The Formation of 8-OHDG from Toxic Substances Trigger to Free Radicals

Authors

  • R. Budiawan Lecturer
  • Intan Cahaya Dani Student
  • Asep Saefumillah Lecturer
  • Sri Handayani Lecturer

Keywords:

Toxicology, 8-OHdG, biomarkers, DNA Adduct

Abstract

This study is conducted to see the profile of DNA Adduct (8-OHdG) formation as DNA damage indicators, by using calf thymus DNA incubated with toxic and carcinogenic compounds. The compounds which could trigger free radicals in this research are PAH(Benzo[a]Pyrene), and CuCl2. Calf thymus DNA was incubated with Benzo[a]Pyrene and CuCl2 compounds under pH and temperature variations to induce the formation of reactive oxygen species (ROS) in the process of oxidative DNA damage. From this research, all of compounds have potency to trigger the formation of DNA Adduct (8-OHdG). The ratio of absorbance to assess the purity of DNA at 260 nm and 280 nm (λ260/ λ280)  is measured at ~1.9. The shifted peaks at λmax are indicating changes on structures of DNA as a result of calf thymus DNA incubation with B[a]P and CuCl2. The highest level of 8-OHdG results in calf thymus DNA incubation with B[a]P and CuCl2 under pH 8.5 and incubation temperature at 60°C, is about 120.856 μg/L.

 

References

• Beach, A.C., Gupta, R.C. 1992. Human Biomonitoring and the 32P-Postlabeling assay, Carcinogenesis 13(7):1053-1074.

• Bryla, P., and Weyand, Eric H. 1991. Role of activated oygen species in Benzo[a]Pyrene : DNA –Adduct Formation in vitro. Free Radical Biology & Medicine, Vol. 11, pp. 17-24, 1991.

• Budiawan and Eder, Erwin. 2000. Detection of 1,N2-Propanodeoxyguanosine Adduct in DNA of Fischer 344 Rats by An Adapted 32P-Post-Labeling Technique After Per Os Application of Crotonaldehyde. Carcinogenesis, vol.21 no.6 pp.1191-1196.

• E. Eder and Budiawan. 2001. Cancer risk assessment for the environmental mutagen and carcinogen crotonaldehyde on the basis of TD50 and comparison with 1,N2-propanodeoxyguanosine adduct levels, Cancer Epidem. Biom. Prev. 10, 883-888

• Guindon, K. 2008. Investigation of The Role of Oxidative DNA Damage in Aflatoxin B1-Induced Pulmonary Carcinogenesis. Departemen Farmakologi dan Toksikologi: Universitas Queen.

• Gunlazuardi.2003.http://www.chem-is-try.org/artikel_kimia/kimia_material/fotokatalis_pada_permukaan_tio2/. Waktu akses 20 Mei 2015

• Hanahan, D., and Weinberg, R.A. 2000. The hallmarks of cancer. Cell 100,57–70.

• Huang, Ning-ping., Xu, Min-hua., Yuan, Chun-wei., Yu, Rui-rong. 1997. The study of the photokilling effect and mechanism of ultrafine TiO2 particles on U937 cells. Department of Biochemistry, Nanjing University, Nanjing, 210093, China Journal of Photochemistry and Photobiology A: Chemistry 108. 229-233.

• Gupta. R. C. Enhanced sensitivity of 12P-postlabeling analysis of aromatic carcinogen: DNA adducts. Cancer Res. 45: 5656-5662. 1985.

• Halliwell, B., M. Whiteman. 2004. Measuring reactive species and oxidative damage in vivo and in cell culture :how should you di it and what do the results mean?. British Journal of Pharmacology, 142, 231-255.

• Hofer, T. 2001. Method Develpoment for Analysis of 8-oxodG as a Biomarker for Oxidative Stress. Departemen Biosains: Institut Karolinska.

• Hiraku, Y. dan Kawanishi, S. 1996. Oxidative DNA Damage and Apoptosis Induced by Benzene Metabolites. Cancer Research, 56, 5172-5178.

• IARC. 1989. Occupational Exposures in Petroleum Refining; Crude Oil and Major Petroluem Fuels. IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Humans, vol. 45. Lyon, France:International Agency for Research on Cancer. 322 pp.

• IARC. 1989. Titanium Dioxide. IARC Summary & Evaluation, Volume 47.

• IPCS WHO (International Programme on Chemical Safet – World Health Organization). 1998. EHC 202 : Selected Non- Heterocyclic Polycyclic Aromatic Hydrocarbons, World Health Organization, Geneva.

• Inoue, O., et al. 2000. Urinary Phenylmercapturic Acid as a Marker of Occupational Exposure to Benzene. Industrial Health, 38, 195-204.

• Kasai, H., Kawai, K. dan Li, Y. 2008. Analysis of 8-OH-dG and 8-OH-Gua as Biomarkers of Oxidative Stress. Genes and Environment. Vol 30, 33-40.

• Kasai H. 1997. Analysis of a form of oxidative DNA damage, 8-hydroxy-2deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis. Mutation Research ;387:147–163.

• Khan, S.A., Carmichael, P.L., Robinson, S.D.Taylor., Habib, N., Thomas H.C. 2003. DNA Adducts, detected by 32P postlabelling, in human cholangiocarcinoma. Gut 2003; 52:586-591.

• Kim EJ, Choi SD, Chang YS. 2011. Levels and patterns of polycyclic aromatic hydrocarbons (PAHs) in soils after forest fires in South Korea. Environ Sci PollutRes Int. Nov;18(9):1508-17.

• Kim, S., dkk. 2006. Using urinary biomarkers to elucidate dose-related patterns of human benzene metabolism. Carcinogenesis, Vol.27, No. 4, 772-781.

