Optimization of Culture Conditions for the Highest Lipid Production from some Oleaginous Fungi for Biodiesel Preparation


  • Thanaa Hamed Ali Microbial Chemistry Dept., Genetic Engineering and Biotechnology Division, National Research Center, Tahrir St., 12622 Dokki, Cairo
  • Dina H. El-Ghonemy


Biodiesel, Lipid, Oleaginous fungi, Trichoderma viride, optimization


Ten different fungal strains belonging to filamentous fungi were evaluated for their abilities to produce lipid. An oleaginous fungus strain Trichoderma viride-314 was grown the fastest and gave comparable high lipid content up to 20 %. Culture conditions were optimized for oil production by the above fungus in the medium supplemented  with  different  carbon  sources,  different  concentrations  of  the  best carbon source, different pH, temperature and incubation period. The experimental results  showed  that PD liquid  nutrient  medium  supported  relatively lipid accumulation with 50 g L-1  for dextrose concentration at the fifth day of growth.


Neither yeast extract nor sugar cane molasses supplement exhibits any significant effects on lipid production.   In addition, 28°C, 5.0, static conditions were the favorable for growth  temperature,  pH  value  of  cultivation  medium,  respectively.  The  GC/Ms analysis indicated that the n-hexane lipid fraction of T. viride was mainly composed of

30.01% Palmitoleic acid (C16:1), 23% Linoleic Acid (C18:2), 13% Linolenic acid (C18:3)  and  about  8%  Erucic  acid  (C22:1).  Furthermore,  the  GC  analysis  also showed that the total saturated fatty acids represented 8.3% while the value of the total unsaturated was 81.74%. These results giving possibility to use such this fungus strain to produce SCO in a low cost medium from economic point of view.


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How to Cite

Ali, T. H., & El-Ghonemy, D. H. (2014). Optimization of Culture Conditions for the Highest Lipid Production from some Oleaginous Fungi for Biodiesel Preparation. Asian Journal of Applied Sciences, 2(5). Retrieved from https://ajouronline.com/index.php/AJAS/article/view/1762