Synergistic Effect of Permeable and Impermeable Cryoprotectants on the Survival and Hatching Rate of Sutchi Catfish (Pangasius hypophthalmus) Embryos Treated at Subzero Temperatures

Authors

  • Bui Xuan Nguyen Vietnamese Academy of Science and Technology
  • Nguyen Thi Hong Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi,
  • Nguyen Thi Uoc Lab. of Embryo Biotechnology, Institute of Biotechnology, Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi,
  • Nguyen Thi Nhung
  • Nguyen Thi Hiep
  • Nguyen Trung Thanh
  • Ha Thi Ngoc Nga
  • Truong Van Thuong Bac Ninh fisheries colleges, Dinh Bang, Tu Son, Bac Ninh, Vietnam
  • Nguyen Viet Linh Lab. of Embryo Biotechnology, Institute of Biotechnology, Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi,
  • Nguyen Van Hanh Lab. of Embryo Biotechnology, Institute of Biotechnology, Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi,
  • Beck-Woerner Barbara Lab. of Embryo Biotechnology, Institute of Biotechnology, Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi,

Keywords:

Catfish, Cryoprotectants, Embryos, low temperature, Hatching rate

Abstract

ABSTRACT- The sutchi catï¬sh (Pangasius hypophthalmus) has been widely introduced for aquaculture industry in Asiatic countries. The cryopreservation of catfish embryos is important to facilitate stock enhancement programs.The present study was designed to determine the effect of combination of permeable and impermeablecryoprotectants on survival and hatching of catfish embryos aiming to understand the basic conditions for their conservation by vitrification. Sutchi catfish embryos at somites and optic cups were treated at room temperature, 4 oC, 0 oC and -20 oC, with impermeable sucrose as a factor of dehydration and membrane stability, separately or in different combination with glycerol, methanol (MeOH), ethylene glycol (EG), and propanediol (PG). The obtained results show that the catfish embryos are sensitive to low temperature and the hatching rate was significantly decreased for embryos treated at temperature below 0 oC. Catfish embryos are able to perform a partial dehydration in 0.5 M, 1.0 M and 2.0 M sucrose, respectively. Dehydration with 2.0 M sucrose has high protective effect for embryos treated at 0 oC. The combination of dehydration and MeOH, PG and EG at high concentration of more than 5.0 M can be used safely to keep embryos at 0 oC and -20 oC for short duration. In conclusion, present information related to cryobehavior and cryoprotectants was established for further study of vitrification in catfish embryos.

Author Biographies

Bui Xuan Nguyen, Vietnamese Academy of Science and Technology

Lab. of Embryo Biotechnology, Institute of Biotechnology, Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi,

Nguyen Thi Hong, Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi,

Lab. of Embryo Biotechnology, Institute of Biotechnology, Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi,

Nguyen Thi Nhung

Lab. of Embryo Biotechnology, Institute of Biotechnology, Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi,

Nguyen Thi Hiep

Lab. of Embryo Biotechnology, Institute of Biotechnology, Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi,

Nguyen Trung Thanh

Lab. of Embryo Biotechnology, Institute of Biotechnology, Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi,

Ha Thi Ngoc Nga

Department of Environment and Pathology, Research Institute for Aquaculture No.2, Cai Be, Tien Giang, Vietnam 

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Published

2014-06-14

How to Cite

Nguyen, B. X., Hong, N. T., Uoc, N. T., Nhung, N. T., Hiep, N. T., Thanh, N. T., Ngoc Nga, H. T., Thuong, T. V., Linh, N. V., Hanh, N. V., & Barbara, B.-W. (2014). Synergistic Effect of Permeable and Impermeable Cryoprotectants on the Survival and Hatching Rate of Sutchi Catfish (Pangasius hypophthalmus) Embryos Treated at Subzero Temperatures. Asian Journal of Agriculture and Food Sciences, 2(3). Retrieved from https://ajouronline.com/index.php/AJAFS/article/view/1343

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