Coconut Milk, Soy Milk, and Fish Flour as <i>Bacillus sphaericusâ€™s </i>Alternative Media Material Used in the Control of <i>Anopheles sp.</i>
Keywords:Alternative media, B.sphericus, Anopheles sp.
Some materials can be used to suppress mosquito larvae. One is entopathogenic microbial-based biopesticides, such as Bacillus thuringiensis for the control of Aedes aegypti larvae and Bacillus sphaericus to suppress Culex and Anopheles larvae. B.sphaericus does not require carbohydrates in its growth, because it lacks the enzymes necessary to enter carbohydrates and convert them into energy sources. However, various materials rich in protein and fat are reported to support their growth, even from simple sources. Coconut milk without water, fish flour and soy milk are common food ingredients consumed by the people of Indonesia. The purpose of this research is to know the ability of simple media with 30% v / v coconut milk formula, 30% v / v soy milk, 30% w / v fish flour as a simple medium for B. sphaericus growth. The results showed that a simple medium with a formula of 30% coconut milk showed the most fertile B. sphaericus growth of 296 x 108 cells / ml, fish flourÂ 30% w / v 291 x 108 cells / ml and soy milk 30% v / v 89 x 108 Cell / ml. The results of Bioassay B. sphaericus test on coconut milk media, Anopheles sp larvae that died from 24 hours 15 larvae, at 42 hours and 72 hours killed 20 larvae.
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