The Effect of Betel Quid Extract on Wound Healing Process in Male Wistar Rats (Rattus norvegicus L.)

Authors

  • Siti Rusdiana Puspa Dewi Universitas Sriwijaya
  • Adelina Fatonah Universitas Sriwijaya

DOI:

https://doi.org/10.24203/ajas.v7i6.6035

Keywords:

anti-inflammatory, betel quid, ephitelial thickness, wound healing

Abstract

Wound can be occured during dental treatment. In outline, there are several phases of wound healing; inflammatory phase, proliferative phase, and maturation phase. Various drugs in various forms are used to accelerate the healing process, but mostly they have side effects. Therefore, traditional medicine, such as betel quid consisted of betel leaf, areca nut, gambier, and calcium hydroxide, is developed. The aim of this study was to investigate the effect of wound healing process in male Wistar rats. A total of 30 male Wistar rats were taken and divided into 5 groups:  Group 1, 2, and 3 (K1,K2, K3)  were given 5%, 10% and 15% concentrations of betel quid extract oinments; Group 4 (K4) was positive control  (hyaluronic acid 0.2% oinment); Group 5 (K5) was negative control (placebo oinment). One mm- diameter of lower lip mucosal wounds on rats were created by using  cylinder diamond bur. The oinments were applied twice daily for 10 days. The number of netrophils on first and third day were measured and the thickness of ephitelium on 10 days were determined. All groups of  betel quid extracts exhibited the reduction of the number of neutrophils on inflammatory phase. Group 3 shown as the highest effect and had no significant different with positive control. Betel quid extracts in all groups also improved epithelial thickness on proliferative phase, in which group II and group III had no significant different with positive control. Betel quid extract had effect on wound healing process in male Wistar rats due to its ability in supressing inflamation and in increasing reepithelization.

Author Biography

Siti Rusdiana Puspa Dewi, Universitas Sriwijaya

Departement of Biomedical Science

References

S. Dhivya, V. V. Padma, E. Santhini, “Wound dressings- a review,†Biomedicine (Taipei), vol. 5, no. 4, pp 22-29, 2015.

M. Sharma, B. S. Khajja, S. Jha, G.K Mathur, and V. N. Mathur, “Forensic interpretation of injuries/wounds found in the human body,†Journal of Punjab Academic Forensic Medicine and Toxicology, vol. 11, no. 2, pp 105-109, 2011

N. Ghanapathy, S. S. Venkataraman, R. Daniel, R. J. Aravind, and V. B. Kumarakhrisnan, “Molecular biology of wound healing,†Journal of Pharmacy and BioAllied Sciences, vol. 2, no. 2, pp 334-337, 2012.

H. Sinno and S. Prakash, “Complements and the wound healing cascade: an update review,†Plastic Surgery International, vol. 2013, pp 1-7, 2013.

K. Parthiban, S. Manikandan, and S. Ganesapandian, “Production of cellulose I microfibrils from Rhizobium sp. and its wound healing activity on mice,†Asian Journal of Applied Sciences, vol 4, pp. 247-254, 2011.

A. C. D. O. Gonzales, T. F. Costa, Z. A. Andrade, and A. R. A. P. Medrado, “Wound healing- a literature review,†Anais Brasileiros de Dermatologia, vol. 91, no. 5, pp. 614-620, 2016.

A. J. Gale, Current understanding of hemostasis,†Toxicologic Pathology, vol. 39, no. 1, pp 273-280, 2011.

K. Ghoshal, and M. Bhattacharyya, “Overview of platelet physiology: its hemostatik role in disease pathogenesis,†The Scientific World Journal, vol. 2014, pp. 1-16, 2014.

