The Effect of Extrusion Voltage and Flowrate to the Viability and Survivability of Probiotic <em>L. casei</em> Encapsulated in Alginate-Chitosan

Authors

  • Djaenudin Research Unit For Clean Technology, Indonesian Institute of Sciences, Indonesia
  • Endang Saepudin Department of Chemistry, University of Indonesia, Indonesia
  • Muhamad Nasir Department of Chemistry, University of Indonesia, Indonesia

DOI:

https://doi.org/10.24203/ajas.v9i3.6594

Keywords:

Encapsulation, probiotic, L.casei, na-alginate, chitosan, extrusion

Abstract

 Chitosan-coated L. casei containing alginate capsules (shortened as L. casei capsules) were prepared by extruding L. casei containing alginate solution at different extrusion voltage and and flow rate followed by coating the wet capsules in chitosan solution. This study aimed to determine the effect of extrusion voltage and sodium alginate liquid flow rate on the viability of L. casei bacteria in the encapsulation process. The encapsulation process in this study was carried out by the extrusion method using sodium alginate of 1% (w/v) and chitosan of 0.2% (w/v). The resulted beads were immersed in a simulated gastric fluid (SGF) (NaCl 0.2%; HCl 0.5 M with a pH of 1.5) for 1, 60, and 120 min at 37 °C. The number of L. casei cells before encapsulation was 12.3 log CFU. After encapsulation, the maximum viability of L. Casei obtained by voltage variations of 0 kV and flow rate 5 mL/min were 12.26 log CFU.  After testing the beads in SGF for 1 min, the results obtained indicate that viability of L.casei in the sodium alginate - chitosan beads with an extrusion voltage of 0 kV and 5 mL/min was 11.8 log CFU/g. The result indicated that encapsulated L. casei in the sodium alginate - chitosan beads with a voltage of 0 kV and 5 mL/min was the highest survivability level of 97.38 %. The conclusions of the study were The higher extrusion voltage can kill more L. casei while the higher extrusion flow rate can protect more L. casei.

Author Biography

Djaenudin, Research Unit For Clean Technology, Indonesian Institute of Sciences, Indonesia

Research Unit For Clean Technology

References

J. Fao, W. H. O. Working, G. Report, D. Guidelines, and F. London, 2002, Guidelines for the Evaluation of Probiotics in Food.

C. R. Soccol et al., 2010, The potential of probiotics: A review, Food Technol. Biotechnol., 48 (4), 413–434.

R. D. C. S. Ranadheera, S. K. Baines, and M. C. Adams, 2010, Importance of food in probiotic efficacy, Food Research International, 43 (1), 1–7, doi: 10.1016/j.foodres.2009.09.009.

F. C. Prado, J. L. Parada, A. Pandey, and C. R. Soccol, 2008, Trends in non-dairy probiotic beverages, Food Res. Int., 41 (2), 111–123, doi: 10.1016/j.foodres.2007.10.010.

S. Soodbakhsh, H. R. Gheisari, M. Aminlari, and T. Dehnavi, 2012, Viability of encapsulated Lactobacillus casei and Bifidobacterium Lactis in synbiotic frozen yoghurt and their survival under in vitro simulated gastrointestinal conditions, Int. J. Probiotics Prebiotics, 7 (3), 2012.

F. Ortakci and S. Sert, 2012, Stability of free and encapsulated Lactobacillus acidophilus ATCC 4356 in yogurt and in an artificial human gastric digestion system, J. Dairy Sci., 95 (12), 6918–6925, doi: 10.3168/jds.2012-5710.

S. Abbaszadeh, H. Gandomi, A. Misaghi, S. Bokaei, and N. Noori, 2014, The effect of alginate and chitosan concentrations on some properties of chitosan-coated alginate beads and survivability of encapsulated Lactobacillus rhamnosus in simulated gastrointestinal conditions and during heat processing, J. Sci. Food Agric., 94 (11), 2210–2216, doi: 10.1002/jsfa.6541.

