Comparative Analysis of in Vitro Antioxidant and Cytotoxic Activity of Unripe and Ripe Fruits of <em>Solanum sisymbriifolium</em>

Authors

  • Farhana Momen Department of Biochemistry and Molecular Biology, University of Chittagong, Bangladesh
  • Rashu Barua 2Department of Molecular Biochemistry, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • Md. Golam Kabir Department of Biochemistry and Molecular Biology, University of Chittagong, Bangladesh

DOI:

https://doi.org/10.24203/ajafs.v8i6.6447

Keywords:

Solanum sisymbriifolium, Phenolic content, Antioxidant, Cytotoxicity.

Abstract

Solanum sisymbriifolium Lam. (Solanaceae), commonly referred to as Kantikari (Bengali), sticky nightshade, or litchi tomato. Despite, the traditional use of this plant for several ailments, very little is known about the phytochemical and pharmacological content of the edible ripe berries of this medicinal plant. Ethanol and petroleum ether extract of the ripe and unripe fruits of Solanum sisymbriifolium were examined by using standard analytical methods to determine and compare their bioactive chemical constituents, total phenolic content, antioxidant, and cytotoxic potential. Quantitative phytochemical screening showed that the presence of medicinally active secondary metabolites like alkaloids, flavonoids, and saponins were detected in both ripe and unripe fruits. Antioxidant activity and total phenolic content were found to be more pronounced in ripe fruit extract, whereas unripe fruit extracts showed moderate cytotoxic activity. DPPH free radical scavenging method demonstrated that IC50 value of ethanol and petroleum ether extract of ripe fruits were 194.40μg/ml and 436μg/ml, and for unripe fruit the values were 689.82μg/ml and 1411.23μg/ml. In brine shrimp lethality bioassay, the LC50 value of unripe fruits was 230.6559μg/ml for ethanol extract and 314.9227μg/ml for petroleum ether extract. The LC50 value of the ethanol extract of ripe fruits was 456.1021μg/ml and for petroleum ether extract was 1,017.6848μg/ml. The results of this study showed that the litchi tomato not only has the potential to be a rich source of an edible compound due to the presence of profound phytochemical constituents but also can be used as a source of pharmacological references.

References

REFERENCES

Valko, M., Leibfritz, D., Moncol, J., Cronin, M. T., Mazur, M., & Telser, J. Free radicals and antioxidants in normal physiological functions and human disease. The international journal of biochemistry & cell biology; 39(1): 44–84, 2007.

Rahman, K. Studies on free radicals, antioxidants, and co-factors. Clinical interventions in aging, 2(2): 219. 2007.

Barros, L., Cabrita, L., Boas, M. V., Carvalho, A. M., & Ferreira, I. C. Chemical, biochemical and electrochemical assays to evaluate phytochemicals and antioxidant activity of wild plants. Food Chemistry, 127(4): 1600-1608, 2011.

Choi, C. W., Kim, S. C., Hwang, S. S., Choi, B. K., Ahn, H. J., Lee, M. Y., ... & Kim, S. K. Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison. Plant science, 163(6): 1161-116, 2002.

Kähkönen, M. P., Hopia, A. I., Vuorela, H. J., Rauha, J. P., Pihlaja, K., Kujala, T. S., & Heinonen, M. Antioxidant activity of plant extracts containing phenolic compounds. Journal of agricultural and food chemistry, 47(10): 3954-3962, 1999.

Pandey, K. B., & Rizvi, S. I. Plant polyphenols as dietary antioxidants in human health and disease. Oxidative medicine a cellular longevity, 2(5); 270-278, 2009.

Duthie, G. G., Duthie, S. J., & Kyle, J. A. Plant polyphenols in cancer and heart disease: implications as nutritional antioxidants. Nutrition research reviews; 13(1): 79-106, 2000.

