Comparison between zeta potential and hyalurenan binding methods to improve sperm selection for intracytoplasmic sperm injection (ICSI)
At present, sperm selection for ICSI only depends on morphology and motility, but these parameters may not be relevant the chromatin integrity. So sperm selection based on sperm functional characterized has been suggested. Thus, aim of this study was the comparison between two sperm selection method, HA binding and Zeta method, to select spermatozoa with normal morphology and intact chromatin.
Methods:Semen samples from 150 infertile couples referring to Nile Center For IVFÂ wereÂ assessedÂ duringÂ thisÂ study. SemenÂ analysisÂ was carriedÂ out according to WHO criteria. Semen divided in 3 groups. In 2 groups Zeta method and HA bindingÂ appliedÂ toÂ selectÂ spermatozoaÂ andÂ 1Â groupÂ asÂ control.Â SpermÂ morphology, motility and DNA fragmentation were assessed by Papanicolaou staining, Chromomycin A3 (CMA3) staining, and SCD test, respectively.
Both HA binding assay and Zeta method are efficient to select sperm with normal morphologyÂ andÂ motility.Â ButÂ inÂ termÂ ofÂ DNA fragmentation Zeta method appear to be more efficient to select sperm withÂ low DNA fragmentation .
The results ofÂ this study suggest that these spermÂ selection method can select spermatozoa with normal morphology and protamine deficiency and can use of selected sperm in ICSI. But Zeta method may be more efficient to select sperm with low DNA fragmentation. In patient with high DNA fragmentation, Zeta method can be useful to select spermatozoa for ICSI.
Hyaluronic acid (HA), Zeta potential, Intra-Cytoplasmic Sperm Injection(ICSI), Morphology, Motility, DNA fragmentation.
â€¢ Ai L, Liu SY, Huang J, Chen SW, Liu J & Zhong Y. (2010) Intracyto- plasmic morphologically selected sperm injection of testicular sperm: clinical outcome in azoospermia patients. Zhonghua Nan Ke Xue 16, 826â€“829.
â€¢ Ainsworth C, Nixon B, Aitken RJ (2005) Development of a novel electrophoretic system for the isolation of human spermatozoa. Hum Reprod 20:2261â€“2270.
â€¢ Ainsworth C, Nixon B, Jansen RP & Aitken RJ. (2007) First recorded pregnancy and normal birth after ICSI using electrophoretically iso- lated spermatozoa. Hum Reprod 22, 197â€“200.
â€¢ Antinori M, Licata E, Dani G, Cerusico F, Versaci C, dâ€™Angelo D & Antinori S (2008) Intracytoplasmic morphologically selected sperm injection: a prospective randomized trial. Reprod Biomed Online 16,835â€“841.
â€¢ Arienti G, Carlini E, Palmerini CA (1997) Fusion of human sperm to prostasomes at acidic pH. J Membr Biol 155:89â€“94.
â€¢ AvendanËœ o C, Franchi A, Taylor S, Morshedi M, Bocca S & Oehninger S. (2009) Fragmentation of DNA in morphologically normal human spermatozoa. Fertil Steril 91, 1077â€“1084.
â€¢ Balaban B, Yakin K, Alatas C, Oktem O, Isiklar A & Urman B. (2011) Clinical outcome of intracytoplasmic injection of spermatozoa morphologically selected under high magnification: a prospective randomized study. Reprod Biomed Online 22, 472â€“476.
â€¢ Bartoov B, Berkovitz A, Eltes F (2001) Selection of spermato- zoa with normal nuclei to improve the pregnancy rate with intracytoplasmic sperm injection. N Engl J Med 345:1067â€“1068.
â€¢ Bartoov B, Berkovitz A, Eltes F, Kogosowski A, Menezo Y, Barak Y (2002) Real-time fine morphology of motile human sperm cells is associated with IVF-ICSI outcome. J Androl 23:1â€“8.
