Isolation and Identification of a Begomovirus Associated With the Leaf Curl Disease of Gumamela (Hibiscus rosa-sinensis) in the Philippines
Keywords:
begomovirus, Cotton leaf curl Multan virus, Hibiscus rosa-sinensis, gumamela leaf curlAbstract
Hibiscus rosa-sinensis, locally known as ‘gumamela’ were observed with virus symptoms of mosaic, leaf curl, cupping, enation, mottling, and stunting. The virus was transmitted by whiteflies and by grafting to different host plant species including: Gossypium hirsutum, Vigna unguiculata and Hibiscus rosa sinensis but not to Abelmoschus esculentus, Datura metel, D. stramonium, Solanum melongena , Lycopersicon esculentum, Nicotiana glutinosa and Physalis floridana . The thirty four (34) gumamela samples showed positive to the PCR amplification with begomovirus degenerate primers yielding an expected band size of ~1.5kb. Multiple sequence alignment of the obtained DNA-A fragments showed 97.0-99.0% similarities among isolates. Further sequence analysis of Philippine hibiscus leaf curl isolate with other hibiscus leaf curl viruses registered in the Genbank showed 98% nucleotide similarities with the Cotton leaf curl Multan virus.
References
Staples, G. W. and Herbst, D.R. A Tropical Garden Flora: Plants cultivated in the Hawaiian Islands and other tropical places. Bishop Museum Press, Honolulu, Hawaii.. 2005.
Magdalita, P.M. and Pimentel, R.B. Development of Hibiscus hybrids ‘ Women in Public Service Series II’ and propagation studies in hibiscus rosa-sinensis. . 2013.
Regenmortel, M.H.V., Fauquet, C.M., Bishop, D.H.L., Carsten, E.B., Estes, M. R., Lemon, S.M., Maniloff, J., Mayo, M., McGeoch, D.J., Pringle, C.R., & Wickner, R.B. Virus Taxonomy. 7th Report of the ICTV, Academic Press, New York.2000.
Mansoor, S, Hussain M, Khan, SH, Bashir A, Leghari AB, Panwar GA, Siddiqui WA, Zafar, Y and Malik, K.A.. Polymerase chain reaction-based detection of cotton leaf curl and other whitefly-transmitted geminiviruses from Sindh. Pak J Biol Sci 1: 39-4. 1998.
Rajeswari Reddy, RVC., Maruthi, MN., Colvin, J., Seal, SE. & Muniyappa, V. Host range, vector relationships and sequence comparison of a begomovirus infecting hibiscus in India. Annals of Applied Biology 147:15-25. 2005.
Mao, M.J., He, Z.F., Yu, H., and Li, H.P. Molecular characterization of cotton leaf curl Multan virus and its satellite
DNA that infects Hibiscus rosa-sinensis. Chinese Journal of Virology 24:64–68. 2008
Dellaporta , SL, Wood, J., Hicks, JB.. A plant DNA minipreparation: Version II. Plant Mol. Bio. 1: 19-21. 1983
Gilbertson, R.I., Rojas, M. R., Russel, D.R.,Maxwell, D.P. Use of asymmetric polymerase chain reaction and DNA
sequencing to determine genetic variability of bean golden mosaic geminivirus ihe Dominican Republic. Journal of
General Virology, 72, 2843-2848. 1991.
Tsai, WS, Shih, SL, Venkatesan, SG, Aquino, MU, Green, SK, Kenyon L and Jan, FJ. Distribution and Genetic iversity
of Begomoviruses infecting Tomato and Pepper Plants in the Philippines. Annals of Applied Biology. ISSN 0004-
2011.
Dolores, L.M and Bajet, N.B. Isolation and transmission of tomato leaf curl virus in the Philippines. Philippine
Phytopathology, 31, 40-51. 1995.
Dolores, L.M and Valdez, R.B. Identification of squash viruses and screening for resistance.. Philippine Phytopathology,
, 43-52. 1988.
Kon, T., Rojas, M.R., Abdourhamane, I.K. and Gilbertson, R. L. Roles and interactions of begomoviruses and satellite DNAs associated with okra leaf curl disease in Mali, west Africa. Journal of General Virology, 90, 1001-1013. 2009.
. 13. Pawar, D.M. and Mahatma, L. Whitefly transmitted Begomovirus in China-rose: A threat to the beauty. International Journal of Plant Protection 6:2, 379-381.2013.
Varma, A. and Malathi, V.G. Emerging geminivirus problems: A serious threat to crop production. Ann Appl Biol
:145-164. 2003.
Moffat, A.S. Geminiviruses emerge as serious crop threat. Science 286: 1835. Doi:10.1126/science.286.5446.1835. 1999.
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