Changes in Chemical Compositions and Enzymatic Activities During Fruit Ripening in Hawthorn <i>(Crataegus Pinnatifida)</i>


  • Jiang-Lian Duan
  • Xiao-Li Ma
  • Guang-Tao Meng
  • Jian-Guo Xu Shanxi Normal University


Chemical compositions, Enzymatic activities, Hawthorn, Ripening


In this study, changes in some chemical compositions associated with fruit quality and enzymatic activities were investigated during ripening of hawthorn fruits from the 88th to 148th day after full bloom day (from August 11 to October 10, 2013). Significant differences in these indices were found between different maturation stages as well as hawthorn cultivars. During ripening of hawthorn fruits, the content of total soluble solids (TSS) and the reducing sugar (RS) increased continuously and progressively. The pH values of hawthorns first decreased significantly and then increased. The activities of polyphenoloxidase (PPO) and peroxidase (POD) in hawthorns decreased significantly during ripening. The catalase (CAT) and phenylalanine ammonia lyase (PAL) activity in Mopan hawthorns decreased significantly, while they first decreased significantly and then increased during ripening of Dajinxing hawthorns. The outcomes of this study provide additional and useful information for fresh consumption and processing as well as utilization of dropped unripe hawthorn fruits.


Agarwal S, Pandey, V. 2004. Antioxidant enzyme responses to NaCl stress in Cassia angustifolia. Biology Plant 48: 555–560.

Alasalvar C, Grigor JM, Zhang D, Quantick PC, Shahidi F. 2001. Comparison of volatiles, phenolics, sugars, antioxidant vitamins, and sensory quality of different colored carrot varieties. Journal of Agricultural and Food Chemistry 49: 1410–1416.

André CM, Schafleitner R, Legay S, Lefèvre I, Aliaga CAA, Nomberto G. 2009. Gene expression changes related to the production of phenolic compounds in potato tubers grown under drought stress. Phytochemistry 70: 1107–1116.

Awad MA, Al-Qurashi AD, Mohamed SA. 2011. Antioxidant capacity, antioxidant compounds and antioxidant enzyme activities in five date cultivars during development and ripening. Scientia Horticulturae 129: 688–693.

Bahorun T, Aumjaud E, Ramphul H, Rycha M, Luximon-Ramma A, Trotin F, Aruoma OI. 2003. Phenolic constituents and antioxidant capacities of Crataegus monogyna (hawthorn) callus extracts. Nahrung 47: 191–198.

Bergmeyer HU, 1974. In: Bergmeyer, H.U. (Ed.), Methods of Enzymatic Analysis, vol. 1, 2nd ed. Academic press, New York, p. 438.

Cao GY, Feng YX, Qin XQ. 1995. Analysis of the chemical constituents of hawthorn fruits and their quality evaluation, Acta Pharmacologica Sinica 30: 138–143.

Chai W-M, Chen C-M, Gao Y-S, Feng H-L, Ding Y-M, Shi Y, Zhou H-T, Chen Q-X. 2014. Structural analysis of proanthocyanidins isolated from fruit stone of Chinese hawthorn with potent antityrosinase and antioxidant activity. Journal of Agricultural and Food Chemistry 62: 123−129.

Chang W, Dao J, Shao, Z. 2005. Hawthorn: potential roles in cardiovascular disease. American Journal of Chinese Medicine 33: 1–10.

Chen SX, Schopfer P. 1999. Hydroxyl-radical production in physiological reactions. A novel function of peroxidase. European Journal of Biochemistry 260: 726-735.

Cui T, Li JZ, Kayahara H, Ma L, Wu LX, Nakamura K. 2006. Quantification of the polyphenols and triterpene acids in Chinese hawthorn fruit by high-performance liquid chromatography. Journal of Agricultural and Food Chemistry 54: 4574–4581.

Distefano G, Casas GL, Caruso M, Todaro A, Rapisarda P, Malfa SL, Gentile A, Tribulato E. 2009. Physiological and molecular analysis of the maturation process in fruits of clementine mandarin and one of its late-ripening mutants. Journal of Agricultural and Food Chemistry 57: 7974–7982.

Huang C-C, Chiang P-Y, Chen Y-Y, Wang CCR. 2007. Chemical compositions and enzyme activity changes occurring in yam (Dioscorea alata L.) tubers during growth. LWT - Food Science and Technology 40:1498–1506

Jiang Y, Duan X, Joyce D, Zhang Z, Li J. 2004. Advances in understanding of enzymatic browning in harvested litchi fruit. Food Chemistry 88: 443–446.

Jiang YM, Zhang ZQ, Joyce DC, Ketsa S. 2002. Postharvest biology and handling of longan fruit (Dimocarpus longan Lour.). Postharvest Biology and Technology 26: 241–252.

Jemai H, Bouaziz M, Sayadi S. 2009. Phenolic composition, sugar contents and antioxidant activity of Tunisian sweet olive cultivar with regard to fruit ripening. Journal of Agricultural and Food Chemistry 57: 2961–2968.

Johnson JL, Bomser JA, Scheerens JC, Giusti MM. 2011. Effect of black raspberry (Rubus occidentalis L.) extract variation conditioned by cultivar, production site, and fruit maturity stage on colon cancer cell proliferation. Journal of Agricultural and Food Chemistry 59: 1638–1645.

Kao E, Wang C, Lin W, Yin Y, Wang C, Tseng T. 2005. Antiinflammatory potential of flavonoid contents from dried fruit of Crataegus pinnatifida in vitro and in vivo. Journal of Agricultural and Food Chemistry 53: 430–436.