KIMURA, Y. New anticancer agents: in vitro and in vivo evaluation of the antitumor and anti-metastatic actions of various compounds isolated from medicinal plants. In Vivo, 19(1): 37-60, 2005.

Balunas, M. J., & Kinghorn, A. D. Drug discovery from medicinal plants. Life sciences, 78(5): 431-441, 2005.

Rahman, S., Hasnat, A., Hasan, C. M., Rashid, M. A., & Ilias, M. Pharmacological evaluation of Bangladeshi medicinal plants—a review. Pharmaceutical biology; 39(1): 1-6, 2001.

Svobodová B, Kubáň V. Solanaceae: A family well-known and still surprising. Bentham Science Publishers; 2018 Nov 15.

Varandas, R., Egas, C., & Conceição, I. Luci. Potato cyst nematodes: New solutions to an old problem. Crop protection; 137: 105303, 2020.

Ferro, E. A., Alvarenga, N. L., Ibarrola, D. A., Hellión-Ibarrola, M. C., & Ravelo, A. G. A new steroidal saponin from Solanum sisymbriifolium roots. Fitoterapia; 76(6): 577-579, 2005.

Weissenberg, M. Isolation of solasodine and other steroidal alkaloids and sapogenins by direct hydrolysis-extraction of Solanum plants or glycosides therefrom. Phytochemistry; 58(3): 501-508, 2001.

Chakravarty, A. K., Mukhopadhyay, S., Saha, S., & Pakrashi, S. C. A neolignan and sterols in fruits of Solanum sisymbri [i] folium (collected from India). Phytochemistry; 41(3): 935-939, 1996

Hellión-lbarrola, M. C., Montalbetti, Y., Heinichen, O., Alvarenga, N., Figueredo, A., & Ferro, E. A. Isolation of hypotensive compounds from Solanum sisymbriifolium Lam. Journal of ethnopharmacology; 70(3): 301-307, 2000.

Barua, R., Sultana, S., Talukder, M. E. U., Chakma, K., Hasan, C. M. M., & Islam, M. S. Antioxidant and cytotoxic activity of crude flavonoid fraction from the fruits of hybrid variety of Momordica charantia (Bitter gourd). Journal of Pharmaceutical Research International; 4(7): 778-786, 2014.

Harborne, J.B., Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 2nd edition, Chapman and Hall Publishers, New York; pp: 85, 1973.

Edeoga, H.O., Okwu, D.E and Mbaebie, B.O. Phytochemical constituents of some Nigerian medicinal plants. Afr. J. Biotechnol; 4(7): 685-688, 2005.

Boham,B.A., & Kocipai-Abyazan, R. Flavonoids and condensed tannins from leaves of Hawaiian vaccinium vaticulatum and V. calycinium. Pacific sci; 48: 458-463. 1974.

Obadoni, B.O. and Ochuko, P.O. Phytochemical studies and comparative efficacy of the crude extracts of some homeostatic plants in Edo and Delta States of Nigeria. Global J. Pure and Appl. Sci; 8:203-208, 2001

Mills, D.F. Symposia from the XII International congress on Nutrition Prog. Clin. Biol. Res; 77: 165 – 71, 1981.

Ozturk M, Ozturk FA, Duru ME, Topcu G. Antioxidant activity of stem and root extracts of Rhubarb (Rheum ribes): An edible medicinal plant. Food Chem; 103:623–630, 2007.

Marxen K, Vanselow KH, Lippemeier S, Hintze R, Ruser A, Hansen UP. Determination of DPPH radical oxidation caused by methanolic extracts of some microalgal species by linear regression analysis of spectrophotometric measurements. Sensors;7:2080-2095, 2007.

Lee YR, Woo KS, Kim KJ, Son JR, Jeong HS. Antioxidant Activities of Ethanol Extracts from Germinated Specialty Rough Rice. Food Sci Biotechnol;16:765- 770, 2007.