• Kim, Sun Young., Taguchi, Takao., Nishioka, Motomu., Taya, Masahito. 2004. Quantitative Assessment of DNA Damage Accompanied with No Substantial Loss in Its Molecular Weight During Exposure to Oxidative Stress. Biochemical Engineering Journal 22 : 81–87.

• Klauning, J.E., Z. Ang et al. 2011. Oxidative Stress and Oxidative Damage in Chemical Carcinogenesis. Toxicology and Applied Pharmacology., 24: 86-99.

• Liu, Z., Lu, Y., R, Barry., L, Mark., Wei, Huachen. 1998. Benzo[a]Pyrene Enhances the Formation of 8-Hydroxy-2’-deoyguanosine by Ultraviolet A Radiation in Calf thymus DNA and Human Epidermoid Carcinoma Cells. Biochemistry, 37, 10307-10312.

• Lodovici, Maura and Bigagli, Elisabetta. 2009. Biomarkkers of Induced Active and Passive Smoking Damage. Int. J. Environ. Res. Public Health, 6 874-888.

• Lorentzen, Ronald J.,Caspary, William J., Lesko, Stephen A. 1975. Autooxidation of 6-hydroxybenzo[a]pyrene and 6-oxobenzo[a]pyrene radical, reactive metabolites of benzo[a]pyrene. Biochemistry, 14(18), pp 3970-3977.

• Minotti, G., Aust, S.D. 1989. The role of iron in oygen radical mediated lipid peroidation. Chem. Biol. 244::6049-6055.

• Mizoue, T., Kasai, H., Kubo, T., dan Tokunaga, S. 2006. Leanness, Smoking, and Enhanced Oxidative DNA Damage. Cancer Epidemiol, Biomarkers Prev., 582-585.

• Phillips, D.H. and Castegnaro, M. 1999. Standardization and Validation of DNA Adduct Postlabelling Methods : Report of Interlaboratory Trials and Production of Recommended Protocols. Mutagenesis, vol.14 no.3 pp.301-315.

• Rachmawati, Widya. 2014. Skripsi : Studi Pembentukan 8-Hidroksi-2’Deoksiguanosin (8-OHdG) dari 2’-Deoksiguanosin dan Guanosin dengan Paparan Senyawa Tert-Butyl Hidroquinone (TBHQ), Depok : Departemen Kimia FMIPA Universitas Indonesia.

• Ramkumae, Kunga Mohan., Manjula, Chinnasamy., Kumar, Georgepeter Gnana., Kanjwal, Muzafar A. 2012. Oxidative stress-mediated cytotoxicity and apoptosis induction by TiO2 nanofibers in HeLa cells. European Journal of Pharmaceutics and Biopharmaceutics 81 (2012) 324–333.

• Teare, J.M., R., Islam., Flanagan, R., Gallagher, S., Davies, M.G., and Grabau, C. 1997. Measurement of Nucleic Acid Concentrations Using the DyNa Quant TM and the GeneQuantTM. Biotechniques 22:1170-1174.

• Tharnpoophasiam, P., Kongtip, P., Wongwit, W., Fungladda, W., dan Kitayaporn, D. 2004. Simultaneous Determination of Trans, Trans-Muconic Acid and S-Phenylmercapturic Acid by High Pressure Liquid Chromatography and Its Application. South East Asian J. Trop. Med. Public Health, Vol. 35, No.3.

• Timbrell, J.A. 1994. Principles of Biochemical Toicology. Taylor & Francis, London

• Valavanidis, A., Vlachogianni, T., dan Fiotakis, C. 2009. 8-hydroxy-2_-deoxyguanosine (8-OHdG): A Critical Biomarker of Oxidative Stress and Carcinogenesis. Journal of Environmental Science and Health Part C, 27:120–139.

• Valko, Marian., Leibfritz, Dieter., Moncol, Jan., Cronin, Mark.T.D., Mazur, Milan., Telser, Joshua. 2007. Free radicals and antioidants in normal physiological functions and human disease. The International Journal of Biochemistry & Cell Biology 39 44-84.

• Vergnoux A, Malleret L, Asia L, Doumenq P, Theraulaz F. 2011. Impact of forest fires on PAH level and distribution in soils. Environ Res, Feb 111(2):193-8.

• Weisburger J.H., and G.M.Williams. 1984. Bioassay of carcinogens : in vitro and in vivo test, in : Chemical Carcinogens, 2nd edition, Vol.2, C.E Searle (ed), ACS Monograph 182, 1323-1373, American Chemical Society, Washington D.C.

• World Health Organization. 2014. News Realeses : Seven million premature deaths annually linked to air pollution. WHO, Geneva.

• Zhang,Li, et al. 2013. Interception of Benzo (a) pyrene- 7,8-dione by UDP Glucuronosyltransferase (UGTs) in Human Lung Cells.

• Zhang, Zhihua and Miriam C. Poirier. 1997. Cisplatin-DNA Adduct Determination in the Hepatic Albumin Gene as Compared to Whole Genomic DNA. 10,971-977

• Zhang, Yu Jing, et al. 1999. Development of Monoclonal Antibody Rcogniing Beno(c)phenanthrenediol Epoxide-DNA Adducts: Application to Immunohistochemical Detection of DNA Damage. 10,948-952

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Published

2016-02-15

How to Cite

Budiawan, R., Dani, I. C., Saefumillah, A., & Handayani, S. (2016). The Formation of 8-OHDG from Toxic Substances Trigger to Free Radicals. Asian Journal of Applied Sciences, 4(1). Retrieved from https://ajouronline.com/index.php/AJAS/article/view/3539