â€¢ Bartoov B, Berkovitz A, Eltes F, Kogosovsky A, Yagoda A, Lederman H, Artzi S, Gross M, Barak Y (2003) Pregnancy rates are higher with intracytoplasmic morphologically selected sperm injection than with conventional intracyto- plasmic injection. Fertil Steril 80:1413â€“1419.
â€¢ Bastiaan HS, Windt ML, Menkveld R, Kruger TF, Oehninger S & Fran- ken DR. (2003) Relationship between zona pellucida-induced acro- some reaction, sperm morphology, sperm-zona pellucida binding, and in vitro fertilization. Fertil Steril 79, 49â€“55.
â€¢ Berkovitz A, Eltes F, Yaari S, Katz N, Barr I, Fishman A, Bartoov B (2005) The morphological normalcy of the sperm nucleus and pregnancy rate of intracytoplasmic injection with morphologically selected sperm. Hum Reprod 20:185â€“190.
â€¢ Berkovitz A, Eltes F, Lederman H, Peer S, Ellenbogen A, Feldberg B, Bartoov B (2006) How to improve IVF-ICSI out- come by sperm selection. Reprod Biomed Online 12:634â€“638.
â€¢ Black M, Liu de Y, Bourne H & Baker HW. (2010) Comparison of outcomes of conventional intracytoplasmic sperm injection and intracytoplasmic sperm injection using sperm bound to the zonapel- lucida of immature oocytes. Fertil Steril 93, 672â€“674.
â€¢ Casper RF, Meriano JS, Jarvi KA, Cowan L & Lucato ML. (1996) The hypo-osmotic swelling test for selection of viable sperm for intracy- toplasmic sperm injection in men with complete asthenozoospermia. Fertil Steril 65, 972â€“976.
â€¢ Cayli S, Jakab A, Ovari L (2003) Biochemical markers of sperm function: male fertility and sperm selection for ICSI. Reprod Biomed Online 7:462â€“468.
â€¢ Cayli S, Sakkas D, Vigue L, Demir R, Huszar G (2004) Cellular maturity and apoptosis in human sperm: creatine kinase, caspase-3 and Bcl-XL levels in mature and diminished maturity sperm. Mol Hum Reprod 10:365â€“372.
â€¢ Celik-Ozenci C, Catalanotti J, Jakab A, Aksu C, Ward D, Bray-Ward P, Demir R, Huszar G (2003) Human sperm maintain their shape following decondensation and denatur- ation for fluorescent in situ hybridization: shape analysis and objective morphometry. Biol Reprod 69:1347â€“1355.
â€¢ Celik-Ozenci C, Jakab A, Kovacs T, Catalanotti J, Demir R, Bray-Ward P, Ward D, Huszar G (2004) Sperm selection for ICSI: shape properties do not predict the absence or presence of numerical chromosomal aberrations. Hum Reprod 19:2052â€“2059.
â€¢ Chan PJ, Jacobson JD, Corselli JU, Patton WC (2006) A sim- ple zeta method for sperm selection based on membrane charge. Fertil Steril 85:481â€“486.
â€¢ Check JH, Katsoff D, Check ML, Choe JK & Swenson K. (2001) In vitro fertilization with intracytoplasmic sperm injection is an effective therapy for male factor infertility related to subnormal hypo-osmotic swelling test scores. J Androl 22, 261â€“265.
â€¢ Cherr GN, Yudin AI, Li MW (1999) Hyaluronic acid and the cumulus extracellular matrix induce increases in intracellular calcium in macaque sperm via the plasma membrane protein PH-20. Zygote 7:211â€“222.
â€¢ Cherr GN, Yudin AI & Overstreet JW. (2001) The dual functions of GPI-anchored PH-20: hyaluronidase and intracellular signaling. Matrix Biol 20, 515â€“525.
