Occurrence of Alfalfa Mosaic Virus (AMV) Infecting Bean Crop in Burdur Province, Turkey
Keywords:
Virus, detection, ELISA, IC-RT-PCRAbstract
During surveys conducted in 2012- 2013 growing season, field-grown bean plants with symptoms of mosaic, chlorotic mottling, vein banding, blistering and leaf malformation were observed. A total of 118 leaf samples were collected from various locations in this region. Alfalfa mosaic virus (AMV), was detected in bean, using enzyme-linked immunosorbent assay (ELISA), and immunocapture-reverse-transcription-polymerase chain reaction (IC-RT-PCR) methods. ELISA tests showed that among 118 field samples tested, 7 were infected with AMV. Seven leaf samples that had tested positive in DAS-ELISA were used for IC-RT-PCR. IC-RT-PCR was carried out by using specific primer which amplified a 351 bp fragment of coat protein of AMV in samples. The presence of AMV in the leaf samples was further confirmed IC-RT-PCR using specific primers.
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