W. Krasaekoopt, B. Bhandari, and H. Deeth, 2004, The influence of coating materials on some properties of alginate beads and survivability of microencapsulated probiotic bacteria, Int. Dairy J., 14, 737–743, doi: 10.1016/j.idairyj.2004.01.004.

G. Orive et al., 2003, Cell encapsulation: promise and progress, Nature Medicine, 9 (1), 104–107, doi: 10.1038/nm0103-104.

S. Gouin, 2004, Microencapsulation: Industrial appraisal of existing technologies and trends, Trends Food Sci. Technol., 15 (7–8), 330–347, doi: 10.1016/j.tifs.2003.10.005.

A.M. Mortazavian et al., 2008, Survival of encapsulated probiotic bacteria in Iranian yogurt drink ( Doogh ) after the product exposure to simulated gastrointestinal conditions, Probiotic Bact. Doogh, 63 (4), 349–472.

H. K. Solanki et al., 2013, Development of microencapsulation delivery system for long-term preservation of probiotics as biotherapeutics agent, Biomed Res. Int., 2013, 1–21, doi: https://doi.org/10.1155/2013/620719.

C. Desmond, C. Stanton, G. F. Fitzgerald, K. Collins, and R. Paul Ross, 2002, Environmental adaptation of probiotic lactobacilli towards improvement of performance during spray drying, Int. Dairy J., 12 (2–3), 183–190, doi: 10.1016/S0958-6946(02)00040-7.

E. Mardliyati, S. El Muttaqien, and D. R. Setyawati, 2012, Sintesis nanopartikel kitosan- trypoly phosphate dengan metode gelasi ionik : pengaruh konsentrasi dan rasio volume terhadap karakteristik partikel, Pros. Pertem. Ilm. Ilmu Pengetah. dan Teknol. Bahan, 90–93.

W. Krasaekoopt, B. Bhandari, and H. Deeth, 2003, Evaluation of encapsulation techniques of probiotics for yoghurt, Int. Dairy J., 13 (1), 3–13, doi: 10.1016/S0958-6946(02)00155-3.

J. Burgain, C. Gaiani, M. Linder, and J. Scher, 2011, Encapsulation of probiotic living cells: From laboratory scale to industrial applications, J. Food Eng., 104 (4), 467–483, doi: 10.1016/j.jfoodeng.2010.12.031.

S. Woraharn, C. Chaiyasut, B. Sirithunyalug, and J. Sirithunyalug, 2010, Survival enhancement of probiotic Lactobacillus plantarum CMU-FP002 by granulation and encapsulation techniques, African J. Microbiol. Res., 4 ( 20), 2086–2093.

M. Ali, K. Zanjani, and B. Ghiassi, 2014, Microencapsulation of probiotics by calcium alginate-gelatinized starch with chitosan coating and evaluation of survival in simulated human gastro-intestinal condition, Iran. J. Pharm. Res., 13 (3), 843–852.

S. Nualkaekul, D. Lenton, M. T. Cook, V. V. Khutoryanskiy, and D. Charalampopoulos, 2012, Chitosan coated alginate beads for the survival of microencapsulated Lactobacillus plantarum in pomegranate juice, Carbohydr. Polym., 90 (3), 1281–1287, doi: https://doi.org/10.1016/j.carbpol.2012.06.073.

M. T. Cook, G. Tzortzis, V. V Khutoryanskiy, and D. Charalampopoulos, 2013, chitosan – alginate for the improved survival and administration †, J. Mater. Chem. B, 1, 52–60, doi: https://doi.org/10.1039/c2tb00126h.

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Published

2021-07-01

How to Cite

Djaenudin, Saepudin, E., & Nasir, M. (2021). The Effect of Extrusion Voltage and Flowrate to the Viability and Survivability of Probiotic <em>L. casei</em> Encapsulated in Alginate-Chitosan . Asian Journal of Applied Sciences, 9(3). https://doi.org/10.24203/ajas.v9i3.6594

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