Study of Anti-nutritional Compounds, Antioxidant Activity and Fatty Acid Composition of Moringa (Moringa oleifera Lam.) Foliage

Authors

  • Nasrin Sultana Bangladesh Livestock Research Institute, Savar, Dhaka -1341
  • Abd Razak Alimon University Putra Malaysia, Serdang 43400, Selangor, Malaysia
  • Khan Shahidul Huque Bangladesh Livestock Research Institute Savar, Dhaka-1341
  • Awis Qurni Sazili University Putra Malaysia, Serdang 43400, Selangor, Malaysia
  • Halimatun Yaakub University Putra Malaysia, Serdang 43400, Selangor, Malaysia
  • S. Mohammad Jahangir Hossain Bangladesh Livestock Research Institute Savar, Dhaka-1341
  • Nani Gopal Das Bangladesh Livestock Research Institute, Savar, Dhaka

Keywords:

Moringa oliefera, anti-nutritional factors, antioxidant activity, fatty acid composition

Abstract

The study was conducted to investigate the effects of cutting intervals on the anti-nutritional factors, antioxidant activity and fatty acid composition of Moringa (Moringa oleifera Lam.) foliage. An established Moringa plot dividing into 12 equal plots was subjected to 3 different maturity stages of harvesting at 4, 6 and 8 weeks in a completely randomized block design experiment. It was found that the level of total phenol and tannin (mg tannic acid equivalent/g dry weight) at 4 (51.86 and 34.90), 6 (43.89, and 27.96) and 8 (29.00 and 16.66) weeks of maturity decreased significantly. Similarly, with the increase of maturity the level of condensed tannin significantly decreased (0.23, 0.17 and 0.14 mg catechin equivalent/g dry weight, respectively). In the case of antioxidant activity, significantly higher DPPH inhibition activity was found after 4 weeks (60.1 %) compared to 6 and 8 weeks of maturity (56.0 and 53.4 %, respectively). However, the fatty acid composition of Moringa foliage was significantly affected with harvesting stage of maturity. It contained the highest level of α-linolenic acid followed by palmitic acid, linoleic acid, stearic acid and oleic acid, respectively (48.71, 21.65, 13.07, 5.89 and 4.63 % of the total identified fatty acids, respectively). The average level of poly unsaturated fatty acid (PUFA) (61.78 %) was about three times higher than the level of saturated fatty acids (31.24 %). In conclusion, harvesting of Moringa foliage at 8 weeks interval would have less anti-nutritional factors with more PUFA and antioxidant activities.

 

Author Biographies

  • Nasrin Sultana, Bangladesh Livestock Research Institute, Savar, Dhaka -1341
    Principal Scientific Officer, Training Planning and Technology Transfer Division
  • Abd Razak Alimon, University Putra Malaysia, Serdang 43400, Selangor, Malaysia
    Professor, Department of Animal Science, Faculty of Agriculture
  • Khan Shahidul Huque, Bangladesh Livestock Research Institute Savar, Dhaka-1341
    Chief Scientific Officer, Animal Production Research Division
  • Awis Qurni Sazili, University Putra Malaysia, Serdang 43400, Selangor, Malaysia
    Professor, Department of Animal Science, Faculty of Agriculture
  • Halimatun Yaakub, University Putra Malaysia, Serdang 43400, Selangor, Malaysia
    Professor, Department of Animal Science, Faculty of Agriculture
  • S. Mohammad Jahangir Hossain, Bangladesh Livestock Research Institute Savar, Dhaka-1341
    Principal Scientific Officer, Biotechnology Division
  • Nani Gopal Das, Bangladesh Livestock Research Institute, Savar, Dhaka
    Scientific Officer, Animal Production Research Division

References

Aye, P.A.,(2007). Production of multinutrient blocks for ruminant and alcohol from the waste products of Leucaena leucocephala and Gliricidia sepium leaves using local technologies. PhD thesis in the Department of Animal Production and Health, Federal University of Technology, Akure, Nigeria.

Asaolu, V.O., Binuomote, R., Akinlade, J.A., Oyelami, O.S., and Kolapo, K.O., (2011). Utilization of Moringa oleifera fodder combinations with Leucaena leucocephala, and Gliricidia sepium fodders by West African Dwarf Goats. International Journal of Agricultural Research, 6: 607-619.

Jabbar, M.A., Reynold, L., Larbi, A. and Smith, J., (1997). Nutritional and economic benefits of Leucaena and gliricidia as feed supplements for small ruminants in humid West Africa. Tropical Animal Health and Production, 29: 35-47.

Akinbamijo, O.O., Adediran, S.A, Nouala, S. and Seeker, J., (2006). Moringa fodder in ruminant nutrition in the Gambia. Trees for life journal – a forum on beneficial trees and plants. Publication of International Trypanotolerance Centre, Banjul T retrieved on 6/4/2010 at HHP://www.tfjournal.org.

