 2,4-D. Callus of sugarcane was maintained on MS medium supplemented by 3 ppm of 2,4-D (D3). The callus surface changed into white opaque colour  (white nodule) when the concentration of 2,4-D was decreased to 1.5 ppm (D1.5). Thereafter, the somatic embryos developed from the white nodule. There are various developmental stage of sugarcane somatic embryos on D1.5 medium, namely globular, early scutellar, late scutellar, coleoptilar embryos. When the 2,4-D was omitted from the medium (D0), the coleoptilar somatic embryos germinated , formed shoot and root. The germinated somatic embryos were growing well forming plantlet. This simple method offers a convenient tool for micropropagation of sugarcane.

 

Author Biography

Totik Sri Mariani, School of Life Sciences and Technology, Bandung Institute of Technology,

School of Life Sciences and Technology

Rank :A

References

• Brisibe, E.A., Nishioka, D., Miyake, H., Taniguchi, T., and Maeda, E. 1993. Developmental electron microscopy and histochemistry of somatic embryo differentiation in sugarcane. Plant Science. 89: 85-92

• Brisibe, E.A., Miyake, H., Taniguchi, T, Maeda, E. 1994. Regulation of somatic embryogenesis in long-term callus cultures of sugarcane (Saccharum officinarum L.). New Phytologist. 126 (2) : 301-307

• Desai, N.S., Joseph, D., Suprasanna, P., Bapat, V.A. 2006. Study of elemental variations during somatic embryogenesis in sugarcane using photon induced X-ray probe. Nuclear instruments and Methods in Physics Research B 252:299-302

• Mariani, T.S., Miyake, H. and Takeoka, Y. 1998. Changes in surface structure during direct somatic embryogenesis in rice scutellum observed by scanning electron microscopy. Plant Production Science. 1(3): 223-231

• Purnaning, A.S., 2006. Pengaruh penambahan glutamine pada tahap maturasi terhadap germinasi dan konversi plantlet embrio somatic kelapa sawit (Elaeis guineensis Jacq.). Scription. School of Life Sciences and Technology, Bandung Institute of Technology, Indonesia.

• Rodriguez, S., Mondejar, C., Ramos, M.E., Diaz, E., Maribona, R., Ancheta, O. 1995. Sugarcane somatic embryogenesis: a scanning electron microscopy study. Tissue& Cell. 28(2) : 149-154

• Williams, E.G. and Maheswaran, G. 1986. Somatic embryogenesis: Factors influencing coordinated behavior of cells as an embryogenic group. Ann. Bot. 57: 443-462.

Downloads

Published

2014-04-22

How to Cite

Mariani, T. S., Suhandono, S., Ermavitalini, D., Ihsan, M., & Puspita, T. (2014). Simple Micropropagation by Indirect Somatic Embryogenesis in Sugarcane (Saccharum officinarum L.). Asian Journal of Applied Sciences, 2(2). Retrieved from https://ajouronline.com/index.php/AJAS/article/view/1012

Issue

Section

Articles

Most read articles by the same author(s)