Comparative Study on the Effect of Spices Bioactive Extract on the Stability of Physico-chemical and Sensory Attributes of Zobo Drink under Storage


  • Adedokun I. Ishola Imo State Polytechnic, Umuagwo-Ohaji Imo State Nigeria
  • Stanislaus U. Okorie
  • Blessing C. Nwokeke
  • Ebenezer A. Ike


Zobo-drink, spice extract, Aframomum danielli, black pepper


Effect of local spice extracts on the stability of physico-chemical properties and sensory attributes of Zobo-drink during shelf-life was studied. Varied proportions (4, 8, and 12%) of Aframomum danielli and black pepper extracts were incorporated into Zobo-drink (v/v) during preparation. Physico-chemical properties of samples were determined. Samples obtained from both spice treatments were stored under different conditions (ambient, refrigeration and freezing) for fifteen days. The pH, titrable acidity, brix content and microbial counts were examined at every 72h interval during the storage period. Significant difference (P<0.05) was discovered on the properties of Zobo-drink samples. The mean sensory score differ on the samples attributes in both treatments. The mean scores 8.41 and 8.28 for samples contained 12% and 4% Aframomum and black pepper extracts respectively were the most preferred. The values (%) of 86.49, 1.16, 0.26 and 13.56 highest for moisture, protein, ash and carbohydrates were discovered on samples in both spice treatments. The chemical and microbial properties of stored samples varied significantly. The pH 3.25 and 3.17 (control) for Aframomum danielli and black pepper treated samples changed to pH 1.52 and 1.98 under ambient temperature; pH 3.10 and 3.25 for samples under refrigeration and freezing conditions. The brix content of 3.411 and 3.011 control samples changed to 0.980 and 1.281 at fifteenth day of storage in both spice treatments. Aerobic plate counts (cfumL-1) increased from 1.38 x 103 and 2.10 x103 control samples to 6.61 x103 and 6.28 x103 samples stored at ambient temperature during storage. Coliform bacteria were not detected from the samples during the period of storage.


Author Biography

Adedokun I. Ishola, Imo State Polytechnic, Umuagwo-Ohaji Imo State Nigeria

Department of Food Science and Tchnology




• A.O.A.C (2000). Official methods of analysis. Association of Official Analytical Chemist, 17th edition, Washington D.C.

• Adegoke G.O. and Skura, B.J (1994). Nutritional profile and antimicrobial spectrum of the spice Aframomum danielli K. Schum. Plant Food Human Nutri. 45: 175-182.

• Adegoke, G. O., Iwahashi, H., Komatsu, Y., Obuchi, K. and Iwahashi Y. (2000) inhibition of food spoilage yeasts and afflatoxigenic moulds by monoterpenes of the spice Aframomum danielli: Flavor and Fragrance. Journal 15 (3): 147-150.

• Adegoke, G. O., Krishna, A. G. G. and Gopala Krishna A. G, (1998) Extraction and Identification of Antioxidants from the Spice Aframomum danielli: Journal of the American Oil Chemists’ Society 75(8), 1047-1052.

• Ajibola A. D., Beatrice O. T. I., Fashakin J F (2013). Antimicrobial and Antioxidant Properties of Selected Local Spices Used In “Kunun†Beverage In Nigeria Acta Sci. Pol., Technol. Aliment. 12(4): 373-378.

• Akinyosoye F.A. and Akinyele, B.J. (2007). Microorganisms associated with ‘zoborodo’ a Nigeria beverage. Niger Soc. Microbial Book Abstract, pp 23.

• Akpomodaye, D. E. and Ejechi, B.O. (1998). The handling effect of mild heat and two tropical spices extract on growth of three fungi in fruit. Food Res. International 31(50):33-341.

• Amoo I.A. (2006). Characterization of the extract of calyces of roselle as a source of “sobo†drink in Nigeria. Natural P h y t o c h e m i s t r y (1): 56-58

• Bolade M.K., Oluwalana I.B. and Ojo O. (2009): Commercial Practice of Roselle (Hibiscus sabdariffa L.) Beverage Production: Optimization of Hot Water Extraction and Sweetness level, World Journal of Agricultural Sciences 5 (1): 126-131.

• Cheesbrough, M. (2000). District Laboratory Practices in Tropical Countries, Part 2, Cambridge University Press, UK. Pp35-38, 62-69.