Kirakosyan A, Seymour E, Kaufman PB, Warber S, Bolling S, Chang SC. 2003. Antioxidant capacity of polyphenolic extracts from leaves of Crataegus laevigata and Crataegus monogyna (hawthorn) subjected to drought and cold stress. Journal of Agricultural and Food Chemistry 51: 3973-3976

Kulkarni AP, Aradhya SM. 2005. Chemical changes and antioxidant activity in pomegranate arils during fruit development. Food Chemistry 93: 319–324.

Lee SK, Kader AA. 2000. Preharvest and postharvest factors influencing vitamin C content of horticultural crops. Postharvest Biology and Technology 20: 207–220.

Levine A, Tenhaken R, Dixon R, Lamb C. 1994. H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response. Cell 79: 583-593.

Liu P, Kallio H, Yang B. 2011. Phenolic compounds in hawthorn (Crataegus grayana) fruits and leaves and changes during fruit ripening. Journal of Agricultural and Food Chemistry 59: 11141–11149.

Liu P, Kallio H, Lv D, Zhou C, Ou S, Yang B. 2010. Acids, sugars, and sugar alcohols in Chinese hawthorn (Crataegus spp.) fruits. Journal of Agricultural and Food Chemistry 58: 1012–1019.

López-Miranda S, Hernández-Sánchez P, Serrano-Martínez A, Hellín P, Fenoll J, Núñez-Delicado E. 2011. Effect of ripening on protein content and enzymatic activity of Crimson Seedless table grape. Food Chemistry 127: 481–486;

Menz G, Vriesekoop F. 2010. Physical and chemical changes during the maturation of gordal sevillana olives (Olea europaea L., cv. Gordal Sevillana). Journal of Agricultural and Food Chemistry 58: 4934–4938.

Miranda MV., Fernandez Lahor HM., Cascone O. 1995. Horseradish peroxidase extraction and purification by aqueous two-phase partition. Applied Biochemistry and Biotechnology 53: 147–154.

Nokthai P, Lee VS, Shank L. 2010. Molecular modeling of peroxidase and polyphenol oxidase: substrate specificity and active site comparison. International Journal of Molecular Sciences 11(9): 3266–3276.

Opara UL, Al-Ani MR, Al-Rahbi NM. 2012. Effect of fruit ripening stage on physico-chemical properties, nutritional composition and antioxidant components of tomato (Lycopersicum esculentum) cultivars. Food and Bioprocess Technology 5: 3236–3243.

Özcan M, Hacıseferoğulları H, Marakoğlu T, Arslan D. 2005. Hawthorn (Crataegus spp.) fruit: some physical and chemical properties. Journal of Food Engineering 69: 409–413.

Pina A, Errea P. 2008. Differential induction of phenylalanine ammonoia-lyase gene expression in response to in vitro callus unions of prunus spp. Plant Physiology 165: 705–714.

Pittler MH, Schmidt K, Ernst E. 2003. Hawthorn extract for treating chronic heart failure: meta-analysis of randomized trials. American Journal of Medicine 114: 665–674.

Qi X, Li Z, Xu S. 2005. Relationship between soluble sugars and peel pigments in hawthorn fruit. Journal of Fruit Science 22 (1): 81–83 (in Chinese).

Quettier-Deleu C, Voiselle G, Fruchart JC, Duriez P, Teissier E, Bailleul F, Vasseur J, Trotin F. 2003. Hawthorn extracts inhibit LDL oxidation. Pharmazie 58: 577–581.

Rabinowitch HD, Sklan D, Budowski P. 1982. Photooxidative damage in the ripening tomato fruit: protective role of superoxide dismutase. Physiologia Plantarum 54: 369-374.

Rogiers SY, Kumar GNM, Knowles NR. 1998. Maturation and ripening of fruit of Amelanchier alnifolia Nutt. are accompanied by increasing oxidative stress. Annals of Botany 81: 203-211

Scheerens J. Temperate fruit and nut crops. In Hartmann’s Plant Science, 4th ed.; McMahon, M., Ed.; Prentice Hall: Upper Saddle River, NJ, 2006; pp 368–410.

Wang SY, Jiao H. 2001. Changes in Oxygen-Scavenging Systems and Membrane Lipid Peroxidation during Maturation and Ripening in Blackberry. Journal of Agricultural and Food Chemistry 49: 1612-1619

Venkatachalam K, Meenune M. 2012. Changes in physiochemical quality and browning related enzyme activity of longkong fruit during four different weeks of on-tree maturation. Food Chemistry 131: 1437–1442

Wang T, An Y, Zhao C, Han L, Boakye-Yiadom M, Wang W, Zhang Y. 2011. Regulation effects of Crataegus pinnatifida leaf on glucose and lipids metabolism. Journal of Agricultural and Food Chemistry 59: 4987–4994.

Zheng H.-Z, Kim Y-I, Chung S-K. 2012. A profile of physicochemical and antioxidant changes during fruit growth for the utilisation of unripe apples. Food Chemistry 131: 106–110.




How to Cite

Duan, J.-L., Ma, X.-L., Meng, G.-T., & Xu, J.-G. (2014). Changes in Chemical Compositions and Enzymatic Activities During Fruit Ripening in Hawthorn <i>(Crataegus Pinnatifida)</i>. Asian Journal of Agriculture and Food Sciences, 2(5). Retrieved from