Coconut Milk, Soy Milk, and Fish Flour as <i>Bacillus sphaericus’s </i>Alternative Media Material Used in the Control of <i>Anopheles sp.</i>


  • Zainal Fikri Polytechnic of Health Ministry of Health Mataram
  • Yunan Jiwintarum Polytechnic of Health Ministry of Health Mataram
  • Maruni Wiwin Diarti Polytechnic of Health Ministry of Health Mataram


Alternative media, B.sphericus, Anopheles sp.


Some materials can be used to suppress mosquito larvae. One is entopathogenic microbial-based biopesticides, such as Bacillus thuringiensis for the control of Aedes aegypti larvae and Bacillus sphaericus to suppress Culex and Anopheles larvae. B.sphaericus does not require carbohydrates in its growth, because it lacks the enzymes necessary to enter carbohydrates and convert them into energy sources. However, various materials rich in protein and fat are reported to support their growth, even from simple sources. Coconut milk without water, fish flour and soy milk are common food ingredients consumed by the people of Indonesia. The purpose of this research is to know the ability of simple media with 30% v / v coconut milk formula, 30% v / v soy milk, 30% w / v fish flour as a simple medium for B. sphaericus growth. The results showed that a simple medium with a formula of 30% coconut milk showed the most fertile B. sphaericus growth of 296 x 108 cells / ml, fish flour  30% w / v 291 x 108 cells / ml and soy milk 30% v / v 89 x 108 Cell / ml. The results of Bioassay B. sphaericus test on coconut milk media, Anopheles sp larvae that died from 24 hours 15 larvae, at 42 hours and 72 hours killed 20 larvae.




. Arapinis, C., de la Torre, F., & Szulmajster, J. 1988. Nucleotide and deduced amino acid sequence of Bacillus sphaericus 1593M gene encoding a 51.4 kD polypeptide which act synergistically with the 42 kD protein for the expression of the larvicidal toxin. Nucleic Acid Res. 16:7731-7739.

. Baumann P., Clark, M. A., Baumann, L., Broadwell A. H. 1991. Bacillus sphaericus as a mosquito patogen: properties of the organism and its toxin. Microbiol Rev. 55: 425-436.

. Berry, C., Hindley, J., Ehrhardt, A. F., Grounds, T., & de Souza, E. 1993. Genetic determinants of host ranges of Bacillus sphaericus larvacidal toxins. J. Bacteriol. 175:510-518.

. California Department of Public Health. 2008. Overview of Mosquito Control Practices in California. Vector-Borne Disease Section, California Department of Public Health. USA.

. Davidson, E. W. 1988. Binding of the Bacillus sphaericus (Eubacteriales: Bacillaceae) toxin to midgut cells of mosquito (Diptera: Culicidae) larvae: relationship to host range. J. Medical. Entomol. 21:151-157.

. Dulmage, T, A A Yousten, S Singer, and L A Lacey. 1990. Guidelines for Production of Bacillus Thuringiensis H-14 and Bacillus Sphaerirus. Texas, USA: WHO Special Programme for Reasearch and Training in Tropical Disease (TDR).

. Hanafiah KA, M.S. 2011. Experimental design theory and Application. Rajawali Pers, Jakarta.

. Hu, X, W Fan, B Han, H Liu, D Zheng, Q Li, W Dong, et al. 2008. Complete Genome Sequence of the Mosquitocidal Bacterium Bacillus Sphaericus C3-41 and Comparison with Those of Closely Related Bacillus Species. Journal of Bacteriology 190 (8): 2892–2902

. Kalfon, A., Charles, J. F., Bourgoin, C. & de Barjac, H. 1984. Sporulation of Bacillus sphaericus 2297: an electron microsope study of crystal-like inclusion, biogenesis and toxicity to mosquito larvae. J Gen Microbiol. 130:893-900.

. Minitab 17 Support. 2015. Probit Analysis. accessed on 22 February 2016.

. Murray. 2005. Textbook of Microbiology. Publishers of EGC Medical Books, Jakarta.

. Myers, M. & Yousten, A. A. 1978. Toxic activity of Bacillus sphaericus SSII-1 for mosquito larvae. Infect. Immun. 19:1047-1053.

. Suryadi, B. F., B. Yanuwiadi, T. Ardyati, and Suharjono. 2016. “Evaluation of Entomopathogenic Bacillus sphaericus Isolated from Lombok Beach Area against Mosquito Larvae.†Asian Pacific Journal of Tropical Biomedicine 6 (2):148–54.

. Yadav, K, S Dhiman, I Baruah, and L Singh. 2011. “Development of Cost Effective Medium for Production of Bacillus Sphaericus Strain Isolated from Assam, India.†Microbiology Journal 1 (2): 65–70.

. Yousten, A. A., Fretz, S. B. and Jelley, S. A. 1985. Selective Medium for Mosquito-Patogenic Strains of Bacillus sphaericus. Applied and Environmental Microbiology. 49 (6): 1532–33.




How to Cite

Fikri, Z., Jiwintarum, Y., & Diarti, M. W. (2017). Coconut Milk, Soy Milk, and Fish Flour as <i>Bacillus sphaericus’s </i>Alternative Media Material Used in the Control of <i>Anopheles sp.</i>. Asian Journal of Applied Sciences, 5(5). Retrieved from