â€¢ De VanteÂ´ ryArrighi C, Lucas H, Chardonnens D & de Agostini A. (2009) Removal of spermatozoa with externalized phosphatidylser- ine from sperm preparation in human assisted medical procreation: effects on viability, motility and mitochondrial membrane potential. Reprod Biol Endocrinol 7, 1.
â€¢ De Vos A, Van De Velde H, Joris H, Verheyen G, Devroey P & Van Steirteghem A. (2003) Influence of individual sperm morphology on fertilization, embryo morphology, and pregnancy outcome of in- tracytoplasmic sperm injection. Fertil Steril 79, 42â€“48.
â€¢ Deemeh MR, Tavalaee M, Ahmadi SM, Kalantari SA, Alavi Nasab SV, Najafi MH & Nasr Esfahani MH. (2010) The first report of successfully pregnancy after ICSI with combined DGC â„ Zeta sperm selection procedure in a couple with eleven repeated fail IVF â„ ICSI cycles. IJFS 4, 41â€“43.
â€¢ Della Giovampaola C, Flori F, Sabatini L, Incerti L, La Sala GB, Rosati F, Focarelli R (2001) Surface of human sperm bears three differently charged CD52 forms, two of which remain tably bound to sperm after capacitation. Mol Reprod Dev 60:89â€“96.
â€¢ Dirican EK, OzguÂ¨ n OD, Akarsu S, Akin KO, Ercan O, UgË˜ urlu M, Camsari C, Kanyilmaz O, Kaya A & Unsal A. (2008) Clinical out- come of magnetic activated cell sorting of non-apoptotic spermato- zoa before density gradient centrifugation for assisted reproduction. J Assist Reprod Genet 25, 375â€“381.
â€¢ Drobnis EZ, Zhong CQ & Overstreet JW. (1991) Separation of cryop- reserved human semen using Sephadex columns, washing, or Percoll gradients. J Androl 12, 201â€“208.
â€¢ Edwards RG, Bavister BD & Steptoe PC. (1969) Early stages of fertil- ization in vitro of human oocytes matured in vitro. Nature 221, 632â€“635.
â€¢ Engelmann U, Krassnigg F, Schatz H, Schill WB (1988) Separation of human X and Y spermatozoa by free-flow electrophoresis. Gamete Res 19:151â€“160.
â€¢ Ermini L, Secciani F, La Sala GB, Sabatini L, Fineschi D, Hale G, Rosati F (2005) Different glycoforms of the human GPI-anchored antigen CD52 associate differently with lipid microdomains in leukocytes and sperm membranes. Biochem Biophys Res Commun 338:1275â€“1283.
â€¢ Fleming SD, Ilad RS, Griffin AM, Wu Y, Ong KJ, Smith HC & Ait- ken RJ. (2008) Prospective controlled trial of an electrophoretic method of sperm preparation for assisted reproduction: compari- son with density gradient centrifugation. Hum Reprod 23, 2646â€“ 2651.
â€¢ Focarelli R, Rosati F, Terrana B (1990) Sialyglycoconjugates release during in vitro capacitation of human spermatozoa. J Androl 11:97â€“104.
â€¢ Franken DR & Bastiaan HS. (2009) Can a cumulus cell complex be used to select spermatozoa for assisted reproduction? Andrologia 41, 369â€“376.
â€¢ Franken DR & Oehninger S. (2006) The clinical significance of sperm- zona pellucida binding: 17 years later. Front Biosci 11, 1227â€“1233.
â€¢ Gergely A, Kovanci E, Senturk L (1999) Morphometrical assessment of mature and diminished maturity human spermatozoa: sperm regions that reflect differences in matu- rity. Hum Reprod 14:2007â€“2014.
â€¢ Gianaroli L, Magli MC, Cavallini G, Crippa A, Nadalini M, Bernardini L, MenchiniFabris GF, Voliani S & Ferraretti AP. (2005) Frequency of aneuploidy in sperm from patients with extremely severe male factor infertility. Hum Reprod 20, 2140â€“2152.
