Spraying with Commercial Fertilizers beside Effects of the Bio-nitrogen and Different Levels of Mineral Nitrogen on Growth, Yield and Quality of Broccoli


  • M. F. Zaki
  • Mona E. El-Azab Mona E. El-Azab Soil and water Dept. National Research Center, Dokki
  • S. A. Abou Sedera
  • M. M. Abou El-Magd


Foliar application, bio-nitrogen, amino magnical, broccoli


The aim of this study was to analyse the effect of foliar application of  amino magnical as commercial fertilizer beside the effect of bio-nitrogen and different mineral nitrogen on plant growth and yield of broccoli. Two field experiments were conducted at El- Nubaria, Beheira Governorate, Egypt during 2013/2014 and 2014/20015. Experimental design comprised three replicates. The bio-nitrogen treatments were used Microbin, Serialen and Azotobacter and different levels of mineral nitrogen. Amino-Magnical used as foliar application (100% N recommended dose + without spray of amino magnical; 50% N recommended dose + one spray; 50% N recommended dose + two sprays and 50% N recommended dose + three sprays). The  results showed that the application of Microbin increases the vegetative growth expressed as plant height, leaves number as well as fresh and dry weight of leaves, stems, apical heads and total plant compared with Serialen and Azotobacter treatments. Higher values of the abovementioned characteristics were also obtained with 100% N recommended dose without foliar application or 50% N recommended dose + One spray of amino magnical. Lower values of vegetative growth were obtained with Serialen or the other fertilization treatments. Total yield of apical heads and their physical quality expressed as (diameter, height and fresh weight) and their chemical quality recorded higher values with Microbin treatment compared with Serialen and Azotobacter treatments. In addition, treatments 100% N recommended dose without foliar application or 50% N recommended dose + one spray of amino magnical recorded the highest values of total yield and quality of apical heads compared with the other fertilization treatments. With respect to the interaction, Microbin treatment plants receiving 100% N recommended dose or 50% N recommended dose + one spray of amino magnical recorded the higher values of the vegetative growth, total yield of apical heads and their quality. While, the other interaction treatments gave  lower means of vegetative growth, total yield of apical heads and quality. The lowest values of vegetative growth, total yield of apical heads and their quality were obtained with Serialen and Azotobacter treatments receiving 50% N recommended dose + three sprays of amino magnical.


Abou El-Magd, M. M.; A. M. El-Bassiony and Z. F. Fawzy "Effect of organic manure with or without chemical fertilizers on growth, yield and quality of some varieties of broccoli plants." Journal of Applied Sciences Research, 2 (10): 791-798. 2006.

Abou El-Magd, M. M.; Hoda, A. Mohamed and Z. F. Fawazy " Relationship growth, yield of broccoli with increasing N, P or K ratio in a mixture of NPK fertilizers (Brassica oleracea var. italica plenck)." Annals of Agric. Sc., Moshtohor, 43 (2): 791-805.2005.

Abou El-Magd, M. M.; Omaima M. Sawan; M. F. Zaki and Faten S. Abd Ellall "Productivity and quality of two Broccoli cultivars as affected by different levels of nitrogen fertilizers." Australian Journal of Basic and Applied Science, 4 (12): 6125-6133.2010.

Abou El-Magd, M.M.; M.F. Zaki and S.A. Abo Sedera "Effect of Bio-Nitrogen as a Partial Alternative to Mineral-nitrogen Fertilizer on Growth, Yield and Head Quality of Broccoli (Brassica oleracea L. Var. Italica)." World Applied Sciences Journal 31 (5): 681-691.2014.

Agarwal, A.; S. Gupta and Z. Ahmed "Nitrogen nutrition and plant density influencing marketable head yield of broccoli in cold arid desert of Ladakh". Acta- Horticulturae, 756: 299-307.2007.

Aires, A.; E. Rosa; R. Carvalho; S. Haneklaus and E. Schnug " Response of nitrogen and sulfur fertilization on the mineral composition of broccoli sprouts. "Journal of Plant Nutrition, 30(7/9): 1035-1046.2007.

