Diversity of Endophytic Bacteria Related to Antibacteria Activity Isolated from Vetiveria zizanioides L. (WT)

Authors

  • Any Fitriani Indonesia University of Education
  • Rita Shintawati
  • Peristiwati, Filda Husnawati

Keywords:

endophytic bacteria, antibacteria, Vetiveria zizanioides

Abstract

Diversity of endophytic bacteria isolated from Vetiveria zizanioides L. (WT) has been done. Endophytic bacteria is bacteria which living in the cell or intercellular space without cause desease to host. V. zizanioides (WT) is the medicinal plant as fragrance, antibacteria, antifungi. Endophytic bacteria lived in roots cell and produced secondary metabolites. The aim of the research is  isolating bacteria from the roots, characterizing of morphology and biochemistry, evaluating antimicrobe activity, and identifying the microbes. Endophytic bacteria from the roots of V. zizanioides has been isolated and got 22 different isolates. Based on Gram staining, they have 20 baccil and 2 coccus, 17 Gram negative and 5 Gram positive. Biochemistry analysis was done and variability results. Antibacteria analysis showed that 7 isolates potentially produced secondary metabolites as antibacteria.

References

Ratha M, “Screening of phytochemical and antibacterial activity of Hemidesmus indicus (L.) and Vetiveria zizanoides (L)â€, European Journal of Experimental Biology, vol. 2, no. 2, pp. 363-368. 2012.

Nantachit K, “Antimicrobial Activity of Alkaloid from Roots of Vetiveria zizanoides L. Nash ex Smallâ€, Thai Pharm Health Science Journa, vol.5, no. 2, pp. 99 – 102. 2010.

Pimentel M, “The Use of Endophytes to Obtain Bioactive Compounds and Their Application in Biotransformation Processâ€, SAGE-Hindawi Access to Research Biotechnology Research International, 2012.

Strobel G, Daisy B, “Bioprospecting for Microbial Endophytes and Their Natural Productsâ€, Microbiology and Molecular Biology Reviews, vol. 67, no. 4, pp. 491–502, 2003.

Fitriani A, Ihsan F, Hamdiyati Y, Maemunah, “Antibacteria Activity of Shewanella and Pseudomonas as Endophytic Bacteria from the Root of Ageratum conyzoides Lâ€, Asian Journal of Applied Sciences (ISSN: 2321 – 0893) Vol. 03 – Issue 03, June 2015.

Radji M, “Peranan Bioteknologi dan Mikroba Endofit dalam Pengembangan Obat Herbalâ€, vol. II, no. 3, pp. 1693-9883, 2005.

Li J, “Antitumour and antimicrobial activities of endophytic streptomycetes from pharmaceutical plants in rainforestâ€, Letters in Applied Microbiology, vol. 4. no. 2, pp. 57-62, 2008.

Roy S, Banerjee D, “Isolation Of Antimicrobial Compound By Endophytic Bacteria From Vinca roseaâ€, International Journal Of Current Research, vol. 5, pp. 047-051, 2010.

Cappucino JG, Sherman N, “Microbiology: A Laboratory Manualâ€, California: The Benjamin Cummings Publishing Company, Inc. 1987.

Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST, “Bergey’s Manual of

Determinative Bacteriologyâ€, Ninth Ed. Williams & Wilkins, London, 2005.

Sumpter WC, Miller FM, “The chemistry of heterocyclic compounds, Indole and

carbazole systemsâ€, John Wiley Pub, New York, 2015.

Dhani R, Avinash A, Salenaagina SK, Teja MVS, Masthanainah P, Rathnam R, Silpa

VC, “Indole : The molecule of diverse pharmacological activitiesâ€, Journal of Chemistry Pharmacy, Res.Vol. 3, no. 5, pp. 519-523, 2011.

Taylor WI, Achanzar D, “Catalase test as an aid to the identification of enterobacteriaceae†, Applied Microbiology, vol. 42, no. 1, pp. 58-61, 1972.

Battacharya C, Harsha P, Gupta S, Roy S, “Isolation and characterization of bacterial isolates from agricultural soil at durg districtâ€, Indian Journal Science Research, vol. 4, no. 1, pp. 221-226, 2014.

Shaw N, “Lipid composition as a guide to a classification of bacteriaâ€. Advance Applied Microbiology, vol.17, pp. 63-108, 1975.

Moffitt MC, Neilan BA, “Evolutionary affiliation within the superfamily of ketosynthases reflect complex pathway associationsâ€, Journal of molecular evolution, vol. 56, pp. 446-457, 2002.

Downloads

Published

2017-01-02

How to Cite

Diversity of Endophytic Bacteria Related to Antibacteria Activity Isolated from Vetiveria zizanioides L. (WT). (2017). Asian Journal of Applied Sciences, 4(6). https://ajouronline.com/index.php/AJAS/article/view/4249

Similar Articles

1-10 of 28

You may also start an advanced similarity search for this article.