Occurrence of <i>Alfalfa Mosaic Virus </i>(AMV) Infecting Bean Crop in Burdur Province, Turkey

Authors

  • H. Çulal Kiliç Süleyman Demirel University, Agriculture Faculty, Plant Protection Department Isparta, Turkey
  • Nejla Yardimci Süleyman Demirel University, Agriculture Faculty, Plant Protection Department Isparta, Turkey

Keywords:

Virus, detection, ELISA, IC-RT-PCR

Abstract

During surveys conducted in 2012- 2013 growing season, field-grown bean plants with symptoms of mosaic, chlorotic mottling, vein banding, blistering and leaf malformation were observed. A total of 118 leaf samples were collected from various locations in this region. Alfalfa mosaic virus (AMV), was detected in bean, using enzyme-linked immunosorbent assay (ELISA), and immunocapture-reverse-transcription-polymerase chain reaction (IC-RT-PCR) methods. ELISA tests showed that among 118 field samples tested, 7 were infected with AMV. Seven leaf samples that had tested positive in DAS-ELISA were used for IC-RT-PCR. IC-RT-PCR was carried out by using specific primer which amplified a 351 bp fragment of coat protein of AMV in samples. The presence of AMV in the leaf samples was further confirmed IC-RT-PCR using specific primers.

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Published

2015-04-14

How to Cite

Çulal Kiliç, H., & Yardimci, N. (2015). Occurrence of <i>Alfalfa Mosaic Virus </i>(AMV) Infecting Bean Crop in Burdur Province, Turkey. Asian Journal of Agriculture and Food Sciences, 3(2). Retrieved from https://ajouronline.com/index.php/AJAFS/article/view/2520

Issue

Vol. 3 No. 2 (2015): April 2015

Section

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