S. Guo, and L. A. DiPietro, “Factors affecting wound healing,†Journal of Dental Research, vol. 89, no. 3, pp. 219-229, 2010

Flanagan M, “The physiology of wound healing,†Journal of Wound Care, vol. 9, no. 6, pp. 299-300, 2000

E. Kolaczkowska, “The older the faster: aged neutrophils in inflammation,†Blood, vol. 128, pp. 2280-2282, 2016.

D. J. Lee, H. Li, M. T. Ochoa, M. Tanaka, et al, “Integrated pathways for neutrophil recruitment and inflammation in Leprosy,†The Journal of Infectious Disease, vol.201, no. 4, pp. 558-569, 2010

J. Li, J. Chen, and R. Kirsner, “Patophysiology of acute wound healing,†Clinical Dermatology, vol. 25, no. 1, pp. 9-18, 2007

A. Bhatia, K. O’Brien, M. Chen, D. T. Woodley, and W. Li, “Keratinocyte-secreted heat shock protein-90 alpha: leading wound reepithelization and closure,†Advance on Wound Care, vol. 5, no. 4, pp. 176-184, 2016

K. E. Johnson and T. A. Wilgus, “Vascular endothelial growth factor and angiogenesis in the regulation of cutaneous wound repair,†Advance on Wound Care, vol. 3, no. 10, pp. 647-661, 2014

P. Brainbridge, “Wound healing and the role of fibroblasts,†Journal of Wound Care, vol. 22, no. 8, pp. 407-412, 2013

M. Xue and C. J. Jackson, “Extracellular matrix reorganization during wound healing and its impact on abnormal scarring,†Advance on Wound Care, vol. 4, no. 3, pp. 119-136, 2015

P. Olczyk, L. Mancner, and K. Komosinska-Vasser, “The role of extracellular matrix components in cutaneous wound healing,†BioMed Research International, vol. 2014, pp. 1-8, 2014

T. A. Dechert, A. E. Ducale, S. I. Ward, and D. R. Yager, “Hyaluronan in human acute and chronic dermal wounds,†Wound Repair and Regeneration, vol. 14, pp. 252–258, 2006.

T. Tashiro, S. Seino, T. Sato, R. Matsuoka, Y. Masuda, and N. Fukui, “Oral administration of polymer hyaluronic acid alleviates symptoms of knee osteoarthritis : a double-blind, placebo-controlled study over a 12-month period,†The Scientific World Journal, vol. 2012, pp. 1-8, 2012.

R. Sahitha, “Effects of smokeless tobacco, betel quid and areca nut on oral mucosa,†IOSR Journal of Dental and Medical Sciences, vol. 13, no. 2, pp. 8-11, 2014

D. Rintu, M. Shinjini, M. Kaustab, P. Pramathadhip, P. S. Umesh, and E. R. Banerjee, “Anti-oxidant and anti-inflammatory activities of different varieties of piper leaf extracts (Piper betle L.),†Journal of Nutrition and Food Sciences, vol. 5, no. 5, pp. 1-15, 2015.

O. S. Kumari and N. B. Rao, “Phyto chemical analysis of Piper betle leaf extract,†World Journal of Pharmacy and Pharmacheutical Sciences, vol. 4, no. 1, pp. 699-703, 2014

L. T. Lien, N. T. Tho, D. M. Ha, P. L. Hang, P. T. Nghia, and N. D. Thang, “Influence of phytochemical in piper betle linn leaf extract on wound healing,†Burns Trauma, vol. 3, pp. 23-30, 2015

P. Pithayanukul, S. Nithitanakool, and R. Bavovada, “Hepatoprotective potential of extracts from seeds of Areca catechu and Nutgalls of Quercus infectoria,†Molecules, vol. 14, pp. 4987-5000, 2015

A. Vonna, R. Nurismi, and Misrahanum, †Wound healing activity of unguentum dosage form ethanolic extracts of Areca catechu l. nut in Mus musculus albinus,†Banda Aceh : Universitas Syiah Kuala, vol. 15, no. 2, pp. 28-36, 2015

T. Anggraini, A. Tai, T. Yoshino, and T. Itani, “Antioxidative activity and catechin content of four kinds of Uncaria gambir extracts from West Sumatra Indonesia,†African Journal of Biochemistry Research, vol. 5, no. 1, pp. 33-38, 2015

