Functional Ingredients Extraction from Citrus <i>Genkou </i>by Supercritical Carbon Dioxide

Authors

  • Yukihiro Kawamoto
  • Yuriko Suidou Nagoya University
  • Takuya Suetsugu
  • Arata Takamizu
  • Masahiro Tanaka
  • Munehiro Hoshino
  • Wahyu Diono
  • Hideki Kanda
  • Motonobu Goto

Keywords:

Citrus genkou, Flavonoid, Supercritical carbon dioxide, Hesperidin, Nobiletin, Tangeretin

Abstract

Citrus genkou fruit was known as one of common sources for juice production. As residues, the citrus genkou peels has been discarded without any treatments. In this work, the citrus genkou peels were subjected as a starting material to extract flavonoids by using supercritical carbon dioxide (SC–CO2). The extractions were carried out at temperatures of 40 – 80 oC and pressures of 10 – 30 MPa. The high-performance liquid chromatography analysis showed that hesperidin, nobiletin, and tangeretin were extracted from wet citrus genkou peels. The results showed that the extraction by using SC–CO2 could be accomplished without compromising the organoleptic characteristics because SC–CO2 could be directly applied into matrix without any treatment.

 

 

References

Jung, UJ., Lee, M., Jeong, K., Choi, M., “The hypoglycemic effects of hesperidin and naringin are partly mediated by hepatic glucose-regulating enzymes in C57BL/KsJ-db/db miceâ€, J. Nutr., vol. 134, no. 10, pp. 2499-2503, 2004.

Mahmoud, AM., Ashour, MB., Abdel-Moneim, A., Ahmed, OM., “Hesperidin and naringin attenuate hyperglycemia-mediated oxidative stress and proinflammatory cytokine production in high fat fed/streptozotocin-induced type 2 diabetic ratsâ€, J. Diabetes Complications, vol. 26, no. 6, pp. 483–490, 2012.

Yamamoto, Y., Shioda, N., Han, F., Moriguchi, S., Nakajima, A., Yokosuka, A., Mimaki, Y., Sashida, Y., Yamakuni, T., Ohizumi, Y., Fukunaga, K., “Nobiletin improves brain ischemia-induced learning and memory deficits through stimulation of CaMKII and CREB phosphorylationâ€, Brain Res., vol. 1295, pp. 218-229, 2009.

Nakajima, A., Ohizumi, Y., Yamada, K., “Anti-dementia Activity of Nobiletin, a Citrus Flavonoid: A Review of Animal Studiesâ€, Clin Psychopharmacol Neurosci., vol. 12, no. 2, pp. 75-82, 2014.

Hirano, T., Abe, K., Gotoh, M., and Oka, K., “Citrus flavone tangeretin inhibits leukaemic HL-60 cell growth partially through induction of apoptosis with less cytotoxicity on normal lymphocytesâ€, Br. J. Cancer, vol. 72, no. 6, pp. 1380–1388, 1995.

Wang, L., Wang, J., Fang, L., Zheng, Z., Zhi, D., Wang, S., Li, S., Ho, CT., Zhao, H., “Anticancer Activities of Citrus Peel Polymethoxyflavones Related to Angiogenesis and Othersâ€, Biomed. Res. Int., vol. 2014, ID 453972, 2014, doi: 10.1155/2014/453972.

Manthey, JA., Guthrie, N., “Antiproliferative Activities of Citrus Flavonoids against Six Human Cancer Cell Linesâ€, J. Agric. Food Chem., vol. 50, no. 21, pp. 5837-5843, 2002.

Azmir, J., Zaidul, ISM., Rahman, MM., Sharif, KM., Mohamed, A., Sahena, F., Jahurul, MHA., Ghafoor, K., Norulaini, NAN., Omar, AK M., “Techniques for extraction of bioactive compounds from plant materials: A reviewâ€, J. Food Eng., vol. 117, no. 4, pp. 426–436, 2013.

Capuzzo, A., Maffei, ME., Occhipinti, A., “Supercritical Fluid Extraction of Plant Flavors and Fragrancesâ€, Molecules, vol. 18, no. 6, pp. 7194-7238, 2013.

