In Vitro Activity of Hexane and Ethanol Extracts of Camelthorn, Alhagi maurorum against Plant Pathogenic Fungi and Bacteria

Authors

  • Soad M. Ahmed Alexandria University, Alex, Egypt
  • AMal A. Housien
  • Ahmed A. Ismail
  • Farid S. Sabra

Keywords:

Keywords, Alhagi maurorum, Plant pathogenic fungi, Plant pathogenic bacteria, GC- mass spectroscopy.

Abstract

The effeciency of hexane and ethanol extracts of camelthorn, (Alhagi maurorum) was evaluated as antifungal agent, the activity of Alhagi maurorum was assessed using mycelial radial growth inhibition technique against five plant pathogenic fungi (Rhizocotonia solani , Fusariun moliniforme, Alternaria alternata, Botrytis cinerea and Sclerotia rolfsii). Eethanol extract of Alhagi maurorum displayed a very weak or no antifungal activity against the tested fungi. The antibacterial activity of camelthorn was also assessed against four plant pathogenic bacteria, Agrobacterium tumefaciens, Erwinia amylovolora, Pseudomonas solanacearum and Corynebacterium fascians, using agar dilution method. Hexane extracts of camelthorn showed a remarkable antibacterial activity with minimum inhibitory concentrations (MICs) 3000 mg/l, while ethanol extract exhibited a weak antibacterial activity. GC-MS analysis of ethanolic extract of Alhagi aurorum showed that the major compound have thirteen peaks indicating the presence of thirteen compounds.

 

Author Biography

  • Soad M. Ahmed, Alexandria University, Alex, Egypt
    Fac.,of Agriculture, Pesticides Chemistry dept.

References

Gnanamanickam S.S. Biological Control of Crop Diseases. New York. Basel: Marcel Dekker, Inc., 15 pp., 2002.

Siva, N., Ganesan, S., Banumathy, N. and Muthuchelian, A. Antifungal effect of leaf extracts of some medicinal plants against Fusarium oxysporum causing wilt disease of Solanum melogena L. Ethnobotanical Leaflets. 12: 156-163, 2008.

Amadi, J. E., Salami, S. O. and Eze, C. S. Antifungal properties and phytochemical screening of extracts of African Basil (Ocimum gratissimum L.). Agric Biol. J. North Amer., ISSN Print: 2151-7517, 2010.

Mohana, D. C., Prasad, P., Vijaykumar, V., and Raveesha, K. A. Plant extracts effect on Seed-borne pathogenic fungi from seeds of paddy grown in southern India. Journal of Plant Protectection Research, 51(2): 101-106, 2011.

Rai M. and Carpinella M. Naturally Occurring Bioactive Com¬pounds. Elsevier, Amsterdam, p. 502, 2006.

Paerkh, J. and Chanda, S. In vitro antifungal activity of methanol extracts of some Indian medicinal plants against pathogenic yeast and moulds. African Journal of Biotechnology. 7, 4349-4353, 2008.

Fawzi E.M., Khalil A.A., Afifi A.F. Antifungal effect of some plant extracts on Alternaria alternata and Fusarium oxysporum. Afr. J. Biotechnol. 8 (11): 2590–2597, 2009.

Al-Askar, A. A. and Rashad, Y. M. Efficacy of some plant extracts against Rhizoctonia solani on pea. J. Plant Prot. Res. 50 (3): 239–243, 2010.

Abd-Ellatif, S., Abdel Rahman, S.M. and Deraz, S.F. Promising antifungal effect of some folkloric medicinal plants collected from El- Hammam habitat, Egypt against dangerous pathogenic and toxinogenic fungi. Journal of Agricultural and Biological Science. VOL. 6 (9): 25-32, 2011.

Abdel Rahman, S.M., Abd-Ellatif, S.A., Deraz, S.F. and Khalil, A. A. Antibacterial activity of some wild medicinal plants collected from western Mediterranean coast, Egypt: Natural alternatives for infectious disease treatment. African Journal of Biotechnology Vol. 10(52), pp. 10733-10743, 2011.

Awmack, C.S. and Lock, J. M. The genus Alhagi (Lguminasae: Papilionoideae) in the Middle East. Kew Bull., 57:435-445, 2002.

Hamed, A., Perrone, A., Mahalel, U., Oleszek, W., Stochmal, A. and Piacente. S. Oleanane glycosides from the roots of Alhagi maurorum . Phytochem. Lett., 2012.

Boulose, L. Flora of Egypt Checklist. Al Hadara Publishing, Cairo, Egypt. Pages: 410, 2009.

Hassanein, A.M. and Mazen, M. A. Adventitious bud formation in Alhagi graecorum. Plant Cell Tissue Org. Cult., 65:31-35, 2010.

Marashdah, M.S. and Farraj, A. I. Pharmacological activity of 2% aqueous acetic acid extract of Alhagimaurorum Roots. journal of Saudi Chemical Society( 14): 247–250, 2010.

Awaad, A. S., Maitland, D. J. and Soliman, G. A. Antiulcerogenic Activity of Alhagi maurorum. Pharmaceutical Biology. (44): 292–296, 2006.

Laghari, A., Memon, S., Nelofar, A. and Khan, K. M. Determination of Volatile Constituents and Antimicrobial Activity of Camel Thorn (Alhagi camelorum) Flowers. Anal. Lett., 413 – 421, 2014.