H. Katu, Sumintarti, I. K. Mattulada, R. Samad, M. Hatta, and S. As’ad, “Inhibitory concentration and minimum contact time gambir extract (Uncaria gambir Roxb.)againts bacterial growth Enterococcus faecalis,†International Journal of Science: Basic and Applied Research, vol. 27, no. 3, pp. 239-246, 2016

M. Yimam, Y. C. Lee, T. W. Kim, et al, “Analgesic and antiinflamatory effect of UP3005, a botanical composition containingtwo standardized extracts of Uncaria gambir and Morus alba,†Pharmacognosy Research, vol. 7, no. 5, pp. 39-46, 2015

S. R. P. Dewi, M. T. Kamaluddin, Theodorus, and R. Pambayun, “Anticariogenic effect of gambir (Uncaria gambir Roxb.) extract on enamel root surface exposed by Streptococcus mutans,†International Journal of Health Science and Research, vol. 6, no. 8, pp. 171-179, 2016.

N. N. Sazwi, T. Nalina, and Z. H. A. Rahim, “Antioxidant and cytoprotective activities of Piper betle, Uncaria gambir, and betel quid with and without calcium hydroxide,†BMC Complementary and Alternative Medicine, vol. 13, pp. 351-361, 2013

S. Taniguchi, K. Kuroda, N. Yoshikado, et al, “New dimeric flavans from gambir, an extract of Uncaria gambir,†The Japan Institute of Heterocyclic Chemistry, Okayama University, pp. 1-11, 2008.

D. Shalin and C. Nicholas, “Calcium hydroxide-based root salers: A review,“ Journal of Endodontic, vol. 12, no. 4, pp. 1-5, . 2009.

C. Bal, A. Alacam, T. Tuzuner, R. E. Tirali, and E. Baris, “Effects of antiseptics on pulpal healing under calcium hydoxide pulp capping: a pilot study,†European Journal of Dentistry, vol. 5, no. 3, pp. 265-272, 2011

J. W. M. Arts, K. Kramer, S. S. Arndt, and F. Ohi, “Sex differences in physiological acclimatization after transfer in Wistar rats,†Animals (Basel), vol. 4, no. 4, pp. 693-711, 2014

V. Dwivedi and S. Tripathi, “Review study on potential activity of Piper betle,†Journal of Pharmacognosy and Phytochemistry, vol. 3, no. 4, pp. 93-98, 2014.

A. A. Khalil, A. A. Rahman, M. R. Khan, A. Shahar, T. Mehmood, and M. Khan, “Essensial oil eugenol: source, extraction techniques, and neutraceutical perspectives,†RSC Advances vol. 2, pp. 32669, 2017

T. F. Bachiega, J. P. de Sousa, J. K. Bastos, and J. M. Sforcin, “Clove and eugenol in noncytotoxic concentration exertimmunomodulatory/anti-infalamatory action on cytokine production by murine macrophages,†Journal of Pharmacheutical and Pharmacology, vol. 64, no. 4, pp. 610-616, 2012

K. Y. Pin, A. L. Chuah, A. A. Rashih, et al, “Antioxidant and antiinflamatory activities of Betel leaves (Piper betle) from solvents with different polarities,†Journal of Tropical Forest Science, vol. 22, no. 4, pp. 448-455, 2010

G. P. Kamatou, I. Vermalk, and M. Viljoen, “Eugenol-from the remote Maluku islands to the International market place: A review of a remarkable and versatiles molecule,†Molecules, vol. 17, pp. 6953-6981, 2012

D. Rintu, M. Shinjini, M. Kauscab, P. Pramathadhip, P. S. Umesh, and E. R. Banarjee, “Anti-oxidant and anti inflammatory activities of different varieties of Piper Leaf extract (Piper Betle L),†Journal of Nutrition and Food Science, vol. 5, no. 5, pp. 1-16, 2015