Apak, R., Özyürek, M., Güçlü, K., & Çapanoğlu, E. Antioxidant activity/capacity measurement. 1. Classification, physicochemical principles, mechanisms, and electron transfer (ET)-based assays. Journal of agricultural and food chemistry; 64(5): 997-1027, 2016.

Gupta, V. K., Simlai, A., Tiwari, M., Bhattacharya, K., & Roy, A. Phytochemical contents, antimicrobial and antioxidative activities of Solanum sisymbriifolium. Journal of Applied Pharmaceutical Science; 4(3): 75, 2014.

Prakash V, Saxena S, Gupta S, Singh SK. Preliminary phytochemical screening and biological activities of Adina cardifolia. J Microb Biochem Technol. 2015; 7:33–38.

Osman ME, Yassen HH, Deng Deng J, Hussein MS. General Phytochemical Screening and Antioxidant Activity of Some Sudanese Medicinal Plants. J Forest Prod Ind. 2014;3:292–295.

Zhang, H. F., Yang, X. H., & Wang, Y. Microwave assisted extraction of secondary metabolites from plants: Current status and future directions. Trends in Food Science & Technology; 22(12):672-688, 2011.

Gorelick, J., & Bernstein, N. Elicitation: an underutilized tool in the development of medicinal plants as a source of therapeutic secondary metabolites. In Advances in agronomy; 124: 201-230. Academic Press, 2014.

Mohammedi, Z., & Atik, F. Impact of solvent extraction type on total polyphenols content and biological activity from Tamarix aphylla (L.) Karst. International journal of Pharma and Bio science; 2(1): 609-615, 2011

Rice-evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridham JB. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radic Res.; 22(4):375-383, 1995.

Simlai, A., & Roy, A. Analysis of and correlation between phytochemical and antimicrobial constituents of Ceriops decandra, a medicinal mangrove plant, from Indian Sundarban estuary. Journal of Medicinal Plants Research; 6(32): 4755-4765, 2012.

Shulaev, V., Cortes, D., Miller, G., & Mittler, R. Metabolomics for plant stress response. Physiologia plantarum; 132(2): 199-208, 2008.

Reyes, L. F., & Cisneros-Zevallos, L. Wounding stress increases the phenolic content and antioxidant capacity of purple-flesh potatoes (Solanum tuberosum L.). Journal of Agricultural and Food Chemistry; 51(18): 5296-5300, 2003.

Pourmorad, F., Hosseinimehr, S. J., & Shahabimajd, N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African journal of biotechnology; 5(11): 1142-1145, 2006.

Akhila S, Bindu A, Bindu K, Aleykutty N. Comparative evaluation of extracts of Citrus limon burm peel for antioxidant activity. J. Young Pharmacists, 1(2):136, 2009

Bernardi APM, López-Alarcón C, Aspée A, Rech SB, Von Poser GL, Bridi R, Dutrafilho CS, Lissi E. AntioxidantActivity in Southern Brazil Hypericum species. J. Chil. Chem. Soc. 53(4):1658-1662, 2008.

More, G. K. A review of the ethnopharmacology, phytochemistry and pharmacological relevance of the South African weed Solanum sisymbriifolium Lam. (Solanaceae). Environment, Development and Sustainability; 21(1), 37-50, 2019.

Kaur S, Michael H, Arora S, Härkönen PL, Kumar S. The in vitro cytotoxic and apoptotic activity of Triphala—an Indian herbal drug. J Ethnopharmacol;97(1):15-20, 2005.

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Published

2020-12-29

How to Cite

Momen, F. ., Barua, R. ., & Kabir, M. G. (2020). Comparative Analysis of in Vitro Antioxidant and Cytotoxic Activity of Unripe and Ripe Fruits of <em>Solanum sisymbriifolium</em>. Asian Journal of Agriculture and Food Sciences, 8(6). https://doi.org/10.24203/ajafs.v8i6.6447