Al-Askar, A. A. In Vitro antifungal activity of three Saudi plant extracts against some phytopathogenic fungi. Journal of Plant Protection Research. 52, (4): 458 – 462, 2012.

Zain, M. E., Awaad, A. S., Al-Outhman, M. R. and El-Meligy, R. M. Antimicrobial activities of Saudi Arabian desert plants. Phytopharmacology. 2(1), 106-113, 2012.

Abu-Taleb, A. M., El-Deeb, K. and Al-Otibi, F. O. Bioactivity of some plant extracts against Drechslera biseptata and Fusarium solani. J. Food Agric. Environ., 9: 769-774, 2011.

Ibrahim, M. T. Anti-inflammatory effect and phenolic isolates of Alhagi graecorum Boiss (Family Fabaceae). Journal of American Science.11, (5), 2015.

Edeoga, H. O., Okwu, D. E. and Mbaebie, B. O. Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology 4, 685–688, 2005.

Kudliki, W. P., William, D., Kramer, S. K., Makhamed, B. G. and Iskakov, B. K. Eukaryotic protein synthesis initiation factor 2. Eur. J. Biochem. 197: 623–629, 1991.

Kalhoro, M. A., Kapadia, Z. and Badar, Y. Physicochemical studies of indigenous medicinal. plants. Bangladesh J. Sci. Ind. Res. 32: 418–421, 1997.

El-Saayed, N. H., Inshak, M. S., Kandil, F. I. and Mabry, T. J. Flavonoids of Alhagi graecorum. Pharmazie. 48: 68–89, 1993.

Singh, V. P., Bineeta, Y. and Pandey, V. B. Flavanone glycosides from Alhagi pseudalhagi. Phytochemistry. 51: 587–590, 1999.

Ahmed, S., Ahmad, I., Saleem, M., Abdul, J., Ur-Rehman, N. And Ul-Hassan, S.S. Secondary metabolites from Alhagi maurorum. J. Chem. Pak. 31, 960–963, 2009.

Behari, M. and Gupt, S.C. The isolation and biogenesis of 24-alkylsterols in Alhagi pseudoalhagi. Acta Cienc. Indica Chem. 6, 207–208, 1980.

Khushbaktova, Z.A., Syrov, V.N., Kuliev, Z., Bashirova, N.S., Shadieva, Z. and Gorodeys-kaia, E.A. The Effect of proanthocyanidins from Alhagi pseudoalhagi Desv on course of experimental myocardial infarct. Eksp. Klin. Farmakol. 55, 19–21, 1992.

Laghari, A.H., Memon, S., Nelofar, A. and Khan, K.M. Alhagi maurorum: a conve-nient source of lupeol. Ind. Crops Prod. 34, 1141–1145, 2011.

Shirwaikar, A., Khan, S., Kamariya, Y. H., Patel, B. D. and Gajera, F. P. Medicinal plants for the management of post-menopausal osteoporosis: a review. Open Bone J., 2: 1-13, 2010.

Turgumbayeva, A. A., Ustenova, G. O., Stabayeva, G. C. and Ross, S. A. Phytochemical Screening and Biological Activities of the Camel Thorn (Alhagi kirghisorum)and Safflower Flowers (Carthamus tinctorius L.) Grown in Kazakhstan. American-Eurasian J. Agric. & Environ. Sci., 14 (12): 1487-1491, 2014.

Awaad, A. S., Mohamed, N. H., Maitland, D. J. and Soliman, G. A. Anti-ulcerogenic Activity of Extract and Some Isolated Flavonoids from .Desmostachia bipinnata (L.) Stapf. Records of Natural Products 3; 76-82, 2008.

Zambonelli, A., Zechini D`Aulerio, A., Bianchi, A. and Albasini, A. Effects of essential oils on phytopathogenic fungi in vitro. J. Phytopathology 144, 491-494, 1996.

Harlapur, S. I., Kulkarni, M. S., Wali, M. C., and Srikantkulkarni, H. Evaluation of plant extracts, bio-agents and fungicides against Exserohilum turcicum causing Turcicum leaf blight of Maize. Journal of Agricultural Science, 20(3):541-544, 2007.

Finney, D. J. Probit analysis. 3rd Ed., (pp. 318). Cambridge University Press, Cambridge, 1971.

European Committee for Antimicrobial Susceptibility Testing (EUCAST) of the European Society of Clinical Microbiology and Infectious Diseases (ESCMID). Determination of minimum inhibitory concentrations (MICs) of antibacterial agents by agar dilution. Clinical Microbiol. and Infection, 6: 509-515, 2000.

Al-Massarani, S. and El Dib, R. In vitro evaluation of cytotoxic and antimicrobial potentials of the Saudi traditional plant Alhagi graecorum boiss. Pak. J. Pharm. Sci., Vol.28, No.3(Suppl), pp.1079-1086, 2015.

Downloads

Published

2015-10-30

How to Cite

In Vitro Activity of Hexane and Ethanol Extracts of Camelthorn, Alhagi maurorum against Plant Pathogenic Fungi and Bacteria. (2015). Asian Journal of Agriculture and Food Sciences, 3(5). https://ajouronline.com/index.php/AJAFS/article/view/3254

Similar Articles

1-10 of 127

You may also start an advanced similarity search for this article.