Carbon Fixation of a Microalgae <em>scenedesmus sp.</em> Pilot Culture under Different Nutritional Inputs

Authors

  • José F. Reyes , Universidad de Concepción, Chile
  • Cristian Inostroza University of Almeria, Spain

DOI:

https://doi.org/10.24203/ajas.v8i4.6290

Keywords:

microalgae, abatement, raceway, flue gas, algal oil

Abstract

A culture of the microalgae Scenedesmus sp. in a pilot closed raceway photo bioreactor (CRPB), with flue gas injection from a diesel engine was implemented. Two different nutritional medium, Z-8 and EPA were used to feed the culture growth that was monitored in terms of total and partial biomass productivity, carbon fixation and oil production during nine days. The system was sequentially sampled measuring the gas flow and concentration of the injected CO2, the amount of biomass harvested and the concentration of CO2 in the degassing flow. In addition, the pH was measured in the culture to assess the amount of CO2 instantaneously dissolved. The results at the steady state, showed a carbon fixation efficiency ranging between 21.6 % and 44.9 %, and that the Z-8 medium was clearly better than the EPA in terms of CO2 capture and therefore biomass and oil production. A continuous increasing of oil content in microalgae biomass up to 6.6 % dry basis, with maximum oil production rate of 2.27 g m-3d-1 was observed, with a maximum rate of biomass production of 44.97 g m-3d-1 and a maximum carbon capture rate of 2.27 g m-3d-1 was achieved with the culture medium Z-8.

References

Mata, T.M., Martins, A.A., Caetano, N.S., “Microalgae for biodiesel production and other applications: A review”, Renewable and Sustainable Energy Reviews 14: 217-232, 2009.

P, Joannis-Cassan, C., Duran, E., Isambert, A., “Commercial applications of microalgae”, J Biosci Bioeng. 101: 87–96, 2006.

Benemann, J.R., “Technology Roadmap - Biofixation of CO2 and Greenhouse Gas Abatement with Microalgae”, Final Report to the U.S. Dept. of Energy, Morgantown – Pittsburgh, January 14, 2003. Available at http://www.ieagreen.org.uk/, 2003.

Woerstz, I.C., Fulton, L., Lundquist, T.J., “Nutrient removal & greenhouse abatement with CO2-supplemented algal high rate ponds”, Paper written for the WEFTEC annual conference, Water Environment Federation, October 12-14, 2009, Orlando, Florida, pp.13, 2009.

Monari, Ch., Righi, S., Olsen, S.I., “Greenhouse gas emissions and energy balance of biodiesel production from microalgae cultivated in photobioreactors in Denmark: a lifecycle modeling”, Journal of Cleaner Production 112: 4084-4092, 2016.

Maity, J.P., Bundschuh, J., Chen, C.Y., Bhattacharya, P., “Microalgae for third generation biofuel production, mitigation of greenhouse gas emissions and wastewater treatment: Present and future perspectives - A mini review”, Energy 78:104-113, 2014.

Matsunaga, T., Matsumoto, M., Maeda, Y., Sugiyama, H., Sato, R., Tanaka, T., “Characterization of marine microalga, Scenedesmus sp. strain JPCC GA0024 toward biofuel production”, Biotecnol Lett. 31:1367-1372, 2009.

Quevedo, C.O., Morales, S.V., Acosta, A.C., “Crecimiento de Scenedesmus sp. en diferentes medios de cultivo para la producción de proteína microalgal”, VITAE. Revista de la Facultad de Química Farmacéutica UdeA. 15(1):23-29, 2008.

Lara, M.J., “Producción de microalgas Scenedesmus spinosus en un fotobioreactor tubular”, Memoria de Titulo. Ing. Agroind. Universidad de Concepción. Fac. Ing. Agr. Chillán. Chile, 2011.

Grobbelaar, J.U., “Algal nutrition. In: A. Richmond, Editor, Handbook of microalgal culture: biotechnology and applied phycology”, Blackwell (2004), pp. 97–115, 2004.

Camacho Rubio, F.G., Fernández, A., García Camacho, F., Sánchez Pérez, J.A., Molina Grima, E., “Prediction of dissolved oxygen and carbon dioxide concentration profiles in tubular photobioreactors for microalgal culture”, Biotechnol Bioeng 62: 71–86, 1999.

Carvalho, A.P., Meireles, L.A., Malcata, F.X., “Microalgal reactors: a review of enclosed system designs and performances”, Biotechnol Prog 22: 1490–1506, 2006.

Contreras-Flores, C., Peña-Castro, J.M., Flores-Cotera, L.B., Cañizares-Villanueva, R.O., “Avances en el diseño conceptual de fotobiorreactores para el cultivo de microalgas”, INCI, ago. 2003, vol.28, no.8, p.450-456. ISSN 0378-1844, 2003.

Molina Grima, E., Fernández, J., Acién Fernández, F.G., Chisti, Y., “Tubular photobioreactor design for algal cultures”, Biotechnol 92: 113–131, 2001.

Kumar, A., Ergas, S., Yuan, X., Sahu, A., Zhang, Q., Dewulf, J., Malcata, X., Langenhove, H.V., “Enhanced CO2 fixation and biofuel production via microalgae: recent developments and future directions”, Trends in Biotechnology 28: 371-380, 2010.

Olaizola, M., Bridges, T., Flores, S., Griswold, L., Morency, J., Nakamura, T., “Microalgal removal of CO2 from flue gases: CO2 capture from a coal combustor”, U.S. Department of Energy under award No. DE-FC26-00NT40934, 2006.

Yoo, C., Jun, S.Y., Lee, J.Y., Ahn, C.Y., Oh, H.M., “Selection of microalgae for lipid production under high levels carbon dioxide”, Bioresour. Technol. 101(1): S71-S74, 2010.

Hernández-Reyes, E., Martinez-Roldan, A., Cañizares-Villanueva, R., “Contenido de lípidos en dos especies del género Scenedesmus para la eventual producción de bioenergéticos”, En: XIII Congreso Nacional de Biotecnología y Bioingeniería y VII Simposio Internacional de Producción de Alcoholes y Levaduras:21 a 26 de junio, 2009 Acapulco, Guerrero, Hotel Fairmont Acapulco Princess, 2009.

Ho, S.H., Chen, W.M., Chang, J.S., “Scenedesmus obliquus CNW-N as a potencial candidate for CO2 mitigation and biodiesel production”, Bioresour. Technol. 101: 8725-8730, 2010.

Moraes, L., Martins da Rosa, G., Barcelos Cardias, B., Oliveira dos Santos, L., Vieira Costa, J.A., “Microalgal biotechnology for greenhouse gas control: Carbon dioxidefixation by Spirulina sp. at different diffusers”, Ecological Engineering 91: 426-431, 2016.

Hernández, A.P., “Desarrollo y habilitación de un controlador y registrador, para un reactor de microalgas”, Habilitación Profesional. Ing. Civil Agric. Universidad de Concepción. Fac. Ing. Agr. Chillán. Chile, 2010.

Guschina, I.A., Harwood, J.L., “Lipids and lipid metabolism in eukaryotic algae”, Prog Lipid Res 45: 160–186, 2006.

Downloads

Published

2020-08-28

How to Cite

Reyes, J. F., & Inostroza, C. (2020). Carbon Fixation of a Microalgae <em>scenedesmus sp.</em> Pilot Culture under Different Nutritional Inputs. Asian Journal of Applied Sciences, 8(4). https://doi.org/10.24203/ajas.v8i4.6290

Issue

Section

Articles