Hoshino, R., Wahyudiono, Machmudah, S., Kanda H., Goto, M., “Simultaneous Extraction of Water and Essential Oils from Citrus Leaves and Peels Using Liquefied Dimethyl Etherâ€, J. Nutr. Food Sci., vol. 4, no. 5, pp. 301, 2014.

Manthey, JA., Grohmann, K., “Concentrations of Hesperidin and Other Orange Peel Flavonoids in Citrus Processing Byproductsâ€, J. Agric. Food Chem., vol. 44, no. 3, pp. 811–814, 1996.

Boye, JI., Arcand, Y., “Green Technologies in Food Production and Processingâ€, Springer Science+Business Media, LLC, New York, 2012.

Machmudah, S., Martin, A., Sasaki, M., Goto, M., “Mathematical modeling for simultaneous extraction and fractionation process of coffee beans with supercritical CO2 and waterâ€, J. Supercrit. Fluids, vol. 66, pp. 111–119, 2012.

Tomita, K., Machmudah, S., Wahyudiono, Fukuzato, R., Kanda, H., Quitain, AT., Sasaki, M., Goto, M., “Extraction of rice bran oil by supercritical carbon dioxide and solubility considerationâ€, Sep. Pur. Technol., vol. 125, pp. 319–325, 2014.

Kanda, H., Kamo, Y., Machmudah, S., Wahyudiono, Goto, M., “Extraction of Fucoxanthin from Raw Macroalgae excluding Drying and Cell Wall Disruption by Liquefied Dimethyl Etherâ€, Mar. Drugs, vol. 12, no. 5, pp. 2383-2396, 2014.

Goto, M., Kanda, H., Wahyudiono, Machmudah, S., “Extraction of carotenoids and lipids from algae by supercritical CO2 and subcritical dimethyl etherâ€, J. Supercrit. Fluids, vol. 96, pp. 245–251, 2015.

Won, KW., “Thermodynamic calculation of supercritical-fluid equilibria: new mixing rules for equation of stateâ€, Fluid Phase Equil., vol. 10, no. 2-3, pp.191-210, 1983.

Guclu-Ustundag, O., Temelli, F., “Solubility behavior of ternary systems of lipids, cosolvents and supercritical carbon dioxide and processing aspectsâ€, J. Supercrit. Fluids, vol. 36, no. 1, pp. 1–15, 2005.

Liu, J., Lin, S., Wang, Z., Wang, C., Wang, E., Zhang, Y., Liu, J., “Supercritical fluid extraction of flavonoids from Maydis stigma and its nitrite-scavenging abilityâ€, Food Bioprod. Process., vol. 89, no. 4, pp. 333–339, 2011.

Jinap, S., Kusnadi, AE., Akanda, MdJH., Rahman, RAbd., Ferdosh, S., Sarker, MdZI., “Effects of Moisture and pH on Supercritical Fluid Extraction of Cocoa Butterâ€, Food Bioprocess Technol., vol. 6, no. 9, pp. 2455–2465, 2013.

Durante, M., Lenucci, MS., Mita, G., “Supercritical Carbon Dioxide Extraction of Carotenoids from Pumpkin (Cucurbita spp.): A Reviewâ€, Int. J. Mol. Sci., vol. 15, no. 4, pp. 6725-6740, 2014.

Lee, YH., Charles, AL., Kung, HF., Ho, CT., Huang, TC., “Extraction of nobiletin and tangeretin from Citrus depressa Hayata by supercritical carbon dioxide with ethanol as modifierâ€, Ind. Crops Prod., vol. 31, no. 1, pp. 59–64, 2010.

Downloads

Published

2016-08-15

How to Cite

Kawamoto, Y., Suidou, Y., Suetsugu, T., Takamizu, A., Tanaka, M., Hoshino, M., Diono, W., Kanda, H., & Goto, M. (2016). Functional Ingredients Extraction from Citrus <i>Genkou </i>by Supercritical Carbon Dioxide. Asian Journal of Applied Sciences, 4(4). Retrieved from https://ajouronline.com/index.php/AJAS/article/view/3926

Issue

Section

Articles