N. A. Ghazali, A. Elmy, L. C. Yuen, et al, “Piper betle leave induces wound healing activity via proliferation of fibroblast and reducing 11b hydroxysteroid dehydrogenase 1- expression in diabetic rats,†Journal of Ayurveda Integrated Medicine, vol. 7, no. 4, pp. 198-208, 2016

E. C. Keat, S. S Razak, N. M. Fadil, et al, “The effect of Piper betle extract on the wound healing process in experimentally induced diabetic rats,†Clinical Therapy, vol. 161, no. 2, pp. 117-120, 2010

L. T. Lien, N. T. Tho, D. M. Ha, P. L. Hang, P. T. Nghia, and N. D. Thang, “Influence of phytochemicals in Piper betle Linn extract on wound healing. Burns and trauma,†vol. 3, pp. 23-30, 2015

K. C. Nilugal, K. Perumal, R. E. Ugander, and A. I. Chitton AI, “Evaluation of wound activity of Piper betle leaves and stem extract in experiment Wistar rats,†American Journal of Pharmtech Research, vol. 4, no. 3, pp. 1-6, 2014

A. Garg, P. Chaturvedi, and P. C. Gupta, “A review of the synthesis adverse effect of Areca nut or Betel nut,†Indian Journal of Medical and Pediatric Oncology, vol. 35, no. 1, pp. 3-9, 2014

J. Tham, G. Sem, E. Sit, and M. Tai, “The ethics of betel nut consumption in Taiwan,†Journal of Medical Ethics, vol. 43, no. 11, pp. 739-740, 2016

Q. Wu, Y. Yang, and J. E. Simon, “Qualitative and quantitative HPLC/MS determination of proanthocyanidins in areca nut (Areca catechu L.) by solvent extraction, its antioxidant activity and identification of polyphenols,†Journal of Science and Food Agriculture, vol. 93, no. 10, pp. 2580-2589, 2013

J. Sharafudheen, S. Goplakrishnan, P. Aneesh, and J. K. Mukkkadan, “Anti-inflammatory and antinociceptive activities of areca nut water extract,†International Journal of Innovative Pharmaceutical Science and Research vol. 3, no. 4, pp. 278-284, 2015

A. Hanna, S. Karan, and T. P Chatterjee, “Anti-inflammatory and analgesic activity of methanolic extract of areca seed collected from Areca catechu plant grown in Assam,†International Journal of Pharmaceutical Chemistry and Science, vol. 1, no. 2, pp. 690-698, 2012

P. L. Huang, C. W. Chi, and T. Y. Liu, “Effects of Areca catechu L. containing procyanidins on cyclooxygenase-2 expression in vitro and in vivo,†Food Chemistry and Toxicology, vol. 48, no. 1, pp. 306–313, 2010

R. R. N. Anthikat and A. Michael, “Anti-inflammatory and antioxidant effect of Areca catechu,†International Journal Pharmaceutical Science and Research, vol. 3, no. 6, pp. 2031-2037, 2012.

A. Vonna, Nurismir, and Misrahanum, “Wound healing activity of unguentum dosage form of ethanolic extract of Areca catechu L. nut in Mus musculus,†Journal of Natural, vol. 15, no. 2, pp. 28-36, 2015

D. K. Verma, M. Bharat, D. Nayak, T. Shanbhag, V. Shanbhag, and R.S. Rajput, “Areca catechu: effect of topical ethanolic extract on burn wound healing in albino rats,†International Journal of Pharmacology and Clinical Science, vol. 1, no. 3, pp. 74-78, 2012

M. Bharat, D. K. Verma, V. Shanbhag, R. S. Rajput, D. Nayak, and A. Amuthin, “Ethanolic effect of oral Areca catechu promotes burn wound healing in rats,†International Journal of Pharmacetical Sciences Review and Research, vol. 25, no. 2, pp. 145-148, 2014.