Makkar, H.P.S.,(1993). Anti-nutritional factors in foods for livestock.In Animal Production in Developing Countries, Occational publication No. 16, British Society of Animal Production (Eds M. Gill, E Owen, G.E. Pollott and T.L.J Lawrance). pp. 69-85. Edinburg: British Society of Animal Production.

Sreelatha, S. and Padma.P.R., (2009). Antioxidant activity and total phenolic content of Moringa oliefera leaves in two stages of maturity. Plant Foods and Human Nutrition, 64: 303-311.

Nouman, W., Siddiqui, M.T., Basra, S.M.A., Farooq, H., Zubiar, M. and Gull, T., (2013). Biomass production and nutritional quality of Moringa oleifera as field crop. Turkish Journal of Agriculture and Forestry, 37: 410-419.

Foidle N., Makkar H.P.S. and Becker, K..,(2001).The potential of Moringa oleifera for agricultural and industrial uses. In: proceedings of International Workshop. What Development potential for Moringa Products October 29th to November 2nd 2001. Darussalam, Tanzania. http://www.moringanews.org/actes/foild_en.doc.

Udom., G.N. and Idoing, N.B., (2011). Nutrients and anti-nutritional factors of mixed fodder diets for goats in southern Nigeria. Electronic Journal of Environmental Agricultural and Food Chemistry, 10: 2272-2278.

Redden, R.J., Chen, W. and Rao, P.R., (2005). Chickpea Breeding and Management. United Kingdom: CABI.

Moyo, B., Masika, P.J., Hugo, A. and Muchenje, V., (2011). Nutritional characterization of Moringa (Moringa oleifera Lam.) leaves. African Journal of Biotechnology, 10: 12925-12933.

Fahey, J.W., (2005). Moringa oleifera: A review of the medicinal evidence for its nutritional, therapeutic prophylactic properties. Trees for Life Journal, 1: 1-15.

Anwar, F., Latif, S., Ashraf, M. and Gilani, A.H., (2007). Moringa oleifera: A food plant with multiple medicinal uses. Phytotherapy Research, 21: 17-25.

Siddhuraju, P. and Becker, K., (2003). Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. Journal of Agricultural and Food Chemistry. 51: 2144-2155.

Brisibe, E.A, Umoren, U.E., Brisibe, F., Magalhaes, P.M., Ferreira, J.F.S., Luthria, D., Wu., X. and Prior, R.L., (2009). Nutritional characterization and antioxidant capacity of different tissues of Artemisia annua L. Food Chemistry. 115: 1240-1246.

Bramme, H. J., Antonie, A. H., Kassam, H. T. and van Velthuizen, H. T., (1988). Land resources appraisal of Bangladesh for Agricultural Development. Technical Reports 1–7 FAO/UNDP: (BGD/81/035). Agricultural Development Advisor Project, Dhaka, Bangladesh.

Makkar, H.P.S., (2003).Effects and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin-rich feeds. Small Ruminant Research. 49:241-256.

Porter, L.J., Hrstich, L.N. and Chan, B.G., (1986). The conversion of procyanidins and prodelphinidins to cyaniding and delphinidin. Phytochemistry. 25: 223-230.

Atanassova, M., Georgieva, S. and Ivancheva, K., (2011). Total phenolic and total flavonoid contents, antioxidant capacity and biological contaminants medical herbs. Journal of the University of Chemical Technology and Metallurgy. 46: 81-88.

Sanbongi, C., Osakabe, N., Natsume, M., Takizawa, T., Gomi, S. and Osawa, T., (1998). Antioxidative polyphenols isolated from Theobroma cacao. Journal of Agricultural and Food Chemistry. 4:454-457.

Makkar, H.P.S., Siddhuraju, S. and Becker, K., (2007). Plant secondary metabolites; Vol 393 of Methods in Molecular Biology. New York: Springer.

Shen, Q., Zhang, B., Xu, R., Wang, Y., Ding, X, and Li, P., (2010). Antioxidant activity in vitro of selenium-contained protein from the se-enriched. Bifodobacterium animalis. Anaerobe, 16: 380-386.

Yen, G.C., and Duh, P.D., (1994). Scavenging effect methanolic extracts of peanut hulls on free-radical and active oxygen species. J. Agric. Food Chem, 42: 629-632.

Folch, J., Lees, M. and Sloane Stanley, G.H.,(1957). A simple method for the isolation and purification of total lipids from animal tissues. The Journal of Biological Chemistry. 226: 497-509.

Ebrahimi, M.,(2012). Production of omega-3 polyunsaturated fatty acid-enriched chevon using treated oil palm (Elaeis Guineensis) fronds. PhD Thesis, Universiti Putra Malaysia. Malaysia.

