Fungal Endophytes in Sweet Orange <i>Citrus sinensis</i> (L.) Osbeck in Jujuy-Argentina

Authors

  • Noemí del Valle Bejarano Fitopatología. FCA.UNJu.
  • Leonor Carrillo

Keywords:

Endophytes, orange, Xylaria, Phyllosticta, Guignardia

Abstract

According to the interaction established with the plant tissues, the endophytes can be mutualistic or pathogenic fungi. El objetivo del trabajo fue reconocer los endófitos fúngicos de las hojas de naranjo dulce Citrus sinensis (L.) Osbeck en la zona citrícola de Jujuy y estudiar sus interacciones in vitro.The objectives were to identify the fungal endophytes of the sweet orange leaves [Citrus sinensis (L.) Osbeck] from Jujuy-Argentina, and study the in vitro interactions between the isolates.En diferentes sitios de la provincia, se tomaron muestras de hojas asintomáticas del brote más antiguo, se conservaron refrigeradas hasta su procesamiento, se lavaron y desinfectaron. The asymptomatic leaves were taken from the oldest shoots.  Asépticamente se cortaron trozos de hojas que se sembraron en placas con agar zanahoria.Leaf pieces were aseptically plated on agar carrot. Se hicieron observaciones, y determinaciones morfométricas.Isolation, culture and morphometric measurements were made. Para las especies cuya diferenciación morfológica es discutida, la identificación se realizó por PCR-ITS.For some species, identification was performed by PCR-ITS. Se realizaron inoculaciones sobre hojas y frutos de naranjo, y pruebas de antagonismo entre las especies halladas sin potencial patógeno.Inoculations on leaves and fruit orange were carried out, and antagonism test were conducted between non-pathogenic endophytes and the pathogen G. citricarpa.The fungal eLos colonizadores endófitos encontrados fueron: Glomerella cingulata , Lasiodiplodia theobromae , Phomopsis citri , Guignardia citricarpa, Phyllosticta capitelensis (Syn G. mangiferae ) , Cladosporium herbarum, Xylaria hypoxylon, Xylaria multiplex, Xylaria sp., Fusarium sp., Penicillium sp. , Alternaria sp. , Diplococium sp . y Stenella sp.ndophytes isolated from citrus leaves were: Colletotrichum gloeosporioides, Glomerella cingulata, Lasiodiplodia theobromae, Phomopsis citri, Guignardia citricarpa, Phyllosticta capitalensis, Cladosporium herbarum, Xylaria hypoxylon, X. multiplex, Xylaria sp., Fusarium sp., Penicillium sp., Alternaria sp., Diplococium sp.,and Stenella sp. Xylaria spp., C. gloeosporioides and P. capitalensis were found antagonists against G. citricarpa.

Author Biography

Noemí del Valle Bejarano, Fitopatología. FCA.UNJu.

Sanidad Vegetal. Profesor Titular de Fitopatología

References

Alcoba N, Vigiani A, Bejarano N, Alvarez S, Serrano M, Bonillo M. Mancha negra de los cítricos, epidemiología y control. Ediunju. S.S. de Jujuy. Jujuy. Argentina, 2000.

Baayen R, Bonants P, Verkley G, Carroll G, Van Der AA H, Weerdt M, Brouwrshaven GC; Schutte GC, Maccheroni Jr. W, Glienke-Blanco C, Azevedo J., Nonpathogenic isolates of the citrus black spot fungus, Guignardia citricarpa, identified as a cosmopolitan endophyte of woody plants, G. mangiferae (Phyllosticta capitalensis), Phytopathology, vol. 92, pp. 88-92, 2002.

Baldassari R, Wickert E, de Goes A., Pathogenicity, colony morphology and diversity of isolates of Guignardia citricarpa and G. mangiferae isolated from Citrus spp., Eur. J. Plant. Pathol., vol. 120, pp. 103–110, 2008.

Bell DK, Wells HD, Markaman CR. In vitro antagonism of Trichoderma spp. against six fungal pathogens, Phytopathology, vol. 72, pp. 379-382, 1982.

Douanla-Meli C, Langer E, Talontsi Mouafo F., Fungal endophyte diversity and community patterns in healthy and yellowing leaves of Citrus limon, Fungal Ecology vol. 6, pp. 212-222, 2013.

Dugan F, The identification of fungi, APS Press, St. Paul, MN., USA, 2006.

Durán E, Ploper D, Ramallo J, Piccolo-Grandi R, Hupper-Giancoli A, Azevedo J., The foliar fungal endophytes of Citrus limon in Argentina, Can. J. Bot., vol. 83, pp. 350-355, 2005.

Er, H. L., Hendricks, K., Goss, E. M., Smith, M., Schubert, T. S., Roberts, P. D., & van Bruggen, A. H. C., Isolation and biological characterization of Guignardia species from citrus in Florida, Journal of Plant Pathology, vol. 96 (1), pp. 43-55, 2014.

Fogliata G, Canton N, Muñoz M, Ploper L, Farias M, Vellice G. Salgado M, Ontivero M, Castagnaro A., Síntomas de “moteado†en frutos y hojas de limonero en Tucumán causados por Guignardia mangiferae, Avance Agroindustrial, pp. 21-26, 2004.

Fogliata, G. M., Ploper, L. D., Muñoz, M. L., Canton, N., & Acosta, E., Mancha rojiza en frutos de limonero en Tucumán se determinó que no es causada por Guignardia citricarpa, el agente causal de la mancha negra de los cítricos, Avance agroindustrial, vol. 27(4) pp. 23-26, 2006.

