Effects of Extraction Methods on Antioxidant Activities of Polysaccharides from the <i>Curcuma phaeocaulis </i>Rhizomes
Keywords:
Curcuma phaeocaulis Velton, Polysaccharides, Extraction, Antioxidant activitiesAbstract
The polysaccharides were extracted from the Curcuma phaeocaulis rhizomes by hot water extraction, ultrasonic-assisted extraction, microwave-assisted extraction and enzyme extraction. The physicochemical properties of C. phaeocaulis polysaccharides were determined by chemical composition analysis. Further, the antioxidant activities were studied via different methods in vitro, including DPPH assay, hydroxyl radical assay and superoxide anion radical assay. The results of the physicochemical property assay showed that the carbohydrate content and uronic acid content of CPP-H, CPP-U, CPP-M, and CPP-E were different, and the protein content was similar. Antioxidant assay indicated that four polysaccharides exhibited significant antioxidant activities in a dose-dependent manner.
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