Association of Official Agricultureal Chemists (A.O.A.C.). " Association methods of analysis of the Official Analytical Chemists.12 th Ed.," published by A. O. A. C., Washington. D. C. 20044, U.S.A.1975.

Association of Official Agricultural Chemists (A.O.A.C.). "Association methods of analysis of the Official Analytical Chemists." Published by A.O.A.C., Washington. D. C., U.S.A.1980.

Black, C. A.; D. D. Evans; L. E. Ensminger; G. L. White and F.E. Clark "Methods of soil analysis". Part 2, pp: 1-100, Agron. Inc. Madison. Wisc., USA.1981.

Brown, J. D. and O. Lilleland " Rapid determination of potassium and sodium in plant material and soil extracts by flame photometry." Proc. Amer. Soc. Hort. Sci., 48: 341-346.1946.

Chaterjee, B.; P. Ghanti; U. Thapa and P. Tripathy " Effect of organic nutrition in sprouting broccoli (Brassica oleraceae var. italica Plenck)." Vegetable science, 33(1): 51-54.2005.

Decoteau, D. R. " Vegetable crops. Upper company. "New Jersey, U.S. A.2000.

Devi, H. J.; T. K. Maity and N. C. Paria. "Effect of different sources of nitrogen on yield and economics of cabbage." Environment and Ecology, 21(4): 878-880.2003.

El-Shakry, M. F. Z. " Effect of bio-fertilizers, nitrogen sources and levels on vegetative growth characters, yield and quality and oil yield of sweet fennel". Ph.D. Thesis, Fac. Agric. Cairo Univ., Egypt.2005.

Fawzy, Z. F.; M.A. El-Nemr and S. A. Saleh " Influence of levels and methods of potassium fertilizer application on growth and yield of eggplant." Journal of Applied Sciences Research, 2007; 3(1): 42-49. 2007.

Fawzy, Z.F.; A.M. El-Bassiony; A.G. Behairy and Y.I. Helmy " Effect of foliar spraying by some bio and organic compounds on growth, yield and chemical composition of snap bean plants." Journal of Applied Sciences Research, 2010; 6(12):2269-2274.2010.

Gadallah, F. M. and T. A. El-Masry “Onion growth and yield as affected by bio-fertilizationâ€. Annals Agric. Sci. Moshtohor, vol. 44, 2006, pp. 987–1005.2006.

Hanafy, A. H., N. F. Kheir, and N. B. Talaat. “Physiological studies on reducing the accumulation of nitrate in Jew's mallow (Corchorus olitorius) and radish (Raphanus sativus L.)â€. Bull. Fac. Agric., Cairo Univ., vol. 48, 1997, pp. 25–64. 1997.

Hassan H.A., Hassan XMY, Abou El-Magd MM, Anwar MT " Effect of different phosphorus fertilizer rates and foliar spray with some commercial nutrients on growth and yield of broccoli grown in sandy soils."J Appl Sci Res. 9:2052-2062.2013.

Ibrahim, A. N. and I. M. Abd El-Aziz. “Solubilization of rock phosphate by streptomycinâ€. Agr. Talajton, vol. 26, 1977, pp. 424–434. 1977.

Jackson, M. L. " Soil Analysis. Constable Co". Ltd.,London, pp:1-15.1973.

Jones, R. B.; M. Imsic; P. Franz; G. Hale and R. B. Tomkins "High nitrogen during growth reduced glucoraphanin and flavonol content in broccoli (Brassica oleraceae var. italica) heads." Australian Journal of Experimental Agriculture, 47(12): 1498-1505.2007.

Koch, F. C. and T. L. McMeekin " The chemical analysis of food and food products." J. Amer. Chem. Sec., 46: 2066.1924.

Mahendran, P. P. and N. Kumar " Effect of organic manure on cabbage cv. Hero (Brassica oleraceae var. capitata L.). "South Indian Horticulture, 45 (5/6): 240-243.1997.

Manivannan, M. I. and J. P. Singh. "Effect of bio-fertilizers on the growth and yield of sprouting broccoli (Brassica oleraceae var. Italica Plenck) under Allahabad agro-climatic conditions." Bioved; 15 (1/2): 33-36.2004.