Glienke C, Pereira O, Stringari D, Fabris J, Kava-Cordeiro V, Galli-Terasawa L, Cunnington J, Shivas R, Groenewald J, Crous P., Endophytic and pathogenic Phyllosticta species with reference to those associated with citrus black spot, Persoonia, vol. 26, pp. 47-56, 2011.

Hidalgo-Góngora E, Pérez V. Diferenciación morfológica, cultural y biológica de Guignardia citricarpa y G. mangiferae en frutos cítricos de Cuba, Fitosanidad, vol.14, pp.141-152, 2010.

Kupper KC, Barbosa-Correa E, Moretto C, Bettiol W, de Goes A. Control of Guignardia citricarpa by Bacillus subtilis and Trichoderma spp., Rev. Bras. Frutic., vol. 33, pp. 1111-1118, 2011.

Liers, C., Ullrich, R., Steffen, K. T., Hatakka, A., & Hofrichter, M. Mineralization of 14C-labelled synthetic lignin and extracellular enzyme activities of the wood-colonizing ascomycetes Xylaria hypoxylon and Xylaria polymorpha, Applied Microbiology and Biotechnology, vol. 69(5), pp. 573-579, 2006.

Mejia, L. C., Rojas, E. I., Maynard, Z., Van Bael, S. A., Arnold, E. A., Hebbar, P., Endophytic fungi as biocontrol agents of Theobroma cacao pathogens, Biol. Control, vol 46, pp. 4–14.doi:10.1016/j.biocontrol.2008.01.012.

Mejía L C, Herre E A, Sparks J P, Winter K, García M N, Van Bael S A, StittJ, Shi Z, Zhang Y, Guiltinan M J and Maximova S N, Pervasive effects of a domi- nant foliar endophytic fungus on host genetic and phenotypic expression in a tropical tree, Front. Microbiol., vol. 5 : 479. doi:10.3389/fmicb.2014.00479.

Meyer, L., Jacobs, R., Kotzé, J. M., Truter, M., & Korsten, L., Detection and molecular identification protocols for Phyllosticta citricarpa from citrus matter, South African Journal of Science, vol. 108(3-4), pp. 53-59, 2012.

Morales-Rondón V. y Rodríguez-González M., Hongos endófitos en plantaciones de mango “Haden†de la Planicie de Maracaibo, Rev. Fac. Agronomía, vol. 23, pp. 273-284, 2006.

Osono T., Diversity and ecology of endophytic and epiphytic fungi of tree leaves in Japan: a review. En: Verma VC, Gange AC (Ed), Advances in Endophytic Research, New Delhi, Springer, pp. 3-26, 2013.

Rodriguez A, 2007., Caracterización molecular de población de Colletotrichum spp. asociada a Coffea arabica en Colombia y su aplicación en el diagnostico del CBD, Bogotá Pontificia Universidad Javeriana, http://www.javeriana.edu.co/biblos/tesis/ciencias/tesis18.pdf.

Salazar C y de García M. C., Aislamiento de hongos endofitos en rosa (Rosa hybrida) en Bogotá, Colombia, Rev. Iberoam. Micol., vol. 22, pp. 99-101, 2005.

San Martín F, Rogers J., A preliminary account of Xylaria of México, Mycotaxon, vol. 34, pp. 283-373, 1989.

Stammler, G., Schutte, G. C., Speakman, J., Miessner, S., & Crous, P. W., Phyllosticta species on citrus: Risk estimation of resistance to QoI fungicides and identification of species with cytochrome b gene sequences, Crop Protection, vol. 48, pp. 6-12, 2013.

Timmer, L. W., Mondal, S. N., Peres, N. A. R., & Bhatia, A., Fungal diseases of fruit and foliage of citrus trees, In Diseases of Fruits and Vegetables vo.l I (pp. 191-227). Springer Netherlands, 2004

Wickert E, de Goes A, de Macedo-Lemos EG, de Souza A, da Silveira EL, Dias Pereira F, Rinaldo D., Relações filogenéticas e diversidade de isolados de Guignardia spp. oriundos de diferentes hospedeiros nas regiões ITS1-5,8SITS2, Rev. Bras. Frutic., vol. 31, pp. 360–380, 2009.

Wright J, Johnson G, Hyde K., Studies on the endophytic mycota of Citrus spp., Proceding of international workshop holdat Chiang Mia, Thailand, ACIAR Procedings Series, vol. 80, pp. 167-173, 1998.

Wu SH, He J, Li XN, Huang R, Song F, Chen YC, Guaiane sesquiterpenes and isopimarane diterpenes from an endophytic fungus Xylaria sp., Phytochemistry, doi: 10.1016/j.phytochem.2014.04.016.

Zavala, M. G. M., Er, H. L., Goss, E. M., Wang, N. Y., Dewdney, M., & van Bruggen, A. H., Genetic variation among Phyllosticta strains isolated from citrus in Florida that are pathogenic or nonpathogenic to citrus, Tropical Plant Pathology,vol. 39(2), pp. 119-128, 2014.

Downloads

Published

2016-02-19

How to Cite

Bejarano, N. del V., & Carrillo, L. (2016). Fungal Endophytes in Sweet Orange <i>Citrus sinensis</i> (L.) Osbeck in Jujuy-Argentina. Asian Journal of Agriculture and Food Sciences, 4(1). Retrieved from https://ajouronline.com/index.php/AJAFS/article/view/3121

Most read articles by the same author(s)