Chemical Composition and Antibacterial Activity of the Essential Oil from Qiancao (Rubia cordifolia Linn.) Roots against Selected Foodborne Pathogens
DOI:
https://doi.org/10.24203/ajafs.v7i4.5876Keywords:
Rubia cordifolia, chemical composition, antimicrobial activity, essential oilAbstract
Chemical composition of the essential oil from Qiancao roots was analyzed by gas chromatography-mass spectrometry (GC-MS), and 43 components were identified in the paper. Mollugin (30.58%) was found to be the major component, followed by 3-methyl-2-cyclopenten-1-one (10.55%), 4-tert-butyl-2-phenyl-phenol (8.64%) and eugenol (6.30%). The antibacterial activity of essential oil against selected food-borne pathogens was evaluated. The results showed that the sensitivity to essential oil were different for different tested bacteria and the essential oil had a strong antibacterial activity against B. cereus with the lowest minimum inhibitory concentration (MIC) and minimum bactericide concentration (MBC) values of 0.0625 and 0.125 mg/mL, respectively. In addition, microbial growth curve assay also showed that the essential oil had a significant effect on the growth rate of surviving B. cereus. The data of this study suggests that the essential oil from Qiancao roots has great potential for application as a natural antimicrobial agent to preserve food.
References
Diao W-R, Hu Q-P, Zhang H, Xu, J-G. 2014. Chemical composition, antibacterial activity and mechanism of action of essential oil from seeds of fennel (Foeniculum vulgare Mill.). Food Control 35: 109–116.
Divakar K, Pawar AT, Chandrasekhar SB, Dighe SB, Divakar G. 2010. Protective effect of the hydro-alcoholic extract of Rubia cordifolia roots against ethylene glycol induced urolithiasis in rats. Food and Chemical Toxicology 48: 1013–1018.
Gao CY, Tian CR, Lu YH, Xu JG, Luo JY, Guo XP. 2011. Essential oil composition and antimicrobial activity of Sphallerocarpus gracilis seeds against selected food-related bacteria. Food Control 22: 517–522.
Han Y-S, der Heijden RV, Verpoorte R. 2001. Biosynthesis of anthraquinones in cell cultures of Rubiaceae. Plant Cell Tissue and Organ Culture 67: 201–220.
Hussain AI, Anwar F, Nigam PS, Sarker SD, Moore JE, Rao JR, Mazumdar A. 2011. Antibacterial activity of some Lamiaceae essential oils using resazurin as an indicator of cell growth. LWT-Food Science and Technology 44: 1199–1206.
Kaur P, Chandel M, Kumar S, Kumar N, Singh B, Kaur S. 2010. Modulatory role of alizarin from Rubia cordifolia L. against genotoxicity of mutagens. Food and Chemical Toxicology 48: 320–325.
Liu R, Lu YB, Tian X, Pan Y. 2008. Simultaneous isolation and purification of mollugin and two anthraquinones from Rubia cordifolia by HSCCC. Chromatographia 28(1): 95–99.
Lodia S, Kansala L. 2012. Antioxident activity of Rubia cordifolia against lead toxicity. International Journal of Pharmaceutical Science and Reseach 3(7): 2224–2232.
Ma TT, Luo JY, Tian CR, Sun XY, Quan MP, Zheng CP, Kang LN, Zhan JC. 2011. Influence of technical processing units on chemical composition and antimicrobial activity of carrot (Daucus carrot L.) juice essential oil. Food Chemistry 170: 394–400.
Miyazawa M, Kawata J. 2006. Identification of the key aroma compounds in dried roots of Rubia cordifolia. Journal of Oleo Science 1(55): 37–39.
Sarker SD, Nahar L, Kumarasamy Y. 2007. Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods 42: 321–324.
Sokmen A, Gulluce M, Akpulat HA, Daferera D, Tepe B, Polissiou M, Sokmen M, Sahin, F. 2004. The in vitro antimicrobial and antioxidant activities of the essential oils and methanol extracts of endemic Thymus spathulifolius. Food Control 15: 627–634.
s
Downloads
Published
Issue
Section
License
- Papers must be submitted on the understanding that they have not been published elsewhere (except in the form of an abstract or as part of a published lecture, review, or thesis) and are not currently under consideration by another journal published by any other publisher.
- It is also the authors responsibility to ensure that the articles emanating from a particular source are submitted with the necessary approval.
- The authors warrant that the paper is original and that he/she is the author of the paper, except for material that is clearly identified as to its original source, with permission notices from the copyright owners where required.
- The authors ensure that all the references carefully and they are accurate in the text as well as in the list of references (and vice versa).
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Attribution-NonCommercial 4.0 International that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
- The journal/publisher is not responsible for subsequent uses of the work. It is the author's responsibility to bring an infringement action if so desired by the author.
