Evaluation of the Toxicity Effect of Tephrosia purpurea Extracts against Filarial Vector Culex quinquefasciatus
Keywords:
Methanolic extract, Toxicity, Fecundity, Tephrosia purpurea, Culex quinquefasciatusAbstract
Excessive in using insecticides has led to damage to the environment and public health, which led to reconsideration in use the natural pesticides of plant origin in the fight against mosquitoes. Laboratory studies were conducted to evaluate the toxicity effect of methanolic leaves and stems extracts  of the plant Tephrosia purpurea (Fabaceae) against the mosquito Culex quinquefasciatus. The efficacy of stems extract against 3rd instar larvae seemed to be less effective with Lc50 2348ppm than leaves extract with Lc50 58.3ppm. Leaves extract showed a remarkable reduction effects on adult emergence, fecundity and fertility more than stems extract. Both leaves and stems extract exerted delayed toxic effect on the pupae and adults resulted from treated larvae. Furthermore, some morphological deformities (pupal-adult intermediate) were observed after treatment with leaves extract. The current results considered promising to move forward in studying the bioactive plants which form an environmentally sound alternative for the synthetic insecticides.
References
• Abahussain, M.O. (1999): Effect of Sorghum bicolor and Nerium oleander extracts on of the grey flesh fly Parasarcophaga argyrostoma (Diptera : sarcophagidae). J. Egypt. Ger. Soc. Zool., 28(2): 233 – 243.
• Bansal, S.K.; Karam, V. S.; Sharma, S. and Sherwani, M.R.K. (2012): Laboratory observations on the larvicidal efficacy of three plant species against mosquito vectors of malaria, Dengue/Dengue Hemorrhagic Fever (DF/DHF) and lymphatic filariasis in the semi-arid desert. J. Environ. Biol., 33: 617-621
• Briggs, J.N.(1960): Reduction of adult house fly emergence by the effective Bacillus sp. on the development of immature forms. J. Insect pathology. 2 : 418 – 432.
• Chalannavar, R.K., Hurinanthan, V., Singh, A., Venugopala, K.N., Gleiser, R.M., Baijnath, H. and Odhav, B.(2013): The antimosquito properties of extracts from flowering plants in South Africa. Tropical Biomedicine 30(4): 559–569.
• Corbel, V., Duchon, S., Zaim, M., Hougard, J.M., (2004): Dinotefuran: a potential neonicotinoid insecticide against resistant mosquitoes. J. Med. Entomol. 41 (4), 712–717.
• El-Sheikh, T.M.Y.; Hassan, M.I.; Moselhy, W.A.; Amer, M.S. and Shehata, A.Z. (2011): Evaluation of the biological activity of some Cupressus semprevirens (Cupressaceae) extracts against the mosquito vector Culex pipiens L. (Diptera: Culicidae). Egypt. Acad. J. biolog. Sci., 4 (1): 33 – 48.
• Finney, D.J. (1971): Probit analysis Third edition. Cambridge Univ. Press., 333 p.
• Gad, A.M.; Hammad, R.E. and Farid, H.A. (1996): Uptake and development of Wucheria bancrofti in C. pipiens L. and Ae. caspius Pallas. J. Egypt. Soc. Parasitol., 26(2): 305 – 314.
• Goddard, L.B., Roth, A.E., Reisen,W.K., Scott, T.W., (2002): Vector kompetence of Californiam mosquitoes for West Nile virus. Emerg. Infect. Dis. 8, 1385–1391.
• Govindarajan, M. and Rajeswary,M.(2014): Mosquito larvicidal properties of Impatiens balsamina (Balsaminaceae) against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae). Journal of Coastal Life Medicine 2014; 2 (3): 222-224.
• Harbach, R.E.,(1985): Pictorial keys to the genera of mosquitoes, subgenera of Culex and the species of Culex (Culex) occurring in southwestern Asia and Egypt, with a note on the subgeneric placement of Culex deserticola (Diptera: Culicidae). Mosq. Syst. 17: 83-l 07.
• Ilahi I. and UllahF.(2013): Larvicidal activities of different parts of Artemisia vulgaris Linn. against Culex quinquefasciatus Say. (Diptera: Culicidae). International Journal of Innovation and Applied Studies, 2 (2), 189 – 195.
• Jeyabalan, D.; Arul, N. and Thangamathi, P. (2003): Studies on effects of Pelargonium citrosa leaf extracts on malarial vector, A. stephensi Liston. Bioresour. Technol., 89(2): 185 – 9.
• Kasap, M. and Demirhan (1992): The effect of various larval foods on the rate of adult emergence and fecundity of mosquitoes. Turkiye Parazitologi Dergisi., 161 : 87 – 97.
