Isolation and Identification of a Begomovirus Associated With the Leaf Curl Disease of Gumamela (<i>Hibiscus rosa-sinensis</i>) in the Philippines
Keywords:begomovirus, Cotton leaf curl Multan virus, Hibiscus rosa-sinensis, gumamela leaf curl
Hibiscus rosa-sinensis, locally known as â€˜gumamelaâ€™ were observed with virus symptoms of mosaic, leaf curl, cupping, enation, mottling, and stunting. The virus was transmitted by whiteflies and by grafting to different host plant species including: Gossypium hirsutum, Vigna unguiculata and Hibiscus rosa sinensis but not to Abelmoschus esculentus, Datura metel, D. stramonium, Solanum melongena , Lycopersicon esculentum, Nicotiana glutinosa andÂ Physalis floridana . The thirty four (34) gumamela samples showed positive to the PCR amplification with begomovirus degenerate primers yielding an expected band size of ~1.5kb. Multiple sequence alignment of the obtained DNA-A fragments showed 97.0-99.0% similarities among isolates. Further sequence analysis of Philippine hibiscus leaf curl isolate with other hibiscus leaf curl viruses registered in the Genbank showed 98% nucleotide similarities with the Cotton leaf curl Multan virus.
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