Isolation and Identification of a Begomovirus Associated With the Leaf Curl Disease of Gumamela (Hibiscus rosa-sinensis) in the Philippines
Keywords:
begomovirus, Cotton leaf curl Multan virus, Hibiscus rosa-sinensis, gumamela leaf curlAbstract
Hibiscus rosa-sinensis, locally known as ‘gumamela’ were observed with virus symptoms of mosaic, leaf curl, cupping, enation, mottling, and stunting. The virus was transmitted by whiteflies and by grafting to different host plant species including: Gossypium hirsutum, Vigna unguiculata and Hibiscus rosa sinensis but not to Abelmoschus esculentus, Datura metel, D. stramonium, Solanum melongena , Lycopersicon esculentum, Nicotiana glutinosa and Physalis floridana . The thirty four (34) gumamela samples showed positive to the PCR amplification with begomovirus degenerate primers yielding an expected band size of ~1.5kb. Multiple sequence alignment of the obtained DNA-A fragments showed 97.0-99.0% similarities among isolates. Further sequence analysis of Philippine hibiscus leaf curl isolate with other hibiscus leaf curl viruses registered in the Genbank showed 98% nucleotide similarities with the Cotton leaf curl Multan virus.
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