Morphological and Molecular Systematic of Carangids (Genus: Alepes), with New Record of Alepes vari from the Iraqi Marine Waters,Northwest Arabian Gulf
Keywords:
Morphometric characteristics, DNA fingerprint, PCR-RAPD, Carangids fish, Iraq.Abstract
Morphometric and meristic characteristics and DNA fingerprint analysis were applied to identify three species of Carangidss belonging to the genus Alepes [A. djedaba (Forsskål, 1775), A. kleinii (Bloch, 1793) and A. vari (Cuvier, 1833)] from Iraqi marine waters, during the period from January 2014 to June 2015. A. vari was recorded for the first time in Iraqi waters. Second dorsal fin rays ranged from 22 to 24 in A. djedaba, 25 to 26 in A. kleinii and 26 to 28 in A. vari. Anal fin rays were 19 - 20 in A. djedaba, 21 - 22 in A. kleinii and 20 - 22 in A.vari. Gill rakers on lower limb were 27 - 31 in A. djedaba, 27 – 29 in A. kleinii and 22 – 23 in A. vari. The DNA fingerprints of species of the genus Alepes were identified using Polymerase Chain Reaction-Random Amplified Polymorphic DNA (PCR-RAPD) with six primers: P1 (212), P2 (239), P3 (244), P4 (250), P5 (265), and P6 (347). The numbers of bands generated by primers were 37 in A. djedaba, 51 in A. kleinii and 49 in A. vari. The genetic similarity was 0.38 between A. djedaba and A. kleinii, 0.30 between A. djedaba and A. vari, and 0.24 between A. kleinii and A. vari, while the genetic distance among them were 0.62, 0.70 and 0.76 respectively.
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