• Dnyaneshwar J., Mayur L., Amol G., Vaishali B. (2013). Study on Effect of Carbonation on Storage and Stability of Pineapple Fruit Juice. International Journal of Engineering Research & Technology, 2 (12): 332-339

• Elgayyar, M., Draughon, F. A. Golden, D. A. Mount. J. R. (2001). Antimicrobial activity of essential oils from plants against selected pathogenic and saprophytic microorganisms, Journal of Food Protection, Vol.64, No.7, 1091-1024.

• Emmanuel S. A., Taiwo O. O. and Grace O. (2006). Studies on the storage stability of soursop (Annona muricata L.) juice. African Journal of Biotechnology 5 (19), Pp. 1808-1812

• FAO (2000). Food and Agriculture Organization of the United Nations on Food composition table for use in Africa- West Tsuen WU Leuing Felix Busson and Clande Jardin (Eds) Rome, Italy.

• Fasoyiro, S.B, Babalola, S.O. and Owosibo, T. (2005). Chemical Composition and Sensory Quality of Fruit-Flavoured Roselle (Hibiscus sabdariffa) Drinks. World Journal of Agricultural Sciences 1 (2): 161-164.

• Fasoyiro, S.B., Adegoke, G. O., Ashaye, O. A., Obatolu, V. A. and Owolade, O. F. (2001) Antimicrobial Characteristics of Aframomum danielli spice on Esherichia coli and Staphylococcus aureus. Moor Journal of Agricultural Research, 2 (2): 159-161.

• ICMSF (1997): International commission on microbiological specifications for foods. Microorganisms in foods. Microbiological specifications of food pathogens. Clays Ltd, St Ives Plc, Bungay Suffolk, UK.

• Igual M., García-Martínez, E., Camacho M.M., Martínez-Navarrete N. (2010) Effect of thermal treatment and storage on the stability of organic acids and the functional value of grapefruit juice. Food Chemistry 118 (291–299

• Martins, A. P., Salgueiro, L., Goncalves, M. J., Cunha, A. P. da, Vila, R., Canigueral, S., Mazoni, V., Tomi, F., Casanova, J. and da Cunha, A. P. (2001) Essential Oil Composition and Antimicrobial Activities of Three Zingiberaceae from Sao Tome and Principe: Planta Medica 67 (6) 580-584.

• Mazza, G. and Brouillard, R. (1990). The mechanism of co-pigmentation of anthocyanins in aqueous solutions. Phyto-chemistry, 29: 1097-1102.

• Michael H. Gordon (2001) The development of oxidative rancidity in food In: Antioxidants in food, practical application. 1st edition,Wood head Publishing Ltd. And CRC Press LLC, England. Pp 17.

• Ogiehor M.T., Owhe-Ureghe U.B and Momodu, I.O (1998). Microbial, chemical and organoleptic quality of palm wine preserved by combination of preservatives. Nigeria Journal of Microbial 12(1-2): 63-68.

• Ogiehor, I.S and Nafor EO.E (2004). Associated Microbiological Biochemical and chemical qualities changes in zobo beverages produced from Hibiscus sabdarrifa Linn. Niger Ann National Science 5(2):-10.

• Oluwalana, I.B, Ade-Omowaye, B.I.O and Adedeji, T.O (2013): Proximate and Mineral composition of A sorghum stem sheath beverage (Poporo) spiced with Aframomum melegueta extract. British Journal of Applied Science and Technology 3(4):973-983

• Oluwaniyi OO, Dosumu OO, Awolola GV, Abdulraheem AF. . (2009) Nutritional analysis and stability studies of some natural and synthetic food colourants. American Journal of Food Technology;4(5):218-225.

• Pearson, D (1976). The chemical analysis of food. 7th edition. Church-Hill Livingstone, New York, USA. Pp572.

• Pelezar, M.J, (Jr.)., Chan, E.C.S., and Krieg, N.R. (1993). Microbilogy Concepts and Applications, 1st ed. McGraw-Hill Inc. New York, USA. pp. 80-100, 158-161, 370.

• Reddy, V., Urooj, A., Kumar. A. (2005). Evaluation of antioxidant activity of some plant extracts and their application in biscuits, Food Chemistry, Vol.90, 317-321.




How to Cite

Ishola, A. I., Okorie, S. U., Nwokeke, B. C., & Ike, E. A. (2015). Comparative Study on the Effect of Spices Bioactive Extract on the Stability of Physico-chemical and Sensory Attributes of Zobo Drink under Storage. Asian Journal of Agriculture and Food Sciences, 3(2). Retrieved from