Oocyte Activation By Calcium Ionophore A23187 in Intracytoplasmic Sperm Injection


  • Abd El Wahab El Ghareeb Zoology Department, Faculty of science, Cairo University, Cairo
  • Ibrahim Yehia Zoology Department, Faculty of science, Cairo University, Cairo
  • Amr Al Ahwani Andrology department, Faculty of Medicine, Cairo University
  • Aziza Mansour International Islamic center for population studies and research;Al-Azhar university
  • Ola Hassan International Islamic center for population studies and research;Al-Azhar university


Calcium ionophore A23187, fertilization rate, embryos quality, oocyte activation


The present study evaluated the effect of calcium ionophore A23187 on oocytes in intracytoplasmic sperm injection (ICSI) cycles using sperm from different sources. The 80 cycles evaluated were divided into four subgroups according to sperm quality and origin (normospermia- oligoathenoteratospermia – epidermal –testicular), subgroups were further split into experimental groups, depending on whether or not treated with calcium ionophore. For each experimental subgroup, ICSI outcomes were compared between groups. In conclusion we found that treatment the oocyts with calcium ionophore increase the fertilization rate in oligoathenoteratospermia, epididymal (PESA) & testicular (TESE) patients without any effect on the embryos quality.



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How to Cite

El Ghareeb, A. E. W., Yehia, I., Al Ahwani, A., Mansour, A., & Hassan, O. (2016). Oocyte Activation By Calcium Ionophore A23187 in Intracytoplasmic Sperm Injection. Asian Journal of Applied Sciences, 4(4). Retrieved from https://ajouronline.com/index.php/AJAS/article/view/4099




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