Molecular Characterization of Fusarium oxysporum isolates from Egypt using IGS Sequencing
Keywords:
Fusarium oxysporum, intergenic spacer region IGS, sequencing, tandem repeatsAbstract
Twenty isolates of Fusarium oxysporum were collected from different regions of Egypt and characterized using intergenic spacer (IGS) of the ribosomal DNA gene. The identified isolates were compared using sequences analyses on formae speciales level. The length of the amplified IGS region using universal primers CNL12 and CNS1 were about 2.6 kb in all F. oxysporum isolates. For specificity, oligonucleotide primers PNFo and PN22 produced a single DNA fragment of about 1.7 kb for each of the twenty isolates. Sequence cluster analysis using the neighbor-joining method showed that the twenty isolates divided into two clusters, cluster 1 contains isolates from El Fayoum and the remaining isolates on the other cluster. On the whole, there was no clear correlation between geographic origin or plant host and the sequence of IGS region. The results demonstrated the important role for utilization the IGS region as a molecular marker for the rapid and reliable diagnosis, identification and characterization of F. oxysporum pathogen in solanaceous crops in Egypt.
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References
Appel.D.Jand Gordon.T.R.1996. Relationships among pathogenic and nonpathogenic isolates of Fusarium oxysporum based on the partial sequence of the intergenic spacer region of the ribosomal DNA. Molecular Plant-Microbe Interaction. 9(2): 125-138
Abo K, Klein KK, Edel-Hermann V, Gautheron N, Dossahoua T, Steinberg C, 2005. High genetic diversity among strains of Fusarium oxysporum f. sp. vasinfectum from cotton in Ivory Coast. Phytopathology 95, 1391–6.
Baayen RP, O'Donnell K, Bonants PJM, Cigelnik E, Kroon LPNM, Roebroeck EJA, Waalwijk C., 2000 Gene genealogies and AFLP analyses in the Fusarium oxysporum complex identify monophyletic and non monophyletic formae speciales causing wilt and rot disease. Phytopathology 90:891-900
Balogun, O.S. 2007. Comparison of Fusarium oxysporumf.sp. lycopersici races 1, 2 and 3, and f. sp radicis-lycopersici based on the sequences of fragments of the ribosomal DNA intergenic spacer region. AJOL. 19(1):1-8
Booth, C. 1971. The genus of Fusarium. Commonwealth Mycological Institute. Kew, Surrey, U.K. 237.pp.
Cai G, Rosewich,Gale L, Schneider R.W., 2003. Origin of race 3 of Fusariumoxysporum f. sp. lycopersici at a single site in California. Phytopathology 93, 1014–22.
Edel V, Steinberg C, Avelange I, Laguerre G, Alabouvette C, 1995. Comparison of three molecular methods for the characterization of Fusarium oxysporum strains. Phytopathology 85, 579–85.
Edel V, Steinberg C, Gautheron N, Alabouvette C, 1997. Populations of nonpathogenic Fusarium oxysporum associated with roots of four plant species compared to soilborne populations. Phytopathology 87, 693–7.
Edel V, Steinberg C, Gautheron N, Recorbet G, Alabouvette C, 2001. Genetic diversity of Fusarium oxysporum populations isolated from different soils in France. FEMS Microbiology Ecology 36, 61–71.
Edel-Hermann, V.; Gautheron, N. and Steinberg, C. 2011. Genetic diversity of Fusarium oxysporum and related species pathogenic on tomato in Algeria and other Mediterranean countries. Plant Pathology. 61:787–80
Erlangung des Grades, I.D. 2010. Endophytic Fusarium oxysporum: Phylogeny and induced defense responses in banana plants against Radopholus similis. Ph.D. Thesis, Highagricultural facultyof the Rheinische Friedrich-Wilhelms-UniversitättoBonn.
Grattidge, R. and O’Brien, O.1982. Occurrence of a third race of Fusarium wilt of tomatoes in Queensland. Plant Disease 66, 165–6.
Jordão do Amaral, D.O.; Almeida, C.M.; Malafaia, C.B.; Bastos da Silva, M.L.R.; Santos Correia, M.T. ; Menezes Lima, V.L. and Silva, M.V. 2013.Identification of races 1, 2 and 3 of Fusarium oxysporum f. sp. lycopersici by molecular markers. African Journal of Microbiology Research. 7(20):2324-2331
Kim, H.; Choi, Y. and Min, B. 2001. Variation of the intergenic spacer (IGS) region of ribosomal DNA among Fusarium oxysporum formae specialis. The Journal of Microbiology. 39(4): 265-272
Kim, H.J. and Min, B.R. 2004. Nucleotide divergence analysis of IGS region in Fusarium oxysporum and its formae speciales based on the sequence. Mycobiology.32(3):119-122
Lievens, B.; Rep, M. and Thomma, B.P.H.J. (2008). Recent developments in the molecular discrimination of formae speciales of Fusarium oxysporum. Pest ManagSci 64: 781–788
Lori, G., Edel-Hermann, V., Gautheron, N., and Alabouvette, C. 2004. Genetic diversity of pathogenic and nonpathogenic populations of Fusarium oxysporum isolated from carnation fields in Argentina. Phytopathology. 94:661-668.
Mbofung, G.Y., Hong, S.G., and Pryor, B.M. 2007. Phylogeny of Fusarium oxysporumf. sp. lactucae inferred from mitochondrial small subunit, elongationfactor 1-_, and nuclear ribosomal intergenic spacer sequence data. Phytopathology, 97(1): 87–98.
O'Donnell, K., Gueidan, C., Sink, S., Johnston, P.R., Crous, P.W., Glenn, A., et al. 2009. A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex. Fungal Genet. Biol. 46(12): 936–94
Salvador, M.; Belen, P.; Miguel,J.; Covadonga, V. and Gonzalez-Jaen, M.T. 2013. Structural variation and dynamics of the nuclear ribosomal intergenic spacer region in key members of the Gibberella fujikuroi species complex. Genome. 56: 205-2013
Srinivasan,K.; Gilardi, G.; Spadaro, D.; Gullino M. L. and A. Garibaldi.2010. Molecular characterization through IGS sequencing of formae speciales of Fusarium oxysporum pathogenic on lamb’s lettuce. Phytopathol. Mediterr. 49: 309–320
Zambounis, A.G.; Paplomatas, E.J.; Tsaftaris. A.S. 2006. Intergenic spacer – RFLP analysis and direct quantification of Australian Fusarium oxysporum f. sp. vasinfectum isolates from soil and cotton infected tissues. Plant Disease. 91(12): 1564-1573
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