Comparison between zeta potential and hyalurenan binding methods to improve sperm selection for intracytoplasmic sperm injection (ICSI)


  • Abd El Wahab El Ghareeb Zoology Department, Faculty of science, Cairo University, Cairo
  • Zainab Alfitouri Department of Zoology, Faculty of Science, Sirt university, Libya
  • Nagla El-Nabarawy National Egyptian Center of Environmental and Toxicological Research (NECTER), Faculty of Medicine, Cairo University, Egypt
  • Amr El Ahwany Department of Andrology, Faculty of Medicine, Cairo University, Egypt
  • Maryam Alzaidy Nile center for IVF, Cairo, Egypt



At present, sperm selection for ICSI only depends on morphology and motility, but these parameters may not be relevant the chromatin integrity. So sperm selection based on sperm functional characterized has been suggested. Thus, aim of this study was the comparison between two sperm selection method, HA binding and Zeta method, to select spermatozoa with normal morphology and intact chromatin.
Methods:Semen samples from 150 infertile couples referring to Nile Center For IVF  were  assessed  during  this  study. Semen  analysis  was carried  out according to WHO criteria. Semen divided in 3 groups. In 2 groups Zeta method and HA binding  applied  to  select  spermatozoa  and  1  group  as  control.  Sperm  morphology, motility and DNA fragmentation were assessed by Papanicolaou staining, Chromomycin A3 (CMA3) staining, and SCD test, respectively.

Both HA binding assay and Zeta method are efficient to select sperm with normal morphology  and  motility.  But  in  term  of  DNA fragmentation Zeta method appear to be more efficient to select sperm with  low DNA fragmentation .
The results of  this study suggest that these sperm  selection method can select spermatozoa with normal morphology and protamine deficiency and can use of selected sperm in ICSI. But Zeta method may be more efficient to select sperm with low DNA fragmentation. In patient with high DNA fragmentation, Zeta method can be useful to select spermatozoa for ICSI.


Hyaluronic acid (HA), Zeta potential, Intra-Cytoplasmic Sperm Injection(ICSI), Morphology, Motility, DNA fragmentation.


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How to Cite

El Ghareeb, A. E. W., Alfitouri, Z., El-Nabarawy, N., El Ahwany, A., & Alzaidy, M. (2017). Comparison between zeta potential and hyalurenan binding methods to improve sperm selection for intracytoplasmic sperm injection (ICSI). Asian Journal of Applied Sciences, 5(4). Retrieved from




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