Isolation and Identification of <i>Desulfovibrio</i> sp. Bacteria from Acid Sulfate Soil
Keywords:Acid sulfate soils, Desulfovibrio, isolation, pour plate method, SRB
The purpose of this research is to acquire sulfate reducing bacteria (SRB) is used for bioremediation of acid sulfate soils. The method used in this isolation is pour plate method in the medium Desulfovibrio Medium with Lactate under anaeroÂbic conditions. This method consists of dilution stage with physiological saline solution (NaCl 0.9 percent) sterile, inoculation stage in the liquid agar media, testing growth of bacteria isolates on medium Bismuth Sulfite Agar (BSA) with scratches methods, Gram stain test, H2S test and sequencing DNA. Results of the test isolates growth in medium Bismuth Sulfite Agar (BSA) showed bacterial isolates produced have breed well. Gram stain results SRB bacteria isolates showed red cells (Gram-negative) and rod shaped bacillus. H2S test results at Triple Sugar Iron Agar (TSIA) media showed positive results (the formation of H2S). The amplified DNA using the 16S rRNA primers in PCR showed a band of 1324 bp. The analysis of 16S rRNA sequences using BLASTN program shows that bacteria produced has 84% sequence similarity with Desulfovibrio sp..
Altschul, S.F., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J. 1990. Basic local alignment search tool. Mol. Biol. 215: 403-410.
Atlas, R.M. & Parks, L.C. 1993. Handbook of Microbiological Media. Boca Raton: CRC Press.
Badan Karantina Pertanian Departemen Pertanian. 2008. Pedoman Diagnosis Optik Golongan Bakteri. Bogor. pp. 19-32.
Balch, W.E., Fox, G.E., Magrum, L.J., Woese, C.R. & Wolfe, R.S. 1979. Methanogens: reevaluation of a unique biological group. MicroÂ¬biol Rev. 43: 260â€“296.
Benedetto, J.S., de Almeida, S.K., Gomez, H.A., Vazoller, R.F. & Ladeira, A.C.Q. 2005. Monitoring of sulfate-reducing bacteria in acid water from uranium mines. Journal Minerals Engineering 18: 1341-1343.
Das, K.C. & Qin, W. 2012. Isolation and characterization of superior rumen bacteria of cattle (Bos taurus) and potential application in animal feedstuff. Journal of Animal Sciences 2 (4): 224-228.
Djide, M.N. & Sartini. 2008. Analisis Mikrobiologi Farmasi. Cetakan Kedua. Makassar: Fakultas Farmasi Universitas Hasanuddin.
Ekmonsaurus. 2008. Gambar teori pewarnaan bakteri. http://ekmonsaurus.blogspot.com. Diakses pada tanggal 15 Desember 2008.
Fichtel, K., Mathes, F., KÃ¶nneke, M., Cypionka, H. & Engelen, B. 2012. Isolation of sulfate-reducing bacteria from sediments above the deep-subseafloor aquifer. Frontiers in Microbiology Vol.3, Article 65.
Haouari, O., Fardeau, M., Casalot, L., Tholozan, J., Hamdi, M. & Ollivier, B. 2006. Isolation of sulfate-reducing bacteria from Tunisian marine sediments and description of Desulfovibrio bizertensis sp. nov. International Journal of Systematic and Evolutionary Microbiology 56: 2909â€“2913.
Kurokawa, K. 2013. Dasar-dasar mikrobiologi. http://tips-trik-cara-untuk.blogspot.com/2012/04/ dasar-dasar-microbiolog. Diakses pada tanggal 4 Juli 2013.
Pelczar, M.J. & Chan, E.S.C. 1988. Dasar-Dasar Mikrobiologi. Edisi 2. Terjemahan Ratna Siri H, Teja Imas, S. Sutarmi, dan Sri Lestari A. Jakarta: UI-Press.
Sahrani, F.K., Ibrahim, Z., Yahya, A. & Aziz, M. 2008. Isolation and identification of marine sulphate-reducing bacteria, Desulfovibrio sp. and Citrobacter freundii from Pasir Gudang, Malaysia. Sains Malaysiana 37 (4): 365â€“371.
Saida. 2001. Karakterisasi dan ujian aktivitas isolat bakteri pereduksi aulfat asal ekosistem Air Hitam, Kalimantan Tengah. Tesis. Program Pascasarjana Institut Pertanian Bogor.
Wang, L. & Wise, M.J. 2015. Glycogen with short average chain length enhances bacterial durability. http://www.uniprot.org/proteomes/UP000009173. Diakses pada tanggal 9 April 2015.
Warren, Y.A., Citron, D.M., Merriam, C.V. & Goldstein, E.J.C. 2005. Biochemical differentiation and comparison of Desulfovibrio species and other phenotypically similar genera. Journal of Clinical Microbiology 43 (8): 4041â€“4045.
Widdel, F. & Pfennig, N. 1981. Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. I. Isolation of new sulfate-reducing bacteria enriched with acetate from saline environÂ¬ments. Description of Desulfobacter postgatei gen. nov., sp. nov. Arch Microbiol 129: 395â€“400.
Widdel, F. & Bak, F. 1992. Gram-negative mesophilic sulfate-reducing bacteria, in The ProkaryÂ¬otes, eds A. Balows, H.G. TrÃ¼per, M. Dworkin, W. Harder & K.H. Schleifer. New York: SpringerÂ¬Verlag, 3352â€“3378.
Widyati, E. 2006. Bioremediasi tanah bekas tambang batubara dengan sludge industri kertas untuk memacu revegetasi lahan. Disertasi. Sekolah Pascasarjana Institut Pertanian Bogor.
Zamora, A.C. & Malaver, N. 2012. Methodological aspects for the culture and quantification of heterotrophic sulfate-reducing bacteria. Revista de la Sociedad Venezolana de MicrobiologÃa 32: 121-125.
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