â€¢ Gianaroli L, Magli MC, Collodel G, Moretti E, Ferraretti AP & Baccetti B. (2008) Sperm headâ€™s birefringence: a new criterion for sperm selection. Fertil Steril 90, 104â€“112.
â€¢ Gianaroli L, Magli MC, Ferraretti AP, Crippa A, Lappi M, Capitani S
â€¢ & Baccetti B. (2010) Birefringence characteristics in sperm heads allow for the selection of reacted spermatozoa for intracytoplasmic sperm injection. Fertil Steril 93, 807â€“813.
â€¢ Glander HJ & Schaller J. (1999) Binding of annexin V to plasma membranes of human spermatozoa: a rapid assay for detection of membrane changes after cryostorage. Mol Hum Reprod 5, 109â€“115.
â€¢ Grunewald S, Paasch U, Glander HJ (2001) Enrichment of non-apoptotic human spermatozoa after cryopreservation by immunomagnetic cell sorting. Cell Tissue Bank 2:127â€“133.
â€¢ Hazout A, Dumont-Hassan M, Junca AM, Cohen Bacrie P & Tesarik J. (2006) High-magnification ICSI overcomes paternal effect resistant to conventional ICSI. Reprod Biomed Online 12, 19â€“25.
â€¢ Henkel RR, Schill WB (2003) Sperm preparation for ART. Reprod Biol Endocrinol 14:108.
â€¢ Hong SJ, Chiu PC, Lee KF, Tse JM, Ho PC & Yeung WS. (2004) Establishment of a capillary-cumulus model to study the selection of sperm for fertilization by the cumulus oophorus. Hum Reprod 19, 1562â€“1569.
â€¢ Huszar G, Ozenci CC, Cayli S, Zavaczki Z, Hansch E & Vigue L. (2003) Hyaluronic acid binding by human sperm indicates cellular maturity, viability, and unreacted acrosomal status. Fertil Steril 79, 1616â€“1624.
â€¢ Huszar G, Sbracia M, Vigue L, Miller DJ, Shur BD (1997) Sperm plasma membrane remodeling during spermiogenetic maturation in men: relationship among plasma membrane beta1,4-galactosyltransferase, cytoplasmic creatine phospho- kinase, and creatine hosphokinase isoform ratios. Biol Reprod 56:1020â€“1024.
â€¢ Huszar G, Celik-Ozenci C, Cayli S, Kovacs T, Vigue L, Kovanci E (2004) Semen characteristics after overnight shipping: preservation of sperm concentrations, HspA2 ratios, CK activity, cytoplasmic retention, chromatin matu- rity, DNA integrity, and sperm shape. J Androl 25:593â€“604.
â€¢ Huszar G, Jakab A, Sakkas D, Ozenci CC, Cayli S, Delpiano E, Ozkavukcu S (2007) Fertility testing and ICSI sperm selection by hyaluronic acid binding: clinical and genetic aspects. Reprod Biomed Online 14:650â€“663.
â€¢ Ishijima SA, Okuno M, Mohri H (1991) Zeta potential of human X- and Y-bearing sperm. Int J Androl 14:340â€“347.
â€¢ Jakab A, Sakkas D, Delpiano E, Cayli S, Kovanci E, Ward D, Revelli A, Huszar G (2005) Intracytoplasmic sperm injection: a novel selection method for sperm with normal frequency of chromosomal aneuploidies. Fertil Steril 84:1665â€“1673.
â€¢ Janssens R, Verheyen G & Bocken G. (2006) Use of PICSI Dishes for Sperm Selection in Clinical ICSI Practice: Results of a Pilot Study. Abstracts of Annual Meeting of the Belgian Society Reproductive Medicine.
