Antioxidant Activities of Ethyl acetate Extract and Hexane Extract of Lelak (Uvaria rufa Blume) Leaves
Keywords:
Antioxidant, DPPH, Uvaria rufa, IC50Abstract
Uvaria rufa Blume is one of the main power plant that has natural antioxidants that are known to inhibit the scavenging free radicals.  The present study was to evaluate antioxidant activity of ethyl acetate and hexane extracts of leaves of Uvaria rufa Blumes.  Measurement of antioxidant activity carried out using the DPPH method.  DPPH radical scavenging activity of Uvaria rufa Blume of hexane extract and ethyl acetate extract tested showed lower activity than that of a standard compounds: ascorbic acid (IC 50 = 3.781 µg/ml ). Extracts of ethyl acetate has strong antioxidant activity which IC50 values was 57.89µg/ml respectively and  extracts of hexane has weak antioxidant activity which IC50 values was 87,292 µg/ml respectively.
Â
Â
References
• Bingtao, L. and Michael G. G. 2011. UVARIA Linnaneus. FI. China 19: 674-676
• Cahyadi, W. 2008. Food Additive. 2nd Edition. Bumi Aksara. Jakarta
• Cairns, D. 2008. Digest Pharmaceutical Chemistry. 2nd Eition . EGC. Jakarta
• Can-Ake, R., Gilda, E.R., Filogonio, M.P., and Luis, M.P. 2004. Bioactive terpenoids from roots and leaves of Jatropha gaumeri. Rev Soc QuÃm Méx 48: 11-14.
• Daniel. 2010. Isolasi dan Identifikasi Senyawa Flavonoid Pada Fraksi Etil Asetat dari Daun Tumbuhan Sirih Merah (Piper crocatum Ruiz dan Pav). Volume 9. Nomor 10. FMIPA Universitas Mulawarman. Samarinda
• Didik, G dan Sri Mulyani. 2004.Phatmacology. Jilid I. Swadaya. Jakarta
• Deepralard, Khanittha, dkk. 2009. Flavonoid Glycosides from the Leaves of Uvaria rufa with Advanced Glication end-Product Inhibitory Activity. Faculty of Pharmaceutical Sciences, Culalongkorn UniversityBangkok.Http://161.200.184.9/tjps/ContentVol33No2_3/V33-2-3Art%203%20pp8490.pdf. (Accessed on May 17, 2014).
• Edhisambada. 2011.Antioxidant Activity Test Methods Radikal 1,1-difenil-2-pikrilhidrazil (DPPH). https://edhisambada.wordpress.com/2011/02/22/ metode-uji-aktivitas-antioksidan-radikal-11-difenil-2-pikrilhidrazil-dpph/ ( Accesed on Feb2, 2015)
• Gandjar, I. G. dan Rohman, A. 2007. Pharmaceutical Analysis. Pustaka Pelajar. Yogyakarta
• Gafur, M. A.., Isa, I., dan Bialangi, N. 2013. Isolation and Identification of Flavonoid Compounds of Leaf Jamblang (Syzygium cumini). http//:repository.ung.ac.id/get/simlit_res/1/458 ( Accesed on June, 25, 2015)
• Harborne, J. B. 1987. Phytochemicals method. 2nd Edition. ITB Bandung.
• Kedare, S. B. and Singh, R. P. 2011. Genesis and Development of DPPH Method of Antioxidant Assay. J. Food. Sci. Technol. 48(4):412–422
• Marx, J.L. 1985. Oxygen Free Radicals Linked to Many Disease, dalam Science, 235 : 529-531
• Orozco, M.CB, Rasco, J.J, Cedillo, F.D, Torres, R.S, 2013, Chemical composition and antioxidant ability of the crude extract of Sedum praealtum flowers. J.Food Agric, 25(10) 778-784
• Rohman, A., Riyanto, S., Utari, D., 2006, Antioxidant activities, total phenolic and flavonoid contents of ethyl acetate extract of Mengkudu (Morinda citrifolia, L) fruit and its fractions. Journal Pharmacy Indonesia
• Tan Hoan dan Kirana Rahardja. 2007. Medications Important . 6th Edition . Elex Media Komputindo.Jakarta
• Winarsi, H.2007. Natrural Anti- oxidant and free radical : Potential and Its Application in Health. Kanisius. Yogyakarta
• Wijayakusuma H. 2000. Potential medicinal plants native to Indonesia as a health product [Online cited 2009 Sept 23]; Available from: URL:http://digilib.batan.go.id/eprosiding/File%20Prosiding/Kesehatan/ Risalah%202000/2000.pdf. (Accesed on feb 15, 2015)
• Zou, Y., Lu, Y. and Wei,D., 2004, Antioxidant activity of Flavonoid-rich extract of Hypericum perforatum L in vitro, J. Agric. Food Chem,52, 5032-5039.
Downloads
Published
Issue
Section
License
Copyright © The Author(s). This article is published under the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