Rahmawati, Noveri, A. Fernando, and Wachyuni, “ Phenolic ingredients and antioxidant activity of dried gambier (Uncaria gambir (Hunter) Roxb) leaves’ extract,†Journal of Indonesia Chemical Acta,vol. 4, no. 1, pp. 1-6, 2013.

Rathee, Permender, H. Chaudhary, S. Rathee, et al, “Mechanism of action of flavonoids as anti-inflammatory agents: a review,†Inflammation and Allergy-Drug Targets, vol. 8, pp.229-235, 2009.

T. D. Ngunyen, P. T. Thuang, I. H. Hwang, et al, “Anti-hyperuricemic, anti-inflammatory and analgesic effect of Srieges beckia orientalis L resulting from the fraction with high phenolic content,†BMC Complimentary and Alternative Medicine, vol. 17, pp. 191-202, 2017

M. Yimam, Y.C. Lee, T. W. Kim, et al, “Analgesic and antiinflammatory effect of UP 3005, a botanical composition containing two standarized extracts og Uncaria gambie and Moris alba,†Pharmacognosy Research, vol. 7, no. 1, pp. 39-46, 2015

E. Riswana, S. Ningsih, and F. Fachrudin, “In vitro study of xanthine oxidase inhibitory of gambir (Uncaria gambir) Hunter Roxb extract,†Pharmacognosy Journal, vol. 9, no. 6, pp. 862-865, 2017

R. Pambayun, M. Gardjito, S. Sudarmadji, and K. R. Kuswanto, “Phenolic content and antibacterial properties of various extract of gambir (Uncaria gambir Roxb.),†Majalah Farmasi Indonesia, vol. 18, no. 3, pp. 141-146, 2007

N. S. Sumosa, Efrizal, and R. Resti, “The effect of gambir (Uncaria gambir R.) in burn wound healing in male white rats Mus musculus,†Journal Biologi Universitas Andalas, vol. 1, pp. 283-288, 2014.

D. Septiani, E. R. Yuslianti, and S, L. Nasroen, “Effect of ethanol gambir leaves (Uncaria gambir) compared with chlorhexidine gluconate 0,2% topical for wound healing on palate mucosal galur Wistar rats,†Dental Dentika Journal, vol. 18, no. 3, pp. 262-267, 2015.

National Center for Biotechnology Information, “Calcium hydroxide,†PubChem Database. http://pubchem.ncbi.nlm.nih.gov/compound/6093208. Accessed Dec, 19th, 2017)

R. P. De Freites, V. R. Greavatti, M. P. Alcalde, et al, “Effect of the association of non streroidal anti-inflammatory and antibiotic drugs on antibiofilm activity pH calcium hydroxide pastes,†Journal of Endodontic, vol. 43, no. 1, pp. 131-134, 2017

S. P. Karapinar, Y. Z. A. Ulum, B. Ozcelik, et al, â€The effect of N-acetylcystein and calcium hydroxide on TNF-b1 in lipopolysaccharide-activated macrophages,†vol. 68, pp. 48-54, 2016

A. Kasaj, B. Willershausen, M. Berakolar, H. Tekyatan, and A. Sculean, â€Effect of an oily calcium hydroxyde suspension on early wound healing after non surgical periodontal theraphy,†vol. 10, no. 1, pp. 72-76, 2006

Y. M. Ji, S. H. Joen, J. Y. Park, J. H. Chung, Y. H. Chuong, andP. H. Chuong, “Dental stem cell theraphy with calcium hydroxide in dental pulp capping,†Tissue Engineering, vol. 16, no. 6, pp. 1823-1835, 2010

Published

2019-12-27

How to Cite

Puspa Dewi, S. R., & Fatonah, A. (2019). The Effect of Betel Quid Extract on Wound Healing Process in Male Wistar Rats (Rattus norvegicus L.). Asian Journal of Applied Sciences, 7(6). https://doi.org/10.24203/ajas.v7i6.6035