Dual Lipases System in Transesterification of Ethyl Ferulate with Olive Oil: Optimization by Response Surface Methodology

Authors

  • Nurul Jannah Abd Rahman Universiti Sains Islam Malaysia
  • Salina Mat Radzi
  • Hanina Mohd Noor

Keywords:

Dual lipases system, transesterification, ferulate esters, optimization

Abstract

A novel and simple dual lipases system was effectively developed in attaining high performance of ferulate esters by transesterification between ethyl ferulate and olive oil. Enzyme screening revealed 1: 9 w/w of Novozym 435-Lipozyme RMIM to be the most efficient lipases ratio for the reaction synthesis. Response surface methodology (RSM) based on four-factor-five-level central composite design (CCD) was used to study interactive effects of reaction factors: time, lipase dosage, ratio substrates and temperature. A high percent conversion of 94.03 % was achieved under the optimum conditions of 12 h, 87 mg lipases, 1: 2 ratio substrates and 60 áµ’C, which compared well with the maximum predicted value of 94.83 %.

References

Palla CA, Carrin ME, “Kinetics Modelling of The Acidolysis with Immobilized Rhizomucoe Miehei Lipases for Production of Structured Lipids from Sunflower Oilâ€, Biochemical Engineering Journal, vol. 90, pp. 184-194, 2014.

Ciftci D, Saldana MDA, “Enzymatic Synthesis of Phenolic Lipids Using Flaxseed Oil and Ferulic Acid in Supercritical Carbon Dioxide Mediaâ€, Journal of Supercritical Fluids, vol. 72, pp. 255-262, 2012.

Ma N, Hong SI, Zhao TT, No DS, Kim CT, Kim Y, Kim IH, “Enrichment of Docosahexaenoic Acid from Tuna Oil via Lipase-Mediated Esterification under Pressurized Carbon Dioxideâ€, Journal of Supercritical Fluids, vol. 87, pp. 28-33, 2014.

Barone E, Calabrese V, Mancuso C, “Ferulic Acid and Its Therapeutic Potential as a Hormetin for Age-Related Diseasesâ€, Biogerontology, vol. 10, pp. 97-108, 2009.

Sun S, Zhu S, Bi Y, “Solvent-Free Enzymatic Synthesis of Feruloylated Structured Lipids by the Transesterification of Ethyl Ferulate with Castor Oilâ€, Food Chemistry, vol. 158, pp. 292-295, 2014.

Sun S, Song F, Bi Y, Yang G, Liu W, “Solvent-Free Enzymatic Transesterification of Ethyl Ferulate and Monostearin: Optimized by Response Surface Methodologyâ€, Journal of Biotechnology, vol. 164, no. 2, pp. 340-345, 2013.

Yang Z, Glasius M, Xu X, “Enzymatic Transesterification of Ethyl Ferulate with Fish Oil and Reaction Optimization by Response Surface Methodologyâ€, Food Technology and Biotechnology, vol. 50, no. 1, pp. 88-97, 2012.

Compton DL, Laszlo JA, Berhow MA, “Lipase-Catalyzed Synthesis of Ferulate Estersâ€, Journal of the American Oil Chemists' Society, vol. 77, no. 5, pp. 513-519, 2000.

Lin FH, Lin JY, Gupta RD, Tournas JA, Burch JA, Selim MA, Monteiro-Riviere NA, Grichnik JM, Zielinski J, Pinnell SR, “Ferulic Acid Stabilizes a Solution of Vitamins C and E and Doubles Its Photoprotection of Skinâ€, Journal of Investigative Dermatology, vol. 125, pp. 826-832, 2005.

Murakami A, Nakamura Y, Koshimizu K, Takahashi D, Matsumoto K, Hagihara K, Taniguchi H, Nomura E, Hosoda A, Tsuno T, Maruta Y, Kim HW, Kawabata K, Ohigashi H, “FA15, A Hydrophobic Derivative of Ferulic Acid, Suppresses Inflammotary Responses and Skin Tumor Promotion: Comparison with Ferulic Acidâ€, Journal of Cancer Letters, vol. 180, pp. 121-129, 2002.

Xin JY, Chen LL, Zhang YX, Zhang E, Xia CG, “Lipase Catalyzed Transesterification of Ethyl Ferulate with Triolein in Solvent-Free Mediumâ€, Food and Bioproducts Processing, vol. 89, pp. 457-462, 2011.

Chattopadhyay S, Sen R, “Development of a Novel Integrated Continuous Reactor System for Biocatalytic Production of Biodieselâ€, Bioresourse Technology, vol. 147, pp. 395-400, 2013.

Reyes-Duarte D, Lopez-Cortes N, Torres P, Comelles F, Parra JL, Peña S, Ugidos AV, Ballesteros A, Plou FJ, “Synthesis and Properties of Ascorbyl Esters Catalyzed by Lipozyme TL IM Using Triglycerides as Acyl Donorsâ€, Journal of American Oil Chemists’ Society, vol. 88, pp. 57-64, 2011.

Fjerbaek L, Christensen KV, Norddahl B, “A Review of the Current State of Biodiesel Production Using Enzymatic Transesterificationâ€, Biotechnology and Bioengineering, vol. 102, no. 5, pp. 1298–1315, 2009.

Malhotra D, Mukherjee J, Gupta MN, “Lipase Catalysed Tranesterification of Castor Oil by Straight Chain Higher Alcoholsâ€, Journal of Bioscience and Bioengineering, vol. x, pp. 1-4, 2014.