â€¢ Kaneko S, Oshio S, Kobayashi T, Iizuka R, Mohri H (1984) Human X- and Y-bearing sperm differ in cell surface sialic acid content. Biochem Biophys Res Commun 124:950â€“955.
â€¢ Kang JH & Park J. (2005) Cell separation technology. APBMT 9, 1139â€“2005.
â€¢ Khajavi NA, Razavi Sh, Mardani M, Tavalaee M, Deemeh MR & Nasr- Esfahani MH. (2009) Can Zeta sperm selection method, recover sperm with higher DNA integrity compare to density gradient centrifugation? IJRM 7, 73â€“77.
â€¢ Kheirollahi-Kouhestani M, Razavi S, Tavalaee M, Deemeh MR, Marda- ni M, Moshtaghian J & Nasr-Esfahani MH. (2009) Selection of sperm based on combined density gradient and Zeta method may improve ICSI outcome. Hum Reprod 24, 2409â€“2416.
â€¢ Kirchhoff C, Hale G (1996) Cell-to-cell transfer of glycosyl- phosphatidylinositol-anchored membrane proteins during sperm maturation. Mol Hum Reprod 2:177â€“184.
â€¢ Kordus RJ, Price RL, Davis JM & Whitman-Elia GF. (2008) Success- ful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: a case report. J Assist Reprod Genet 25, 437â€“443.
â€¢ Kruger TF, Menkveld R, Stander FS, Lombard CJ, Van der Merwe JP, van Zyl JA, Smith K (1986) Sperm morphologic features as a prognostic factor in in vitro fertilization. Fertil Steril 46:1118â€“1123.
â€¢ Kruger TF, DuToit TC, Franken DR, Acosta AA, Oehninger SC, Menkveld R, Lombard CJ (1993) A new computerized method of reading sperm morphology (strict criteria) is as efficient as technician reading. Fertil Steril 59:202â€“209.
â€¢ Liu DY, Garrett C & Baker HW. (2003) Low proportions of sperm can bind to the zona pellucida of human oocytes. Hum Reprod 18, 2382â€“2389.
â€¢ Liu F, Qiu Y, Zou Y, Deng ZH, Yang H & Liu de Y. (2011) Use of zona pellucida-bound sperm for intracytoplasmic sperm injection produces higher embryo quality and implantation than conventional intracytoplasmic sperm injection. Fertil Steril 95, 815â€“818.
â€¢ Lopata A, Patullo MJ, Chang A, James B (1976) A method for collecting motile spermatozoa from human semen. Fertil Steril 27:677â€“684.
â€¢ Lucas H, Lammers J, Pfeffer J, Aknin I, CarreÂ´ -Pigeon F, Jafou N, Pau- lus JM & Sifer C. (2010) Conventional IVF versus ICSI in sibling oocytes: A French experience analysis for BLEFCO. Gynecol Obstet Fertil 38, 515â€“520.
â€¢ Mahadevan M & Baker G. (1984) Assessment and preparation of semenfor in vitro fertilization. In: Clinical In Vitro Fertilization (eds C Wood & A Trounson), pp. 83â€“97. Springer-Verlag, Berlin, Germany.
â€¢ Mauri AL, Petersen CG, Oliveira JB, Massaro FC, Baruffi RL & Franco JG Jr. (2010) Comparison of day 2 embryo quality after conven- tional ICSI versus intracytoplasmic morphologically selected sperm injection (IMSI) using sibling oocytes. Eur J Obstet Gynecol Reprod Biol 150, 42â€“46.
â€¢ Morrell JM, Moffatt O, Sakkas D, Manicardi GC, Bizzaro D, Tomlinson M, Nilsson H, Holmes PV (2004) Reduced senescence and retained nuclear DNA integrity in human spermatozoa prepared by density gradient centrifugation. J Assist Reprod Genet 21:217â€“222.
