The Role of <i>L. plantarum</i> as an Immunomodulator Secretion of <i>Transforming Growth Factor</i>-β1, <i>Transforming Growth Factor</i>-β3, and <i>Interferon</i>-α Macrophages and Dermal Fibroblasts


  • Rita Shintawati Biology Department, Indonesia University of Education
  • Sunarjati Sudigdoadi
  • Tita Husnitawati Madjid
  • Endang Sutedja



L.plantarum, TGFb1, TGFb3, Interferon-alfa


This research aims to see the effect of L.plantarum in modulating the secretion of Transforming Growth Factor-TGFβ1, TGFβ3 macrophages and fibroblasts, Interferon-IFNα macrophages, and to analyze the possibility of L.plantarum potency in supporting the process of scarless wound healing. The culture of peritoneal macrophages was treated with L.plantarum for 24 hours, while another macrophage was S.aureus stimulated for 6 hours before treatment of L.plantarum for 24 hours. The formed supernatant was separated and centrifuged to serve as a treatment on the culture of rat dermal fibroblasts for 24 hours. The supernatant was then separated and centrifuged; its cytokine level was measured with enzyme-linked immunosorbent assay-ELISA. Treatment of L.plantarum with medium and high doses increased the secretion of IFNa macrophages compared with the control; all L.plantarum doses can stimulate the secretion of TGFβ1 fibroblast and TGFβ3 macrophage significantly, but it does not affect the secretion of TGFβ1 macrophages. It can be concluded that L.plantarum increased the secretion of IFNα macrophages higher than the treatment preceded by S.aureus stimulation. The secretion of TGFβ1 fibroblasts and TGFβ3 fibroblasts also increased, but it was not as high as L.plantarum treatment stimulated by S.aureus. Therefore, the application of L.plantarum to support the process of wound healing, prophylactic of the excessive scar and fibrosis can be researched further.

Author Biography

Rita Shintawati, Biology Department, Indonesia University of Education

Biology Department


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How to Cite

Shintawati, R., Sudigdoadi, S., Madjid, T. H., & Sutedja, E. (2017). The Role of <i>L. plantarum</i> as an Immunomodulator Secretion of <i>Transforming Growth Factor</i>-β1, <i>Transforming Growth Factor</i>-β3, and <i>Interferon</i>-α Macrophages and Dermal Fibroblasts. Asian Journal of Applied Sciences, 5(2).