• Kolli G. R., Balakrishnan, Vijayan, and SundararajanR. (2013): Evaluation of larvicidal activity of Pongamia pinnata extracts against three mosquito vectors. Asian Pac. J. Trop. Biomed. 3(11): 853-858.
• Kovendan, K.; Murugan, K.; Vincent, S. and Barnard, D.R. (2012): Mosquito larvicidal properties of Orthosiphon thymiflorus (Roth) Sleesen. (Family: Labiatae) against mosquito vectors, Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti (Diptera: Culicidae). Asian Pac. J. Trop. Med. 5(4): 299 – 305.
• Kumar, D.; Dhamodaran, P.; Nilani P. and Balakrishnan N. (2012): Larvicidal Activity of Tephrosia purpurea, (L) Against the Larvae of Culex quinquefasiciatus. J. Applied Pharmaceutical Science 2 (7) : 219-221
• Lentner, C.; Lentner, C. and Wink, A. (1982): Studentis t- distribution tables. In Geigy scientific Tables Vol. 2. International Medical and Pharmaceutical information, Ciba – Geigy Limited, Basal, Switzerland.
• Maurya, P.; Sharma, p.; Mohan, L. ; Batabyal L. and Srivastava, C.N. (2009): Evaluation of the toxicity of different phytoextracts of Ocimum basilicum against Anopheles stephensi and Culex quinquefasciatus. Journal of Asia-Pacific Entomology 12 : 113–115.
• Migahid, A. M. (1987): Flora of Saudi Arabia. Fourth Edition, King Saud University, Riyadh.
• Mohan, D.R., Ramaswamy, M., (2007): Evaluation of the larvicidal activity of the leaf extract of a weed plant, Ageratina adenophora, against two important species of mosquitoes, Aedes aegypti and Culex quinquefasciatus. Afr. J. Biol. 6, 631–638.
• Nathan, S.S.; Savitha, G.; George, D.K.; Narmadha, A.; Suganya, L. and Chung, P.G. (2006): Efficacy of Melia azedarach L. extract on the malarial vector A. stephensi Liston (Diptera: Culicidae). Bioresour Technol. 79: 1316 – 1323.
• Patil, C.D.; Patil, S.Y.; Salunke, B.K. and Salunkhe, R.B. (2011): Bioefficacy of Plumbago zeylanica (Plumbaginaceae) and Cestrum nocturnum (Solanaceae) plant extracts against Aedes aegypti (Diptera: Culicide) and nontarget fish Poecilia reticulata. Parasitol. Res. 108(5): 1253 – 63.
• Pavela, R., (2009): Larvicidal property of essential oils against Culex quinquefasciatus Say (Diptera: Culicidae). Ind. Crops Prod. 30, 311–315.
• Raveen, R., Kamakshi, K.T., Deepa, M., Arivoli S. and Samuel Tennyson (2014): Larvicidal activity of Nerium oleander L (Apocynaceae) flower extracts against Culex quinquefasciatus Say (Diptera: Culicidae). International Journal of Mosquito Research, 1 (1): 38-42.
• Rohani, A., Chu,W.L., Saadiyah, I., Lee, H.L., Phang, S.M., (2001): Insecticide resistance status of Aedes albopictus and Aedes aegypti collected from urban and rural in major towns of Malaysia. Trop. Biomed. 18 (1), 29–39.
• Roberts, L., (2002): Mosquitoes and disease. Science 298, 82–83. Shalaby, A.A., Allam, K.A.M., Mostafa, A.A., Fahmy, S.M.E., 1998. Insecticidal properties of citrus oils against C. pipiens and M. domestica. J. Egypt. Soc. Parasitol. 28 (2), 595–606.
• Sakthivadivel M.; Eapen A., and Dash A.P.(2012): Evaluation of toxicity of plant extracts against vector of lymphatic filariasis, Culex quinquefasciatus. Indian J. Med. Res. 135:397-400.
• Sharma, p.; Mohan, L. and Srivastava, C.N. (2006): Growth inhibitory nature of Artemisia annua extract against Culex autnauetesctetus (Say). J. Asia-Pacific Entomol. 9(4): 389-395.
• Sukumar, K.; Perich, M.J. and Boobar, L.R. (1991): Botanical derivatives in mosquito control: A review. Am. Mosq. Control. Assoc., 7(2): 210 – 237.
• Touqeer, S., Saeed M.A. and Ajaib, M.(2013): A Review on the Phytochemistry and Pharmacology of Genus Tephrosia. Phytopharmacology. 4(3), 598-637.
• WHO, (1985): Mineographed document.
• Williams, K.A.; Green, D.W.J.; Pascoe, D. and Gower, D.E. (1986): The acute toxicity of cadmium to different larval stages of Chrionomus riparius (Diptera : Chironomidae) and its ecological significance for pollution regulation. J. Sci. Unvi. Tokushima. 22, 25–31.
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