â€¢ Nadalini M, Tarozzi N, Distratis V, Scaravelli G & Borini A. (2009) Impact of intracytoplasmic morphologically selected sperm injection on assisted reproduction outcome: a review. Reproductive Bio Medi- cine Online 3, 45â€“55.
â€¢ Nagy ZP, Liu J, Joris H, Verheyen G, Tournaye H, Camus M, Derde MC, Devroey P & Van Steirteghem AC. (1995) The result of in- tracytoplasmic sperm injection is not related to any of the three basic sperm parameters. Hum Reprod 10, 1123â€“1129.
â€¢ Nasr-Esfahani MH, Razavi S, Mardani M (2001) Relation between different human sperm nuclear maturity tests and in vitro fertilization. J Assist Reprod Genet 18:219â€“225.
â€¢ Nasr-Esfahani MH, Razavi S, Vahdati D, Fathi F, Tavalaee M (2008a) Evaluation of sperm selection procedure based on hyaluronic acid binding ability on ICSI outcome. J Assist Reprod Genet 25:197â€“203.
â€¢ Nasr-Esfahani MH, Razavi SH, Tavalaee M (2008b) Failed fertilization post ICSI and spermiogenic defects. Fertil Steril 89:892â€“898.
â€¢ Oehninger S. (2003) Biochemical and functional characterization of the human zona pellucida. Reproductive Bio Medicine Online 7, 641â€“648.
â€¢ Paes Almeida Ferreira de Braga D, Iaconelli A Jr, CaÂ´ ssia SaÂ´ vio de Figueira R, Madaschi C, SemiaËœo-Francisco L & Borges E Jr. (2009) Outcome of ICSI using zona pellucida-bound spermatozoa and conventionally selected spermatozoa. Reproductive Bio Medicine Online 19, 802â€“807.
â€¢ Palermo G, Joris H, Devroey P, Van Steirteghem AC (1992) Pregnancies after intracytoplasmic injection of single sper- matozoon into an oocyte. Lancet 340:17â€“18.
â€¢ Parmegiani L, Cognigni GE, Bernardi S, Troilo E, Ciampaglia W & Filicori M. (2010a) â€˜Physiologic ICSIâ€™: hyaluronic acid (HA) favors selection of spermatozoa without DNA fragmentation and with normal nucleus, resulting in improvement of embryo quality. Fertil Steril 93, 598â€“604.
â€¢ Parmegiani L, Cognigni GE, Ciampaglia W, Pocognoli P, Marchi F & Filicori M. (2010b) Efficiency of hyaluronic acid (HA) sperm selection. J Assist Reprod Genet 27, 13â€“16.
â€¢ Peer S, Eltes F, Berkovitz A, Yehuda R, Itsykson P & Bartoov B. (2007) Is fine morphology of the human sperm nuclei affected by in vitro incubation at 37 degrees C? Fertil Steril 88, 1589â€“1594.
â€¢ Petersen CG, Vagnini LD, Mauri AL, Massaro FC, Cavagna M, Baruffi RL, Oliveira JB & Franco JG Jr. (2011) Relationship between DNA damage and sperm head birefringence. Reprod Biomed Online 22, 583â€“589.
â€¢ Polak de Fried E & Denaday F. (2010) Single and twin ongoing preg- nancies in two cases of previous ART failure after ICSI performed with sperm sorted using annexin V microbeads. Fertil Steril 94, 351.
â€¢ Pousette A, AkerloÂ¨ f E, Rosenborg L & Fredricsson B. (1986) Increase in progressive motility and improved morphology of human spermatozoa following their migration through Percoll gradients. Int J Androl 9, 1â€“13.
â€¢ Prinosilova P, Kruger T, Sati L, Ozkavukcu S, Vigue L, Kovanci E & Huszar G. (2009) Selectivity of hyaluronic acid binding for sperma- tozoa with normal Tygerberg strict morphology. Reprod Biomed Online 18, 177â€“183.
