Polyphenol Composition and in Vitro Antioxidant Activity of Prunus Amygdalus as Affected by Sprouting


  • Neeti Sharma Symbiosis School of Biomedical Sciences Symbiosis International University
  • Ajinkya Ashokrao Shitole Symbiosis School of Biomedical Sciences Symbiosis International University
  • Ashwin Vivek Wadaskar Symbiosis School of Biomedical Sciences Symbiosis International University


— Prunus amygdalus, Free radicals, Antioxidant activity, DPPH


The presence of natural antioxidant in plants is well known. Plant phenolics constitute one of the major groups of components that act as primary antioxidant free radical terminators. This paper reports for the first time the change in the antioxidative activity of methanolic extract of Prunus amygdalus as a result of sprouting. The amounts of total phenolics in the solvent extracts (methanol extract) were determined spectrometrically. From the analyses, methanolic extract of the peel/skin only had the highest total phenolic content (193 μg GA/1000 μg extract) and antioxidant activity (37%) using DPPH method. Increasing the concentration of the extracts resulted in increased antioxidant power for the extracts. Finally, a relationship was observed between the antioxidant potential and total phenolic levels of the extract of Prunus amygdalus.


Agunbiade SO, Olanlokun JO. Evaluation of some nutritional characteristics of Indian almond (Prunusamygdalus) nut. Pakistan J Nutr 5:316–318, 2006.

JahanbanEsfahlan A, Jamei R, JahanbanEsfahlan R. The importance of almond (Prunusamygdalus L.) and its byproducts. Food Chem 120: 349-360, 2010.

Chen CY, Milbury PE, Lapsley K, & Blumberg JB. Flavonoids from almond skins are bioavailable and act synergistically with vitamins C and E to enhance hamster and human LDL resistance to oxidation. Journal of Nutrition 135: 1366–1373, 2005.

Jenkins DJ, Kendall CW, Marchie A, Josse AR, Nguyen TH, Faulkner DA et al. Almonds reduce biomarkers of lipid peroxidation in older hyperlipidemic subjects. Journal of Nutrition 138: 908–913, 2008.

King JC, Blumberg J, Ingwersen L, Jenab M, & Tucker KL. Tree nuts and peanuts as components of a healthy diet. Journal of Nutrition 138: 1736S–1740S, 2008.

Bradley WB, Gregory Dolnikowski, Jeffrey B, Blumberg A, C.-Y. Oliver Chen. Polyphenol content and antioxidant activity of California almonds depend on cultivar and harvest year. Food Chemistry 122: 819–825, 2010.

Geleijnse JM, Launer LJ, Hofman A, Pols HA, & Witteman JC. Tea flavonoids may protect against atherosclerosis: The Rotterdam study. Archives of Internal Medicine 159: 2170–2174, 1999.

Hertog MG, Hollman PC, Katan MB, & Kromhout D. Intake of potentially anticarcinogenic flavonoids and their determinants in adults in The Netherlands. Nutrition and Cancer 20: 21–29, 1993.

Keli SO, Hertog MG, Feskens EJ, & Kromhout D. Dietary flavonoids, antioxidant vitamins, and incidence of stroke: The Zutphen study. Archives of Internal Medicine 156: 637–642, 1996.

Yochum L, Kushi LH, Meyer K, & Folsom AR. Dietary flavonoid intake and risk of cardiovascular disease in postmenopausal women. American Journal of Epidemiology 149: 943–949, 1999.

Hatano T, Edamatsu R, Mori A, Fujita Y, Yasuhara T, Yoshida T, Okuda T.. Effects of theinteraction of tannins with co+existing substances. VI. Effects of tannins and relatedpolyphenols on superoxide anion radical, and on 1,1 +diphenyl+pierylhydrazyl radical. Chem Pharm Bull 37: 2016–2021, 1989.

Slinkard K and Singleton VL. Total phenol analyses: automation and comparison with manual methods. Am. J. Enol. Viticult 28: 49-55, 1977.

Bors W, Michel C, Stettmaier K. Structure–activity relationships governing antioxidant capacities of plant polyphenols. Methods Enzymol 335: 166-180, 2001.

Cuvelier ME, Richard H, Berset C. Comparison of the antioxidant activity of some acid phenols: structure–activity relationship. Biosci Biotech Biochem 56: 324-325, 1992.

Harris PJ, Hartley RD. Detection of bound ferulic acid in cellwalls of the gramineae by ultraviolet fluorescent microscopy. Nat 259: 508-510, 1976.

Ozsoy N, Can A, Yanardag R, Akev N. Antioxidant activity of Smilax excelsa leaf extracts. Food Chem. 110: 571-583, 2008.

Harman d. Aging: a theory based on free radical and radiation chemistry. J Gerontol Jul;11(3):298-300, 1956.

Halliwell B. Free radicals, proteins and DNA: oxidative damage versus redox regulation. Biochem Soc Trans Nov;24(4):1023-7, 1996.

Sies H. Oxidative stress: oxidants and antioxidants. Exp Physiol Mar;82(2):291-5, 1997.

Cadenas E and Packer L. Hand Book of Antioxidants. Plenum Publishers, New York. 1996 (eds).

Rumit S, Heena K, Rajal S, Naveen S. Invitro antioxidant activity of roots ofpurprea linn. Int J Pharmacy Pharm Sci; 2(3):30-33, 2010.




How to Cite

Sharma, N., Shitole, A. A., & Wadaskar, A. V. (2014). Polyphenol Composition and in Vitro Antioxidant Activity of Prunus Amygdalus as Affected by Sprouting. Asian Journal of Agriculture and Food Sciences, 2(4). Retrieved from https://ajouronline.com/index.php/AJAFS/article/view/1600