Marschner, H. C. "Mineral nutrition in higher plants. Academic Press, Harcourt Brace Jovanovish," Publishers, pp., 674.1986.

Marschner, H. C. " Mineral nutrition in higher plants. Second Edition, "Academic Press Limited Text Book, pp., 864.1996.

Osman, A. S. “Effect of partial substitution of mineral-N by bio-fertilization on growth, yield and yield components of potatoâ€. The 3rd Conf. Sustain. Agric. Develop., Fac. Agric., Fayoum Univ., 12–14 Nov., pp. 381–396. 2007.

Rakesh, S.; S. N. S. Chaurasia and S. N. Singh " Response of nutrient sources and spacing on growth and yield of broccoli (Brassica oleraceae var. italica Plenck). "Vegetable science, 33(2): 198-200.2006.

Saad, M. Howladar, Mostafa M. Rady, and Ashraf S. Osman " Effect of bio-nitrogen as a partial alternative to mineral-nitrogen fertilizer on growth, nitrate and nitrite contents, and yield quality in Brassica oleracea L." International Journal of Agricultural Science and Engineering, 7 (7): 482-487.2013.

Sanaa, A.M.Z.; S.I. Ibrahim and H.A.M.S. Eldeen " The effect of naphthalene acetic acid (NAA) and salicylic acid on growth, fruit setting, yield and some correlated components in dry bean." Annals of Agric. Sci. Cairo, 46(2):451-463.2001.

Sanwal, S. K.; K. Laxminarayana; D. S. Yadav; N. Rai and R. K. Yadav " Growth, yield and dietary antioxidants of broccoli as affected by fertilizer type. "Journal of Vegetable Science, 12(2):13-26.2006.

Sanwal, S. K.; N. Rai; D. S.Yadav and R. K. Yadav " Compositional difference of stem and floral portions of broccoli under different organic manures and natural growth promoters. "Vegetable Science, 32 (1): 73-75.2005.

Sharma, K. C. " Influence of integrated nutrient management on yield and economic in broccoli (Brassica oleraceae var. italica). "Vegetable Science, 27(1):62-63.2000.

Singh, V.N. and S.S. Singh, "Effect of inorganic and bio-fertilizers on production of cauliflower (Brassica oleracea L. var. botrytis)". Vegetable-Science, 32(2): 146-149.2005.

Snedecore, W. C. and W. G. Cochran " Statistical Methods. 7 th ed., 2 nd printing." The Iowa State Univ. Press, Ames, Iowa, U.S.A.1980.

Talalay, P. and J. W. Fahey " Phytochemicals from cruciferous plants protect against cancer by maculating carcinogen metabolism. "American Soc. Nutr. Sci., 23: 3027-3033.2001.

Trough, E. and A. H. Meyer "Improvement in denies colorimetric method for phosphorus and arsenic. "Ind. Eng. Chem. Anal. Ed. 1: 136-139. 1939.

Wang, S.S. and R.H. Kale "Effect of microbial inoculants with graded levels of inorganic on broccoli and lettuce. "Journal of Soils and crops, 14(1): 18-21.2004.

Zaki, M. F.; A. A. M. Abdelhafez and Camilia Y. Eldewiny ". Influence of bio-fertilization and nitrogen sources on growth, yield and quality of broccoli (Brassica oleracea Var. Italica). "Egyptian Journal of Applied Sciences, 24 (3): 86-111.2009.

Zaki, M.F.; A. S. Tantawy; S.A. Saleh and Yomna, I. Helmy " Effect of bio-fertilization and different levels of nitrogen sources on growth, yield components and head quality of two broccoli cultivars." Journal of Applied Sciences Research, 8 (8): 3943 – 396.2012.




How to Cite

Zaki, M. F., El-Azab, M. E., Abou Sedera, S. A., & Abou El-Magd, M. M. (2016). Spraying with Commercial Fertilizers beside Effects of the Bio-nitrogen and Different Levels of Mineral Nitrogen on Growth, Yield and Quality of Broccoli. Asian Journal of Applied Sciences, 4(4). Retrieved from https://ajouronline.com/index.php/AJAS/article/view/3972