Anti-microbial Activities of <em>Pseudomonas</em> Metabolites Methanolic Extracts against Different MDR Bacterial Pathogens

Authors

  • Ubaid Rasheed Department of Microbiology, Kohat University of Science and Technology , Pakistan
  • Faiza Momin Department of Microbiology, Kohat University of Science and Technology , Pakistan
  • Hikmat Ullah Department of Microbiology, Kohat University of Science and Technology, Pakistan
  • Imad Ali Department of Microbiology, Kohat University of Science and Technology, Pakistan
  • Haq Nawaz Department of Microbiology, Kohat University of Science and Technology , Pakistan
  • Latif Ahmad Department of Microbiology, Kohat University of Science and Technology, Pakistan
  • Abid Khan Center of Biotechnology & Microbiology (COBAM), University of Peshawar, Pakistan
  • Mahideen Afridi National Center for Bioinformatics, Quaid-i-Azam University, Islamabad, Pakistan

DOI:

https://doi.org/10.24203/ajas.v9i5.6718

Keywords:

MDR, Pseudomonas, Streptomyces, Bacillus and TLC

Abstract

Background: A Multiple drug resistance (MDR) in pathogenic bacteria has become a significant public health issue for treatment. Among bacteria, Pseudomonas is another important genus except Streptomyces and Bacillus in production of antimicrobial compounds. The current study aim to to determine the antibacterial activity and preliminary characterization of antibacterial compounds produced by Pseudomonas species such as BB1D11, BN2D41, TG1D11, TR1D41, LH1D11 and TN1D41.

Method: The antibacterial activity was checked by using bio autography method as well as agar well diffusion method, against four multiple drug resistant bacteria including three Gram negative bacteria (E.coli, Acinetobacter and Pseudomonas) and one Gram positive bacterium (Methicillin Resistant Staphylococcus aureus). Isolation test showed good activity against all the four MDR bacteria, by producing clear zone of diameter from 2mm up to 20mm. Optimum temperature for growth and antibiotic production of Pseudomonas BB1D11, BN2D41, TG1D11, TR1D41, LH1D11 and TN1D41 was 37C0. It produces more metabolites when subjected to shaking incubation. In thin layer chromatography, the extracts were repeatedly inserted on a silica gel coated plate, which was run in mobile phase. Normal HPLC was perform to reveal the presence of antibacterial compounds.

Results:  By well diffusion assay a zone of inhibition range from 2-18 mm of diameter against different test bacteria. The components were separated, resulting in the formation of bands with different colors, each showing a different compounds. Biological screening was performed by bio autography, metabolites showed a significant activity at retention factor of 0.89. While HPLC at retention time 2.50-2.90 showed presence of significant antibacterial compounds.

Conclusion:  Pseudomonas BB1D11, BN2D41, TG1D11, TR1D41, LH1D11 and TN1D41 showed promising anti-microbial activity against different MDR bacteria. It is concluded that HPLC revealed the presence of DAPG at retention time 2.90 which inhibit the growth of MDR bacterial strains.

References

Infectious Diseases Society of America (IDSA). (2011). Combating antimicrobial resistance: policy recommendations to save lives. Clinical Infectious Diseases, 52(suppl_5), S397-S428.

Vardakas, K. Z., Rafailidis, P. I., Konstantelias, A. A., & Falagas, M. E. (2013). Predictors of mortality in patients with infections due to multi-drug resistant Gram negative bacteria: the study, the patient, the bug or the drug?. Journal of Infection, 66(5), 401-414.

Bodi, M., Ardanuy, C., & Rello, J. (2001). Impact of Gram-positive resistance on outcome of nosocomial pneumonia. Critical care medicine, 29(4), N82-N86.

Perez, F., & Van Duin, D. (2013). Carbapenem-resistant Enterobacteriaceae: a menace to our most vulnerable patients. Cleveland Clinic journal of medicine, 80(4), 225.

Ena, J., Dick, R. W., Jones, R. N., & Wenzel, R. P. (1993). The epidemiology of intravenous vancomycin usage in a university hospital: a 10-year study. Jama, 269(5), 598-602.

Costerton, J. W., Stewart, P. S., & Greenberg, E. P. (1999). Bacterial biofilms: a common cause of persistent infections. Science, 284(5418), 1318-1322.

Davies, J., & Davies, D. (2010). Origins and evolution of antibiotic resistance. Microbiology and molecular biology reviews, 74(3), 417-433.

Giedraitienė, A., Vitkauskienė, A., Naginienė, R., & Pavilonis, A. (2011). Antibiotic resistance mechanisms of clinically important bacteria. Medicina, 47 (3), 19.

Reidy, B., Haase, A., Luch, A., Dawson, K. A., & Lynch, I. (2013). Mechanisms of silver nanoparticle release, transformation and toxicity: a critical review of current knowledge and recommendations for future studies and applications. Materials, 6(6), 2295-2350.

Codex, P. (1979). Incorporating the British Pharmaceutical Codex.

Nascimento, G. G., Locatelli, J., Freitas, P. C., & Silva, G. L. (2000). Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian journal of microbiology, 31, 247-256.

Grundmann, H., Aires-de-Sousa, M., Boyce, J., & Tiemersma, E. (2006). Emergence and resurgence of meticillin-resistant Staphylococcus aureus as a public-health threat. The lancet, 368(9538), 874-885.

David, M. Z., & Daum, R. S. (2010). Community-associated methicillin-resistant Staphylococcus aureus: epidemiology and clinical consequences of an emerging epidemic. Clinical microbiology reviews, 23(3), 616-687.