AOAC, (2000). Official Methods of Analysis.17thedn. Association of Official Analytical Chemist.Washington, DC., USA.

SAS, (2007). User’s Guide. 9.2 ed. In SAS Inst. Inc, Cary, NC, USA.

Steel, R.G.D. and Torrie, J.H.,(1980). Principles and Procedures of Statistics: A biometric Approach. 2nded. New York; McGraw-Hill Kogakusha.

Makkar, H.P.S. and Becker, K., (1997). Nutrients and anti quality factors in different morphological parts of the Moring oleifera tree.Journal of Agricultural Science, 128: 311-322.

Aye, P.A.and Adegum, M.K. ,(2013). Chemical Composition and some functional properties of moringa, leucaena and gliricidia leaf meals. Agriculture and Biology Journal of North America. 4: 71-77.

Wiermann, R., (1981). Secondary plant products and cell and tissue differentiation. In: Stumf, P. K., Conn, E.E (Eds.), The Biochemistry of Plants, vol. 7. Academic Press Inc., New York, 85-116.

Iqbal, S. and Bhanger, M., (2006). Effect of season and production location on antioxidant activity of Moringa oleifera leaves grown in Pakistan. Food Chemistry. 76: 69-75.

Landrault, N., Poucheret, P., Ravel, P., Gasc, F., Cros, G. andTeissedre, P.L., (2001). Antioxidant activities and phenolic level of French wines from different varieties and vintages. Journal of Agricultural and Food Chemistry, 49: 3341-3348.

Deepa, V.S., Kumar, P.S., Latha, S., Selvamani, P. and Srinivasan, S., (2009). Antioxidant studies on the ethanolic extract of Commiphora spp. African Journal of Biotechnology, 8: 1630-1636.

Rice-Evans, C., Miller, N. and Paganga, G., (1997). Antioxidant properties of phenolic compounds.Trends in Plant Science, 2:152-159.

Lu, C.D. and Jorgensen, N.A., (1987). Alfalfa saponins affect site and extent of nutrient digestion in ruminants. J. Nutr., 117: 919 – 927

Gupta, K., Barat, G. K., Wagle, D. S. and Chawla, H. K. L., (1989). Nutrient contents and anti-nutritional factors in conventional and non-conventional leafy vegetables. Food Chemistry, 31: 105–116.

Cao, G., Sofic, E. and Prior, R.L., (1996). Antioxidant capacity of tea and common vegetables. Journal of Agricultural and Food Chemistry. 44: 3426-3431.

Sena, L.P., Vanderjagt, D.J., Rivera, C., Tsin, A.T., Muhamadu, I., Muhamadou, O., Millson, M., Pastuszyn, A. and Glew, R.H. 1998. Analysis of nutritional components of eight famine foods of the Republic of Niger. Plants Foods for Human Nutrition, 52; 17-30.

Sánchez-Machado, D.I., Nunez-Gastelum, J.A., Reyes-Moreno, C., Ramirez-Wong, B. and Lopez-Cervantes, J.,(2010). Nutritional quality of edible parts of Moringa oleifera. Food analytical Methods, 3:175-180.

Amaglo, N.K., Bennett, R.N., Curto, R.B.L., Rosa, E.A.S., Turco, V.L., Giuffrida, A., Curto, A.L., Crea, F. and Timpo, G.M., (2010). Profiling selected phytochemicals and nutrients in different tissues of the multipurpose tree Moringa oleifera L., grown in Ghana. Food Chemistry. 122: 1047-1054.

Hoffman, L.C. and Wiklund, E., (2006). Game and vension-meat for modern consumers. Meat Science, 74: 197- 208.

Wood, J.D., Enser, M., Fisher, A.V., Nute, G.R., Sheared P.R., Richardson, R.I., Hughes, S.I. and Whittington, F.M., (2008). Fat deposition, fatty acid composition and meat quality: A review. Meat Science, 78: 343-358.

Khotimchenko, S.V., (2005). Lipids from the marine alge Gracilaria verroscosa.Chemistry of Natural Compounds. 43: 285-288.

Wood, J.D., Richardson, R.I., Nute, G.R., Fisher, A.V., Campo, M.M., Kasapidou, E., Sheared, P.R. and Enser, M., (2003). Effect of fatty acids on meat quality: a review. Meat Science, 66: 21-32.

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2017-07-04

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Study of Anti-nutritional Compounds, Antioxidant Activity and Fatty Acid Composition of Moringa (Moringa oleifera Lam.) Foliage. (2017). Asian Journal of Agriculture and Food Sciences, 5(3). https://ajouronline.com/index.php/AJAFS/article/view/4874

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