Simple Micropropagation by Indirect Somatic Embryogenesis in Sugarcane (Saccharum officinarum L.)
Keywords:
Sugarcane, 2, 4-D, somatic embryo, plantletAbstract
The simple method for producing sugarcane somatic embryos was developed by reducing concentration of 2,4-D. Callus of sugarcane was maintained on MS medium supplemented by 3 ppm of 2,4-D (D3). The callus surface changed into white opaque colour (white nodule) when the concentration of 2,4-D was decreased to 1.5 ppm (D1.5). Thereafter, the somatic embryos developed from the white nodule. There are various developmental stage of sugarcane somatic embryos on D1.5 medium, namely globular, early scutellar, late scutellar, coleoptilar embryos. When the 2,4-D was omitted from the medium (D0), the coleoptilar somatic embryos germinated , formed shoot and root. The germinated somatic embryos were growing well forming plantlet. This simple method offers a convenient tool for micropropagation of sugarcane.
Â
References
• Brisibe, E.A., Nishioka, D., Miyake, H., Taniguchi, T., and Maeda, E. 1993. Developmental electron microscopy and histochemistry of somatic embryo differentiation in sugarcane. Plant Science. 89: 85-92
• Brisibe, E.A., Miyake, H., Taniguchi, T, Maeda, E. 1994. Regulation of somatic embryogenesis in long-term callus cultures of sugarcane (Saccharum officinarum L.). New Phytologist. 126 (2) : 301-307
• Desai, N.S., Joseph, D., Suprasanna, P., Bapat, V.A. 2006. Study of elemental variations during somatic embryogenesis in sugarcane using photon induced X-ray probe. Nuclear instruments and Methods in Physics Research B 252:299-302
• Mariani, T.S., Miyake, H. and Takeoka, Y. 1998. Changes in surface structure during direct somatic embryogenesis in rice scutellum observed by scanning electron microscopy. Plant Production Science. 1(3): 223-231
• Purnaning, A.S., 2006. Pengaruh penambahan glutamine pada tahap maturasi terhadap germinasi dan konversi plantlet embrio somatic kelapa sawit (Elaeis guineensis Jacq.). Scription. School of Life Sciences and Technology, Bandung Institute of Technology, Indonesia.
• Rodriguez, S., Mondejar, C., Ramos, M.E., Diaz, E., Maribona, R., Ancheta, O. 1995. Sugarcane somatic embryogenesis: a scanning electron microscopy study. Tissue& Cell. 28(2) : 149-154
• Williams, E.G. and Maheswaran, G. 1986. Somatic embryogenesis: Factors influencing coordinated behavior of cells as an embryogenic group. Ann. Bot. 57: 443-462.
Downloads
Published
How to Cite
Issue
Section
License
- Papers must be submitted on the understanding that they have not been published elsewhere (except in the form of an abstract or as part of a published lecture, review, or thesis) and are not currently under consideration by another journal published by any other publisher.
- It is also the authors responsibility to ensure that the articles emanating from a particular source are submitted with the necessary approval.
- The authors warrant that the paper is original and that he/she is the author of the paper, except for material that is clearly identified as to its original source, with permission notices from the copyright owners where required.
- The authors ensure that all the references carefully and they are accurate in the text as well as in the list of references (and vice versa).
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Attribution-NonCommercial 4.0 International that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
- The journal/publisher is not responsible for subsequent uses of the work. It is the author's responsibility to bring an infringement action if so desired by the author.