Drlica, K. (2001). A strategy for fighting antibiotic resistance. ASM news, 67, 27-33.

Nikaido, H. (2009). Multidrug resistance in bacteria. Annual review of biochemistry, 78, 119-146.

Ventola, C. L. (2015). The antibiotic resistance crisis: part 1: causes and threats. Pharmacy and therapeutics, 40(4), 277.

Allen, H. K., Donato, J., Wang, H. H., Cloud-Hansen, K. A., Davies, J., & Handelsman, J. (2010). Call of the wild: antibiotic resistance genes in natural environments. Nature Reviews Microbiology, 8(4), 251-259..

Ventola, C. L. (2015). The antibiotic resistance crisis: part 1: causes and threats. Pharmacy and therapeutics, 40(4), 277

Jacobsen, F., Fisahn, C., Sorkin, M., Thiele, I., Hirsch, T., Stricker, I., ... & Steinstraesser, L. (2011). Efficacy of topically delivered moxifloxacin against wound infection by Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus. Antimicrobial agents and chemotherapy, 55(5), 2325-2334.

Yilmaz, M., Soran, H., & Beyatli, Y. (2006). Antimicrobial activities of some Bacillus spp. strains isolated from the soil. Microbiological research, 161(2), 127-131.

Gebreel, H. M., El-Mehalawy, A. A., El-Kholy, I. M., Rifaat, H. M., & Humid, A. A. (2008). Antimicrobial activities of certain bacteria isolated from Egyptian soil against pathogenic fungi. Research Journal of Agriculture and Biological Sciences, 4(4), 331-339.

Hilty, D. M., Ferrer, D. C., Parish, M. B., Johnston, B., Callahan, E. J., & Yellowlees, P. M. (2013). The effectiveness of telemental health: a 2013 review. Telemedicine and e-Health, 19(6), 444-454.

Sun, J., Deng, Z., & Yan, A. (2014). Bacterial multidrug efflux pumps: mechanisms, physiology and pharmacological exploitations. Biochemical and biophysical research communications, 453(2), 254-267.

Abbot, P., Abe, J., Alcock, J., Alizon, S., Alpedrinha, J. A., Andersson, M., ... & Zink, A. (2011). Inclusive fitness theory and eusociality. Nature, 471(7339), E1-E4.

Hu, J., Chen, G., & Lo, I. M. (2005). Removal and recovery of Cr (VI) from wastewater by maghemite nanoparticles. Water research, 39(18), 4528-4536.

Ayyadurai, N., Ravindra Naik, P., Sreehari Rao, M., Sunish Kumar, R., Samrat, S. K., Manohar, M., & Sakthivel, N. (2006). Isolation and characterization of a novel banana rhizosphere bacterium as fungal antagonist and microbial adjuvant in micropropagation of banana. Journal of Applied Microbiology, 100(5), 926-937.

Al-Dhabi, N. A., Esmail, G. A., Duraipandiyan, V., Arasu, M. V., & Salem-Bekhit, M. M. (2016). Isolation, identification and screening of antimicrobial thermophilic Streptomyces sp. Al-Dhabi-1 isolated from Tharban hot spring, Saudi Arabia. Extremophiles, 20(1), 79-90.

Mezaache-Aichour, S., Guechi, A., Zerroug, M. M., Nicklin, J., & Strange, R. N. (2013). Antimicrobial activity of Pseudomonas secondary metabolites. Pharmacognosy Communications, 3(3).

Sunkar, S., & Nachiyar, C. V. (2013). Endophytic fungi mediated extracellular silver nanoparticles as effective antibacterial agents. Int J Pharm Pharm Sci, 5(2), 95-100.

Isnansetyo, A., & Kamei, Y. (2003). MC21-A, a bactericidal antibiotic produced by a new marine bacterium, Pseudoalteromonas phenolica sp. nov. O-BC30T, against methicillin-resistant Staphylococcus aureus. Antimicrobial agents and chemotherapy, 47(2), 480-488.

Commare, R. R., Nandakumar, R., Kandan, A., Suresh, S., Bharathi, M., Raguchander, T., & Samiyappan, R. (2002). Pseudomonas fluorescens based bio-formulation for the management of sheath blight disease and leaffolder insect in rice. Crop Protection, 21(8), 671-677.

Garcia-Rosales, C., Eckstein, W., & Roth, J. (1995). Revised formulae for sputtering data. Journal of Nuclear Materials, 218(1), 8-17.

Zhou, M., Li, L., Dunson, D., & Carin, L. (2012). Lognormal and gamma mixed negative binomial regression. In Proceedings of the... International Conference on Machine Learning. International Conference on Machine Learning (Vol. 2012, p. 1343). NIH Public Access.

Saravanan, T., & Muthusamy, M. (2006). Influence of Fusarium oxysporum f. sp. cubense (ef smith) Snyder and Hansen on 2, 4-diacetylphloroglucinol production by Pseudomonas fluorescens migula in banana rhizosphere. Journal of plant protection research, 241-253

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Published

2021-11-04

How to Cite

Rasheed, U., Momin, F., Ullah, H. ., Ali, I., Nawaz, H., Ahmad, L., Khan, A. ., & Afridi, M. . (2021). Anti-microbial Activities of <em>Pseudomonas</em> Metabolites Methanolic Extracts against Different MDR Bacterial Pathogens. Asian Journal of Applied Sciences, 9(5). https://doi.org/10.24203/ajas.v9i5.6718

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