Thai Medicinal Plant Recipes: Evaluative Study Onanti-proliferative on Cancer Cell Lines and Anti-oxidant Activities
Keywords:
Anti-proliferative, Anti-oxidation, Free radical scavenging, Lipid peroxidation, Metal chelatingAbstract
This research deals with the in vitro evaluative study of six Thai medicinal plant recipes (i.e. TCRs 1-6) individually undergoing four extraction methods including aqueous cold maceration (AQC), aqueous hot maceration (AQH), ethanolic cold maceration (EtCM) and ethanolicsoxhlet extraction (EtSE), for the antiproliferative activity on four cancer cell lines (i.e. DU145, MCF-7, HeLa, and B16F10) using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and the antioxidant behavior using various colorimetric methods. The plant extracts of most of the six recipes exhibited IC50>100µg/ml on the cancer cell lines, except for TCR5 whose AQC and EtCM respectively showed the IC50 of 53.89 and 75.85µg/ml on B16F10. Besides, the TCR4-AQC and AQH extracts exhibited the best scavenging performance with an SC50 of 0.02 mg/ml, which was 1.5 times higher than vitamin C with an SC50 of 0.03 mg/ml. In the inhibition of lipid peroxidation activity, LC50 of the TCR3-EtSE extract was 0.43mg/ml, approximately 3.4 times the reference standard Vitamin E with an LC50 of 1.44 mg/ml. The findings also revealed that the aqueous extractions of TCR1 had the best ferric ion chelating activity with MC50 of 0.28 - 0.29 mg/ml, roughly 1.6 times higher than the reference standard EDTA with an MC50 of 0.45 mg/ml. In addition, the phenolic contents were highest in the TCR1-AQC extract (2.98 mg/g GAE) and lowest in the TCR3-AQH extract (0.25mg/g GAE). The results also indicated a mild positive correlation between scavenging activity, lipid peroxidation inhibition and total phenolic content. Due to the significantly greater antioxidant efficacy vis-à -vis the antiproliferative activity, the proposed recipes could suitably be applied to the treatment of oxidant-related ailments.
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