â€¢ Rawe VY, Boudri HU, Alvarez SedoÂ´ C, Carro M, Papier S & Nodar F. (2010) Healthy baby born after reduction of sperm DNA fragmenta- tion using cell sorting before ICSI. Reprod Biomed Online 20, 320â€“323.
â€¢ Razavi S, Nasr-Esfahani MH, Mardani M, Mafi A, Moghdam A (2003) Effect of human sperm chromatin anomalies on fertilization outcome post-ICSI. Andrologia 35:238â€“243.
â€¢ Razavi SH, Nasr-Esfahani MH, Deemeh MR, Shayesteh M & Tavalaee M. (2010) Evaluation of zeta and HA-binding methods for selection of spermatozoa with normal morphology, protamine content and DNA integrity. Andrologia 42, 13â€“19.
â€¢ Rijsdijk M & Franken DR. (2007) Use of the capillary-cumulus oopho- rus model for evaluating the selection of spermatozoa. Fertil Steril 88, 1595â€“1602.
â€¢ Rooney IA, Heuser JE, Atkinson JP (1996) GPI-anchored complement regulatory proteins in seminal plasma. An analysis of their physical condition and the mechanisms of their binding to exogenous cells. J Clin Invest 97:1675â€“1686.
â€¢ Said T, Agarwal A, Grunewald S, Rasch M, Glander HJ & Paasch U. (2006a) Evaluation of sperm recovery following annexin V mag- netic-activated cell sorting separation. Reprod Biomed Online 13, 336â€“339.
â€¢ Said T, Agarwal A, Grunewald S, Rasch M, Baumann T, Kriegel C, Li L, Glander HJ, Thomas AJ Jr & Paasch U. (2006b) Selection of non apoptotic spermatozoa as a new tool for enhancing assisted reproduction outcomes: an in vitro model. Biol Reprod 74, 530â€“537.
â€¢ Salustri A, Camaioni A, Di Giacomo M, Fulop C & Hascall VC. (1999) Hyaluronan and proteoglycans in ovarian follicles. Hum Reprod Update 5, 293â€“301.
â€¢ Sanchez M, Aran B & Blanco J. (2005) Preliminary Clinical and FISH Results on Hyaluronic Acid Sperm Selection to Improve ICSI. Abstracts of the 21st Annual Meeting of the ESHRE. Oxford Univer- sity Press, Oxford, UK.
â€¢ Schmitz B, Radbruch A, KuÂ¨ mmel T, Wickenhauser C, Korb H, Hans- mann ML, Thiele J & Fischer R. (1994) Magnetic activated cell sort- ing (MACS) â€“ a new immunomagnetic method for megakaryocytic cell isolation: comparison of different separation techniques. Eur J Haematol 52, 267â€“275.
â€¢ SchroÂ¨ ter S, Kirchhoff C, Yeung CH & Cooper T. (1997) Meyer B. Purification and structural analysis of sperm CD52, a GPI-anchored membrane protein. Adv Exp Med Biol 424, 233â€“234.
â€¢ Seli E, Sakkas D (2005) Spermatozoal nuclear determinants of reproductive outcome: implications for ART. Hum Reprod Update 11:337â€“349.
â€¢ Shoukir Y, Chardonnens D, Campana A & Sakkas D. (1998) Blastocyst development from supernumerary embryos after intracytoplasmic sperm injection: a paternal influence? Hum Reprod Update 13, 1632â€“1637.
â€¢ Soleimani M, Tavalaee M, Aboutorabi R, Adib M, Bahramian H, Janz- amin E, Kiani A & Nasr-Esfahani MH. (2010) Evaluation of Fas positive sperm and complement mediated lysis in subfertile individ- uals. J Assist Reprod Genet 27, 477â€“482.