Banerjee A, Singh V, Solanki K, Mukherjee J, Gupta MN, “Combi-Protein Coated Microcrystals of Lipases for Production of Biodiesel from Oil From Spent Coffee Groundsâ€, Sustainable Chemical Processes, vol. 1, no.1, pp. 14, 2013.

Lee JH, Kim SB, Kang SW, Song YS, Park C, Ok S, Seung H, Kim W, “Biodiesel Production by A Mixture of Candida Rugosa and Rhizopus Oryzae Lipases Uing A Supercritical Carbon Dioxide Processâ€, Bioresource Technology, vol. 102, pp. 2105-2108, 2011.

Rodrigues RC, Ayub MAZ, “Effects of the Combined Use of Thermomyces Lanuginosus and Rhizomucor Miehei Lipases for the Transeterification and Hydrolysis of Soybean Oilâ€, Process Biochemistry, vol. 46, pp. 682-688, 2011.

Guan F, Peng P, Wang G, Yin T, Peng Q, Huang J, Guan G, Li Y, “Combination of Two Lipases More Efficiently Catalyzes Methanolysis of Soybean Oil for Biodiesel Production in Aqueous Mediumâ€, Process Biochemistry, vol. 45, no. 10, pp. 1677-1682, 2010.

Ibrahim NA, Guo Z, Xu X, “Enzymatic Interesterification of Palm Stearin and Coconut Oil by a Dual Lipase Systemâ€, Journal of American Oil Chemists’ Society, vol. 85, pp. 37-45, 2008.

Kuo CH, Chen HH, Chen JH, Liu YC, Shieh CJ, “High Yield of Wax Ester Synthesized from Cetyl Alcohol and Octanoic Acid by Lipozyme RM IM and Novozym 435â€, International Journal of Molecular Sciences, vol. 13, pp. 11694-11704, 2012.

Roy I, Gupta MN, “Hydrolysis of Starch by a Mixture of Glucoamylase and Pullulanase Entrapped Individually in Calcium Alginate Beadsâ€, Enzyme and Microbial Technology, vol. 34, no. 1, pp. 26-32, 2004.

Syamsul KMW, Salina MR, Siti SO, Hanina MN, Basyaruddin MAR, Kamaruzaman Jusoff, “Green Synthesis of Lauryl Palmitate via Lipase-Catalyzed Reactionâ€, World Applied Sciences Journal, vol. 11, no 4, pp. 401-407, 2014.

Salis A, Solinas V, Monduzzi M, “Wax Esters Synthesis from Heavy Fraction of Sheep Milk Fat and Acetyl Alcohol by Immobilized Lipasesâ€, Journal of Molecular Catalysis B: Enzymatic, vol. 21, no. 4-6, pp.167-174, 2003.

Oguntimein GB, Anderson WA, Moo-Young M, “Synthesis of Geraniol Esters in a Solvent-Free System Catalysed by Candida Antarctica Lipaseâ€, Biotechnology Letters, vol. 17, pp. 77-82, 1995.

Stamatis H, Sereti V, Kolisis FN, “Enzymatic Synthesis of Hydrophobic Derivatives of Natural Phenolic Acids in Organic Mediaâ€, Journal of Molecular Catalysis B: Enzymatic, vol. 11, no. 4-6, pp. 323-328, 2001.

Beg Q, Sahai V, Gupta R, “Statistical Media Optimization and Alkaline Protease Production from Bacillus Mojavensis in Bioreactorâ€, Process Biochemistry, vol. 39, no. 2, pp. 203-209, 2003.

Dixon M, Webb E, Enzymes, Longman, London, 1979.

Carvalho CML, Serralheiro MLM, Cabral JMS, Aires-Barros MR, “Applications of Factorial Design to the Study of Transesterification Reactions using Cutinase in AOT-Reversed Micellesâ€, Enzyme and Microbial Technology, vol. 21, pp. 117-123, 1997.

Xin JY, Zhang L, Chen LL, Zheng Y, Wu XM, Xia CG, “Lipase-Catalyzed Synthesis of Ferulyl Oleins in Solvent-Free Mediumâ€, Food Chemistry, vol. 112, pp. 640-645, 2009.

Downloads

Published

2015-03-04

How to Cite

Abd Rahman, N. J., Radzi, S. M., & Noor, H. M. (2015). Dual Lipases System in Transesterification of Ethyl Ferulate with Olive Oil: Optimization by Response Surface Methodology. Asian Journal of Applied Sciences, 3(1). Retrieved from https://ajouronline.com/index.php/AJAS/article/view/2112

Issue

Vol. 3 No. 1 (2015): February 2015

Section

Articles

License

  • Papers must be submitted on the understanding that they have not been published elsewhere (except in the form of an abstract or as part of a published lecture, review, or thesis) and are not currently under consideration by another journal published by any other publisher.
  • It is also the authors responsibility to ensure that the articles emanating from a particular source are submitted with the necessary approval.
  • The authors warrant that the paper is original and that he/she is the author of the paper, except for material that is clearly identified as to its original source, with permission notices from the copyright owners where required.
  • The authors ensure that all the references carefully and they are accurate in the text as well as in the list of references (and vice versa).
  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Attribution-NonCommercial 4.0 International that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
  • The journal/publisher is not responsible for subsequent uses of the work. It is the author's responsibility to bring an infringement action if so desired by the author.