â€¢ Souza Setti A, Ferreira RC, Paes de Almeida Ferreira Braga D, de CaÂ´ s- sia SaÂ´ vio Figueira R, Iaconelli A Jr & Borges E Jr. (2010) Intracyto- plasmic sperm injection outcome versus intracytoplasmic morphologically selected sperm injection outcome: a meta-analysis. Reprod Biomed Online 21, 450â€“455.
â€¢ Srivastava PN, Farooqui AA (1980) Studies on neuraminidase activity of the rabbit endometrium. Biol Reprod 22:858â€“863.
â€¢ Stanger JD, Vo L, Yovich JL & Almahbobi G. (2010) Hypo-osmotic swelling test identifies individual spermatozoa with minimal DNA fragmentation. Reprod Biomed Online 21, 474â€“484.
â€¢ Suarez SS & Pacey AA. (2006) Sperm transport in the female repro- ductive tract. Hum Reprod Update 12, 23â€“37.
â€¢ Sutovsky P. (2003) Ubiquitin-dependent proteolysis in mammalian spermatogenesis, fertilization, and sperm quality control: killing three birds with one stone. Microsc Res Tech 61, 88â€“102. Review
â€¢ Tavalaee M, Razavi R, Nasr-Esfahani MH (2009) Influence of sperm chromatin anomalies on assisted reproductive technology outcome. Fertil Steril 91:1119â€“1126. in press.
â€¢ Van der Ven HH, Jeyendran RS, Al-Hasani S, TuÂ¨ nnerhoff A, Hoebbel K, Diedrich K, Krebs D & Perez-Pelaez M. (1988) Glass wool col- umn filtration of human semen: relation to swim-up procedure and outcome of IVF. Hum Reprod Update 3, 85â€“88.
â€¢ Ved S, Montag M, Schmutzler A, Prietl G, Haidl G & van der Ven H. (1997) Pregnancy following intracytoplasmic sperm injection of immotile spermatozoa selected by the hypo-osmotic swelling-test: a case report. Andrologia 29, 241â€“242.
â€¢ Vines CA, Li MW, Deng X (2001) Identification of a hyaluronic acid (HA) binding domain in the PH-20 protein that may function in cell signaling. Mol Reprod Dev 60:542â€“552.
â€¢ Wilding M, Coppola G, di Matteo L, Palagiano A, Fusco E & Dale B. (2011) Intracytoplasmic injection of morphologically selected sper- matozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa. J Assist Reprod Genet 28, 253â€“262.
â€¢ World Health Organization (1999) WHO Laboratory Manual for the Examination of Human Semen and Spermâ€“Cervical Mucus Interaction. Cambridge University Press, Cambridge, UK.
â€¢ Worrilow KC, Eid S, Matthews JM, Pelts EJ, Khoury C & Liebermann J. (2009) A multi-site clinical trial evaluating PICSI versus intracy- toplasmic sperm injection (ICSI): positive clinical outcomes observed in a prospective, randomized and double-blinded study. Fertil Steril 92, S36â€“S37.
â€¢ Yagci A, Murk W, Stronk J & Huszar G. (2010) Spermatozoa bound to solid state hyaluronic acid show chromatin structure with high NA chain integrity: an acridine orange fluorescence study. J Androl 31, 566â€“572.
â€¢ Yeung CH, Cooper TG, Nieschlag E (1997) Human epididy- mal secreted protein CD52 on ejaculated spermatozoa: correlations with semen characteristics and the effect of its antibody. Mol Hum Reprod 3:1045â€“1051.
â€¢ Yudin AI, Vandevoort CA, Li MW & Overstreet JW. (1999) PH-20 but not acrosin is involved in sperm penetration of the macaque zona pellucida. Mol Reprod Dev 53, 350â€“362.
â€¢ Zhang K, Zhu W, Fan L & Gong F. (2010) Human normal sperm morphology rate and in vitro fertilization outcome. Zhong Nan Da Xue Xue Bao Yi Xue Ban 35, 738â€“742.
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